Highly Efficient Trityl-Nitroxide Biradicals for Biomolecular High-Field Dynamic Nuclear Polarization. Cai, X., Lucini Paioni, A., Adler, A., Yao, R., Zhang, W., Beriashvili, D., Safeer, A., Gurinov, A., Rockenbauer, A., Song, Y., Baldus, M., & Liu, Y. Chemistry (Weinheim an Der Bergstrasse, Germany), 27(50):12758–12762, September, 2021. doi abstract bibtex Dynamic nuclear polarization (DNP) is a powerful method to enhance the sensitivity of solid-state magnetic nuclear resonance (ssNMR) spectroscopy. However, its biomolecular applications at high magnetic fields (preferably\textgreater14 T) have so far been limited by the intrinsically low efficiency of polarizing agents and sample preparation aspects. Herein, we report a new class of trityl-nitroxide biradicals, dubbed SNAPols that combine high DNP efficiency with greatly enhanced hydrophilicity. SNAPol-1, the best compound in the series, shows DNP enhancement factors at 18.8 T of more than 100 in small molecules and globular proteins and also exhibits strong DNP enhancements in membrane proteins and cellular preparations. By integrating optimal sensitivity and high resolution, we expect widespread applications of this new polarizing agent in high-field DNP/ssNMR spectroscopy, especially for complex biomolecules.
@article{cai_highly_2021,
title = {Highly {Efficient} {Trityl}-{Nitroxide} {Biradicals} for {Biomolecular} {High}-{Field} {Dynamic} {Nuclear} {Polarization}},
volume = {27},
issn = {1521-3765},
doi = {10.1002/chem.202102253},
abstract = {Dynamic nuclear polarization (DNP) is a powerful method to enhance the sensitivity of solid-state magnetic nuclear resonance (ssNMR) spectroscopy. However, its biomolecular applications at high magnetic fields (preferably{\textgreater}14 T) have so far been limited by the intrinsically low efficiency of polarizing agents and sample preparation aspects. Herein, we report a new class of trityl-nitroxide biradicals, dubbed SNAPols that combine high DNP efficiency with greatly enhanced hydrophilicity. SNAPol-1, the best compound in the series, shows DNP enhancement factors at 18.8 T of more than 100 in small molecules and globular proteins and also exhibits strong DNP enhancements in membrane proteins and cellular preparations. By integrating optimal sensitivity and high resolution, we expect widespread applications of this new polarizing agent in high-field DNP/ssNMR spectroscopy, especially for complex biomolecules.},
language = {eng},
number = {50},
journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)},
author = {Cai, Xinyi and Lucini Paioni, Alessandra and Adler, Agnes and Yao, Ru and Zhang, Wenxiao and Beriashvili, David and Safeer, Adil and Gurinov, Andrei and Rockenbauer, Antal and Song, Yuguang and Baldus, Marc and Liu, Yangping},
month = sep,
year = {2021},
pmid = {34181286},
keywords = {Magnetic Fields, Magnetic Resonance Spectroscopy, Membrane Proteins, Nitrogen Oxides, SNAPol, biradicals, dynamic nuclear polarization, electron paramagnetic resonance, polarizing agents, solid-state NMR spectroscopy},
pages = {12758--12762},
}
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Herein, we report a new class of trityl-nitroxide biradicals, dubbed SNAPols that combine high DNP efficiency with greatly enhanced hydrophilicity. SNAPol-1, the best compound in the series, shows DNP enhancement factors at 18.8 T of more than 100 in small molecules and globular proteins and also exhibits strong DNP enhancements in membrane proteins and cellular preparations. 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