Chitin degradation by Synechococcus WH7803

Chitin degradation by Synechococcus WH7803. Capovilla, G., Castro, K. G., Collani, S., Kearney, S. M., Kehoe, D. M., & Chisholm, S. W. Scientific Reports, 13(1):19944, November, 2023. Number: 1 Publisher: Nature Publishing Group

Paper doi abstract bibtex

Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.

@article{capovilla_chitin_2023,
	title = {Chitin degradation by {Synechococcus} {WH7803}},
	volume = {13},
	copyright = {2023 The Author(s)},
	issn = {2045-2322},
	url = {https://www.nature.com/articles/s41598-023-47332-0},
	doi = {10.1038/s41598-023-47332-0},
	abstract = {Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media of Synechococcus WH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.},
	language = {en},
	number = {1},
	urldate = {2023-11-17},
	journal = {Scientific Reports},
	author = {Capovilla, Giovanna and Castro, Kurt G. and Collani, Silvio and Kearney, Sean M. and Kehoe, David M. and Chisholm, Sallie W.},
	month = nov,
	year = {2023},
	note = {Number: 1
Publisher: Nature Publishing Group},
	keywords = {Ecology, Microbiology, Molecular biology},
	pages = {19944},
}

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