Advantages of Using Microbial Technology over Traditional Chemical Technology in Removal of Black Crusts from Stone Surfaces of Historical Monuments. Cappitelli, F., Toniolo, L., Sansonetti, A., Gulotta, D., Ranalli, G., Zanardini, E., & Sorlini, C. Applied and Environmental Microbiology, 73(17):5671–5675, September, 2007.
Advantages of Using Microbial Technology over Traditional Chemical Technology in Removal of Black Crusts from Stone Surfaces of Historical Monuments [link]Paper  doi  abstract   bibtex   
ABSTRACT This study compares two cleaning methods, one involving an ammonium carbonate-EDTA mixture and the other involving the sulfate-reducing bacterium Desulfovibrio vulgaris subsp. vulgaris ATCC 29579, for the removal of black crust (containing gypsum) on marble of the Milan Cathedral (Italy). In contrast to the chemical cleaning method, the biological procedure resulted in more homogeneous removal of the surface deposits and preserved the patina noble under the black crust. Whereas both of the treatments converted gypsum to calcite, allowing consolidation, the chemical treatment also formed undesirable sodium sulfate.
@article{cappitelli_advantages_2007,
	title = {Advantages of {Using} {Microbial} {Technology} over {Traditional} {Chemical} {Technology} in {Removal} of {Black} {Crusts} from {Stone} {Surfaces} of {Historical} {Monuments}},
	volume = {73},
	issn = {0099-2240, 1098-5336},
	url = {https://AEM.asm.org/content/73/17/5671},
	doi = {10.1128/AEM.00394-07},
	abstract = {ABSTRACT
            
              This study compares two cleaning methods, one involving an ammonium carbonate-EDTA mixture and the other involving the sulfate-reducing bacterium
              Desulfovibrio vulgaris
              subsp.
              vulgaris
              ATCC 29579, for the removal of black crust (containing gypsum) on marble of the Milan Cathedral (Italy). In contrast to the chemical cleaning method, the biological procedure resulted in more homogeneous removal of the surface deposits and preserved the patina noble under the black crust. Whereas both of the treatments converted gypsum to calcite, allowing consolidation, the chemical treatment also formed undesirable sodium sulfate.},
	language = {en},
	number = {17},
	urldate = {2021-05-07},
	journal = {Applied and Environmental Microbiology},
	author = {Cappitelli, Francesca and Toniolo, Lucia and Sansonetti, Antonio and Gulotta, Davide and Ranalli, Giancarlo and Zanardini, Elisabetta and Sorlini, Claudia},
	month = sep,
	year = {2007},
	pages = {5671--5675},
}

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