Study of the reactive dendritic cells in small B-cell lymphoproliferations of the skin. Carbonnelle, A., Martin-Garcia, N., Ortonne, N., Laroche, L., Bagot, M., Molinier-Frenkel, V., & Wechsler, J. Virchows Arch, 450(4):441-447, 2007.
Study of the reactive dendritic cells in small B-cell lymphoproliferations of the skin [link]Website  abstract   bibtex   
Distinguishing between low-grade primary cutaneous B-cell lymphoma (LG-pCBCL) and cutaneous lymphoid hyperplasia (CLH) based on histological features is often difficult. CLH lesions contain numerous reactive cells of the histiocyte lineage [Langerhans cells (LC), dermal dendritic cells (DDC), and macrophages], which are also often present in CBCL. The aim of this study was to determine whether immunohistochemical detection of those cells could help differentiate between CLH and LG-pCBCL. We determined the percentages of those histiocytic cells in the dermal infiltrates of 45 cases of cutaneous lymphoproliferations comprising 16 CLH and 29 LG-pCBCL (19 follicle-center cell lymphomas and 10 marginal zone lymphomas) by immunohistochemical labeling with antibodies to CD1a, FXIIIa, and CD68 to respectively detect LC, DDC, and macrophages. To avoid observer-dependent bias, an automated morphometric analysis method was used to recognize immunoreactive cells and calculate their percentages within the infiltrate. FXIIIa(+) cells were significatively more frequent in CLH than in LG-pCBCL, whereas CD1a(+) and CD68(+) cell frequencies were comparable in the two groups. The results of our study suggest that DDC might play an important role in the genesis of cutaneous lymphomas.
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 title = {Study of the reactive dendritic cells in small B-cell lymphoproliferations of the skin},
 type = {article},
 year = {2007},
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 keywords = {Adolescent,Adult,Aged,Aged, 80 and over,Antigens, CD/analysis,Antigens, CD1/analysis,Antigens, Differentiation, Myelomonocytic/analysis,Dendritic Cells/chemistry/*pathology,Diagnosis, Differential,Factor XIIIa/analysis,Female,Humans,Immunohistochemistry,Lymphoma, B-Cell/metabolism/*pathology,Male,Middle Aged,Pseudolymphoma/metabolism/pathology,Skin Diseases/metabolism/pathology,Skin Neoplasms/metabolism/*pathology},
 pages = {441-447},
 volume = {450},
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 edition = {2007/03/23},
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 language = {eng},
 notes = {<m:note>Carbonnelle, Amelie<m:linebreak/>Martin-Garcia, Nadine<m:linebreak/>Ortonne, Nicolas<m:linebreak/>Laroche, Liliane<m:linebreak/>Bagot, Martine<m:linebreak/>Molinier-Frenkel, Valerie<m:linebreak/>Wechsler, Janine<m:linebreak/>Germany<m:linebreak/>Virchows Archiv : an international journal of pathology<m:linebreak/>Virchows Arch. 2007 Apr;450(4):441-7. Epub 2007 Feb 15.</m:note>},
 abstract = {Distinguishing between low-grade primary cutaneous B-cell lymphoma (LG-pCBCL) and cutaneous lymphoid hyperplasia (CLH) based on histological features is often difficult. CLH lesions contain numerous reactive cells of the histiocyte lineage [Langerhans cells (LC), dermal dendritic cells (DDC), and macrophages], which are also often present in CBCL. The aim of this study was to determine whether immunohistochemical detection of those cells could help differentiate between CLH and LG-pCBCL. We determined the percentages of those histiocytic cells in the dermal infiltrates of 45 cases of cutaneous lymphoproliferations comprising 16 CLH and 29 LG-pCBCL (19 follicle-center cell lymphomas and 10 marginal zone lymphomas) by immunohistochemical labeling with antibodies to CD1a, FXIIIa, and CD68 to respectively detect LC, DDC, and macrophages. To avoid observer-dependent bias, an automated morphometric analysis method was used to recognize immunoreactive cells and calculate their percentages within the infiltrate. FXIIIa(+) cells were significatively more frequent in CLH than in LG-pCBCL, whereas CD1a(+) and CD68(+) cell frequencies were comparable in the two groups. The results of our study suggest that DDC might play an important role in the genesis of cutaneous lymphomas.},
 bibtype = {article},
 author = {Carbonnelle, A and Martin-Garcia, N and Ortonne, N and Laroche, L and Bagot, M and Molinier-Frenkel, V and Wechsler, J},
 journal = {Virchows Arch},
 number = {4}
}

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