{"_id":"gehW7F4jSQ4x76KbH","bibbaseid":"carolinediener-daliaalansary-barbarawalchrckheim-friedrichgrsser-stefanierheinheimer-hanspeterlenhof-modulationofintracellularcalciumsignalingbymicrorna34a5p-2018","authorIDs":[],"author_short":["Caroline Diener, M. H.","Dalia Alansary, V. P.","Barbara Walch-Rückheim, J. M.","Friedrich Grässer, T. F.","Stefanie Rheinheimer, B. A N.","Hans-Peter Lenhof, A. K."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Caroline","Diener"],"firstnames":["Martin","Hart"],"suffixes":[]},{"propositions":[],"lastnames":["Dalia","Alansary"],"firstnames":["Vanessa","Poth"],"suffixes":[]},{"propositions":[],"lastnames":["Barbara","Walch-Rückheim"],"firstnames":["Jennifer","Menegatti"],"suffixes":[]},{"propositions":[],"lastnames":["Friedrich","Grässer"],"firstnames":["Tobias","Fehlmann"],"suffixes":[]},{"propositions":[],"lastnames":["Stefanie","Rheinheimer"],"firstnames":["Barbara","A","Niemeyer"],"suffixes":[]},{"propositions":[],"lastnames":["Hans-Peter","Lenhof"],"firstnames":["Andreas","Keller"],"suffixes":[]}],"title":"Modulation of intracellular calcium signaling by microRNA-34a-5p","journal":"Cell death & disease","year":"2018","volume":"9","pages":"1008","month":"September","issn":"1008","abstract":"Adjusting intracellular calcium signaling is an important feature in the regulation of immune cell function and survival. Here we show that miR-34a-5p, a small non-coding RNA that is deregulated in many common diseases, is a regulator of store-operated Ca2+ entry (SOCE) and calcineurin signaling. Upon miR-34a-5p overexpression, we observed both a decreased depletion of ER calcium content and a decreased Ca2+ influx through Ca2+ release-activated Ca2+ channels. Based on an in silico target prediction we identified multiple miR-34a-5p target genes within both pathways that are implicated in the balance between T-cell activation and apoptosis including ITPR2, CAMLG, STIM1, ORAI3, RCAN1, PPP3R1, and NFATC4. Functional analysis revealed a decrease in Ca2+ activated calcineurin pathway activity measured by a reduced IL-2 secretion due to miR-34a-5p overexpression. Impacting SOCE and/or downstream calcineurin/NFAT signaling by miR-34a-5p offers a possible future approach to manipulate immune cells for clinical interventions.","doi":"10.1038/s41419-018-1050-7","issn-linking":"1008","issue":"10","pii":"10.1038/s41419-018-1050-7","publisher":"Nature Publishing Group","bibtex":"@Article{Diener2018,\n author = {Caroline Diener, Martin Hart, Dalia Alansary, Vanessa Poth, Barbara Walch-Rückheim, Jennifer Menegatti, Friedrich Grässer, Tobias Fehlmann, Stefanie Rheinheimer, Barbara A Niemeyer, Hans-Peter Lenhof, Andreas Keller, Eckart Meese},\n title = {Modulation of intracellular calcium signaling by microRNA-34a-5p},\n journal = {Cell death & disease},\n year = {2018},\n volume = {9},\n pages = {1008},\n month = sep,\n issn = {1008},\n abstract = {Adjusting intracellular calcium signaling is an important feature in the regulation of immune cell function and survival. Here we show that miR-34a-5p, a small non-coding RNA that is deregulated in many common diseases, is a regulator of store-operated Ca2+ entry (SOCE) and calcineurin signaling. Upon miR-34a-5p overexpression, we observed both a decreased depletion of ER calcium content and a decreased Ca2+ influx through Ca2+ release-activated Ca2+ channels. Based on an in silico target prediction we identified multiple miR-34a-5p target genes within both pathways that are implicated in the balance between T-cell activation and apoptosis including ITPR2, CAMLG, STIM1, ORAI3, RCAN1, PPP3R1, and NFATC4. Functional analysis revealed a decrease in Ca2+ activated calcineurin pathway activity measured by a reduced IL-2 secretion due to miR-34a-5p overexpression. Impacting SOCE and/or downstream calcineurin/NFAT signaling by miR-34a-5p offers a possible future approach to manipulate immune cells for clinical interventions.},\n doi = {10.1038/s41419-018-1050-7},\n issn-linking = {1008},\n issue = {10},\n pii = {10.1038/s41419-018-1050-7},\n publisher = {Nature Publishing Group},\n}\n\n","author_short":["Caroline Diener, M. H.","Dalia Alansary, V. P.","Barbara Walch-Rückheim, J. M.","Friedrich Grässer, T. F.","Stefanie Rheinheimer, B. A N.","Hans-Peter Lenhof, A. K."],"key":"Diener2018","id":"Diener2018","bibbaseid":"carolinediener-daliaalansary-barbarawalchrckheim-friedrichgrsser-stefanierheinheimer-hanspeterlenhof-modulationofintracellularcalciumsignalingbymicrorna34a5p-2018","role":"author","urls":{},"downloads":0,"html":""},"bibtype":"article","biburl":"https://www.ccb.uni-saarland.de/wp-content/uploads/2020/01/references.bib_.txt","creationDate":"2020-01-08T09:44:14.469Z","downloads":0,"keywords":[],"search_terms":["modulation","intracellular","calcium","signaling","microrna","34a","caroline diener","dalia alansary","barbara walch-rückheim","friedrich grässer","stefanie rheinheimer","hans-peter lenhof"],"title":"Modulation of intracellular calcium signaling by microRNA-34a-5p","year":2018,"dataSources":["ojGAdShp9TLBmYGi7"]}