Potential of Magnetic Hyperthermia to Stimulate Localized Immune Activation. Carter, T. J., Agliardi, G., Lin, F., Ellis, M., Jones, C., Robson, M., Richard-Londt, A., Southern, P., Lythgoe, M., Zaw Thin, M., Ryzhov, V., de Rosales, R. T. M., Gruettner, C., Abdollah, M. R. A., Pedley, R. B., Pankhurst, Q. A., Kalber, T. L., Brandner, S., Quezada, S., Mulholland, P., Shevtsov, M., & Chester, K. Small (Weinheim an der Bergstrasse, Germany), 17:e2005241, April, 2021. doi abstract bibtex Magnetic hyperthermia (MH) harnesses the heat-releasing properties of superparamagnetic iron oxide nanoparticles (SPIONs) and has potential to stimulate immune activation in the tumor microenvironment whilst sparing surrounding normal tissues. To assess feasibility of localized MH in vivo, SPIONs are injected intratumorally and their fate tracked by Zirconium-89-positron emission tomography, histological analysis, and electron microscopy. Experiments show that an average of 49% (21-87%, n = 9) of SPIONs are retained within the tumor or immediately surrounding tissue. In situ heating is subsequently generated by exposure to an externally applied alternating magnetic field and monitored by thermal imaging. Tissue response to hyperthermia, measured by immunohistochemical image analysis, reveals specific and localized heat-shock protein expression following treatment. Tumor growth inhibition is also observed. To evaluate the potential effects of MH on the immune landscape, flow cytometry is used to characterize immune cells from excised tumors and draining lymph nodes. Results show an influx of activated cytotoxic T cells, alongside an increase in proliferating regulatory T cells, following treatment. Complementary changes are found in draining lymph nodes. In conclusion, results indicate that biologically reactive MH is achievable in vivo and can generate localized changes consistent with an anti-tumor immune response.
@Article{Carter2021,
author = {Carter, Thomas J. and Agliardi, Giulia and Lin, Fang-Yu and Ellis, Matthew and Jones, Clare and Robson, Mathew and Richard-Londt, Angela and Southern, Paul and Lythgoe, Mark and Zaw Thin, May and Ryzhov, Vyacheslav and de Rosales, Rafael T. M. and Gruettner, Cordula and Abdollah, Maha R. A. and Pedley, R. Barbara and Pankhurst, Quentin A. and Kalber, Tammy L. and Brandner, Sebastian and Quezada, Sergio and Mulholland, Paul and Shevtsov, Maxim and Chester, Kerry},
journal = {Small (Weinheim an der Bergstrasse, Germany)},
title = {Potential of Magnetic Hyperthermia to Stimulate Localized Immune Activation.},
year = {2021},
issn = {1613-6829},
month = apr,
pages = {e2005241},
volume = {17},
abstract = {Magnetic hyperthermia (MH) harnesses the heat-releasing properties of superparamagnetic iron oxide nanoparticles (SPIONs) and has potential to stimulate immune activation in the tumor microenvironment whilst sparing surrounding normal tissues. To assess feasibility of localized MH in vivo, SPIONs are injected intratumorally and their fate tracked by Zirconium-89-positron emission tomography, histological analysis, and electron microscopy. Experiments show that an average of 49% (21-87%, n = 9) of SPIONs are retained within the tumor or immediately surrounding tissue. In situ heating is subsequently generated by exposure to an externally applied alternating magnetic field and monitored by thermal imaging. Tissue response to hyperthermia, measured by immunohistochemical image analysis, reveals specific and localized heat-shock protein expression following treatment. Tumor growth inhibition is also observed. To evaluate the potential effects of MH on the immune landscape, flow cytometry is used to characterize immune cells from excised tumors and draining lymph nodes. Results show an influx of activated cytotoxic T cells, alongside an increase in proliferating regulatory T cells, following treatment. Complementary changes are found in draining lymph nodes. In conclusion, results indicate that biologically reactive MH is achievable in vivo and can generate localized changes consistent with an anti-tumor immune response.},
chemicals = {Ferric Compounds, Magnetite Nanoparticles},
citation-subset = {IM},
completed = {2021-07-14},
country = {Germany},
doi = {10.1002/smll.202005241},
issn-linking = {1613-6810},
issue = {14},
keywords = {Ferric Compounds; Humans; Hyperthermia; Hyperthermia, Induced; Magnetic Fields; Magnetics; Magnetite Nanoparticles; biological response; heat-shock protein 70; immune stimulation; magnetic hyperthermia; superparamagnetic iron oxide nanoparticles},
nlm-id = {101235338},
owner = {NLM},
pmid = {33734595},
pubmodel = {Print-Electronic},
pubstate = {ppublish},
revised = {2021-07-14},
}
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L.","Brandner, S.","Quezada, S.","Mulholland, P.","Shevtsov, M.","Chester, K."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Carter"],"firstnames":["Thomas","J."],"suffixes":[]},{"propositions":[],"lastnames":["Agliardi"],"firstnames":["Giulia"],"suffixes":[]},{"propositions":[],"lastnames":["Lin"],"firstnames":["Fang-Yu"],"suffixes":[]},{"propositions":[],"lastnames":["Ellis"],"firstnames":["Matthew"],"suffixes":[]},{"propositions":[],"lastnames":["Jones"],"firstnames":["Clare"],"suffixes":[]},{"propositions":[],"lastnames":["Robson"],"firstnames":["Mathew"],"suffixes":[]},{"propositions":[],"lastnames":["Richard-Londt"],"firstnames":["Angela"],"suffixes":[]},{"propositions":[],"lastnames":["Southern"],"firstnames":["Paul"],"suffixes":[]},{"propositions":[],"lastnames":["Lythgoe"],"firstnames":["Mark"],"suffixes":[]},{"propositions":[],"lastnames":["Zaw","Thin"],"firstnames":["May"],"suffixes":[]},{"propositions":[],"lastnames":["Ryzhov"],"firstnames":["Vyacheslav"],"suffixes":[]},{"propositions":["de"],"lastnames":["Rosales"],"firstnames":["Rafael","T.","M."],"suffixes":[]},{"propositions":[],"lastnames":["Gruettner"],"firstnames":["Cordula"],"suffixes":[]},{"propositions":[],"lastnames":["Abdollah"],"firstnames":["Maha","R.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Pedley"],"firstnames":["R.","Barbara"],"suffixes":[]},{"propositions":[],"lastnames":["Pankhurst"],"firstnames":["Quentin","A."],"suffixes":[]},{"propositions":[],"lastnames":["Kalber"],"firstnames":["Tammy","L."],"suffixes":[]},{"propositions":[],"lastnames":["Brandner"],"firstnames":["Sebastian"],"suffixes":[]},{"propositions":[],"lastnames":["Quezada"],"firstnames":["Sergio"],"suffixes":[]},{"propositions":[],"lastnames":["Mulholland"],"firstnames":["Paul"],"suffixes":[]},{"propositions":[],"lastnames":["Shevtsov"],"firstnames":["Maxim"],"suffixes":[]},{"propositions":[],"lastnames":["Chester"],"firstnames":["Kerry"],"suffixes":[]}],"journal":"Small (Weinheim an der Bergstrasse, Germany)","title":"Potential of Magnetic Hyperthermia to Stimulate Localized Immune Activation.","year":"2021","issn":"1613-6829","month":"April","pages":"e2005241","volume":"17","abstract":"Magnetic hyperthermia (MH) harnesses the heat-releasing properties of superparamagnetic iron oxide nanoparticles (SPIONs) and has potential to stimulate immune activation in the tumor microenvironment whilst sparing surrounding normal tissues. To assess feasibility of localized MH in vivo, SPIONs are injected intratumorally and their fate tracked by Zirconium-89-positron emission tomography, histological analysis, and electron microscopy. Experiments show that an average of 49% (21-87%, n = 9) of SPIONs are retained within the tumor or immediately surrounding tissue. In situ heating is subsequently generated by exposure to an externally applied alternating magnetic field and monitored by thermal imaging. Tissue response to hyperthermia, measured by immunohistochemical image analysis, reveals specific and localized heat-shock protein expression following treatment. Tumor growth inhibition is also observed. To evaluate the potential effects of MH on the immune landscape, flow cytometry is used to characterize immune cells from excised tumors and draining lymph nodes. Results show an influx of activated cytotoxic T cells, alongside an increase in proliferating regulatory T cells, following treatment. 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M. and Gruettner, Cordula and Abdollah, Maha R. A. and Pedley, R. Barbara and Pankhurst, Quentin A. and Kalber, Tammy L. and Brandner, Sebastian and Quezada, Sergio and Mulholland, Paul and Shevtsov, Maxim and Chester, Kerry},\n journal = {Small (Weinheim an der Bergstrasse, Germany)},\n title = {Potential of Magnetic Hyperthermia to Stimulate Localized Immune Activation.},\n year = {2021},\n issn = {1613-6829},\n month = apr,\n pages = {e2005241},\n volume = {17},\n abstract = {Magnetic hyperthermia (MH) harnesses the heat-releasing properties of superparamagnetic iron oxide nanoparticles (SPIONs) and has potential to stimulate immune activation in the tumor microenvironment whilst sparing surrounding normal tissues. To assess feasibility of localized MH in vivo, SPIONs are injected intratumorally and their fate tracked by Zirconium-89-positron emission tomography, histological analysis, and electron microscopy. 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