Morphomechanical alterations induced by transforming growth factor-β1 in epithelial breast cancer cells. Cascione, M., De Matteis, V., Toma, C., & Leporatti, S. Cancers, MDPI AG, 2018. cited By 5
Paper doi abstract bibtex The Epithelial to mesenchymal transition (EMT) is the process that drives epithelial tumor cells to acquire an invasive phenotype. The role of transforming growth factor-β1 (TGF-β1) in EMT is still debated. We used confocal laser scanning microscopy and scanning force spectroscopy to perform a morphomechanical analysis on epithelial breast cancer cells (MCF-7), comparing them before and after TGF-β1 exogenous stimulation (5 ng/mL for 48 h). After TGF-β1 treatment, loss of cell–cell adherence (mainly due to the reduction of E-cadherin expression of about 24%) and disaggregation of actin cortical fibers were observed in treated MCF-7. In addition, TGF-β1 induced an alteration of MCF-7 nuclei morphology as well as a decrease in the Young’s modulus, owing to a rearrangement that involved the cytoskeletal networks and the nuclear region. These relevant variations in morphological features and mechanical properties, elicited by TGF-β1, suggested an increased capacity of MCF-7 to migrate, which was confirmed by a wound healing assay. By means of our biophysical approach, we highlighted the malignant progression of breast cancer cells induced by TGF-β1 exposure. We are confirming TGF-β1’s role in EMT by means of morphomechanical evidence that could represent a turning point in understanding the molecular mechanisms involved in cancer progression. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
@ARTICLE{Cascione2018,
author={Cascione, M. and De Matteis, V. and Toma, C.C. and Leporatti, S.},
title={Morphomechanical alterations induced by transforming growth factor-β1 in epithelial breast cancer cells},
journal={Cancers},
year={2018},
volume={10},
number={7},
doi={10.3390/cancers10070234},
art_number={234},
note={cited By 5},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85050698293&doi=10.3390%2fcancers10070234&partnerID=40&md5=fa74d9d34c0e95aff9bf0e1e73227a51},
abstract={The Epithelial to mesenchymal transition (EMT) is the process that drives epithelial tumor cells to acquire an invasive phenotype. The role of transforming growth factor-β1 (TGF-β1) in EMT is still debated. We used confocal laser scanning microscopy and scanning force spectroscopy to perform a morphomechanical analysis on epithelial breast cancer cells (MCF-7), comparing them before and after TGF-β1 exogenous stimulation (5 ng/mL for 48 h). After TGF-β1 treatment, loss of cell–cell adherence (mainly due to the reduction of E-cadherin expression of about 24%) and disaggregation of actin cortical fibers were observed in treated MCF-7. In addition, TGF-β1 induced an alteration of MCF-7 nuclei morphology as well as a decrease in the Young’s modulus, owing to a rearrangement that involved the cytoskeletal networks and the nuclear region. These relevant variations in morphological features and mechanical properties, elicited by TGF-β1, suggested an increased capacity of MCF-7 to migrate, which was confirmed by a wound healing assay. By means of our biophysical approach, we highlighted the malignant progression of breast cancer cells induced by TGF-β1 exposure. We are confirming TGF-β1’s role in EMT by means of morphomechanical evidence that could represent a turning point in understanding the molecular mechanisms involved in cancer progression. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.},
publisher={MDPI AG},
issn={20726694},
document_type={Article},
source={Scopus},
}
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