Morphomechanical Alterations Induced by Transforming Growth Factor-beta 1 in Epithelial Breast Cancer Cells. Cascione, M., De Matteis, V., Toma, C. C., & Leporatti, S. CANCERS, JUL, 2018.
doi  abstract   bibtex   
The Epithelial to mesenchymal transition (EMT) is the process that drives epithelial tumor cells to acquire an invasive phenotype. The role of transforming growth factor-beta 1 (TGF-beta 1) in EMT is still debated. We used confocal laser scanning microscopy and scanning force spectroscopy to perform a morphomechanical analysis on epithelial breast cancer cells (MCF-7), comparing them before and after TGF-beta 1 exogenous stimulation (5 ng/mL for 48 h). After TGF-beta 1 treatment, loss of cell-cell adherence (mainly due to the reduction of E-cadherin expression of about 24%) and disaggregation of actin cortical fibers were observed in treated MCF-7. In addition, TGF-beta 1 induced an alteration of MCF-7 nuclei morphology as well as a decrease in the Young's modulus, owing to a rearrangement that involved the cytoskeletal networks and the nuclear region. These relevant variations in morphological features and mechanical properties, elicited by TGF-beta 1, suggested an increased capacity of MCF-7 to migrate, which was confirmed by a wound healing assay. By means of our biophysical approach, we highlighted the malignant progression of breast cancer cells induced by TGF-beta 1 exposure. We are confirming TGF-beta 1's role in EMT by means of morphomechanical evidence that could represent a turning point in understanding the molecular mechanisms involved in cancer progression.
@article{ ISI:000445632200025,
Author = {Cascione, Mariafrancesca and De Matteis, Valeria and Toma, Chiara C. and
   Leporatti, Stefano},
Title = {{Morphomechanical Alterations Induced by Transforming Growth Factor-beta
   1 in Epithelial Breast Cancer Cells}},
Journal = {{CANCERS}},
Year = {{2018}},
Volume = {{10}},
Number = {{7}},
Month = {{JUL}},
Abstract = {{The Epithelial to mesenchymal transition (EMT) is the process that
   drives epithelial tumor cells to acquire an invasive phenotype. The role
   of transforming growth factor-beta 1 (TGF-beta 1) in EMT is still
   debated. We used confocal laser scanning microscopy and scanning force
   spectroscopy to perform a morphomechanical analysis on epithelial breast
   cancer cells (MCF-7), comparing them before and after TGF-beta 1
   exogenous stimulation (5 ng/mL for 48 h). After TGF-beta 1 treatment,
   loss of cell-cell adherence (mainly due to the reduction of E-cadherin
   expression of about 24\%) and disaggregation of actin cortical fibers
   were observed in treated MCF-7. In addition, TGF-beta 1 induced an
   alteration of MCF-7 nuclei morphology as well as a decrease in the
   Young's modulus, owing to a rearrangement that involved the cytoskeletal
   networks and the nuclear region. These relevant variations in
   morphological features and mechanical properties, elicited by TGF-beta
   1, suggested an increased capacity of MCF-7 to migrate, which was
   confirmed by a wound healing assay. By means of our biophysical
   approach, we highlighted the malignant progression of breast cancer
   cells induced by TGF-beta 1 exposure. We are confirming TGF-beta 1's
   role in EMT by means of morphomechanical evidence that could represent a
   turning point in understanding the molecular mechanisms involved in
   cancer progression.}},
DOI = {{10.3390/cancers10070234}},
Article-Number = {{234}},
ISSN = {{2072-6694}},
ResearcherID-Numbers = {{Leporatti, Stefano/E-1597-2011
   De Matteis, Valeria/J-4530-2019}},
ORCID-Numbers = {{Leporatti, Stefano/0000-0001-5912-7565
   De Matteis, Valeria/0000-0003-2204-8817}},
Unique-ID = {{ISI:000445632200025}},
}

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