Morphomechanical Alterations Induced by Transforming Growth Factor-beta 1 in Epithelial Breast Cancer Cells. Cascione, M., De Matteis, V., Toma, C. C., & Leporatti, S. CANCERS, JUL, 2018. doi abstract bibtex The Epithelial to mesenchymal transition (EMT) is the process that drives epithelial tumor cells to acquire an invasive phenotype. The role of transforming growth factor-beta 1 (TGF-beta 1) in EMT is still debated. We used confocal laser scanning microscopy and scanning force spectroscopy to perform a morphomechanical analysis on epithelial breast cancer cells (MCF-7), comparing them before and after TGF-beta 1 exogenous stimulation (5 ng/mL for 48 h). After TGF-beta 1 treatment, loss of cell-cell adherence (mainly due to the reduction of E-cadherin expression of about 24%) and disaggregation of actin cortical fibers were observed in treated MCF-7. In addition, TGF-beta 1 induced an alteration of MCF-7 nuclei morphology as well as a decrease in the Young's modulus, owing to a rearrangement that involved the cytoskeletal networks and the nuclear region. These relevant variations in morphological features and mechanical properties, elicited by TGF-beta 1, suggested an increased capacity of MCF-7 to migrate, which was confirmed by a wound healing assay. By means of our biophysical approach, we highlighted the malignant progression of breast cancer cells induced by TGF-beta 1 exposure. We are confirming TGF-beta 1's role in EMT by means of morphomechanical evidence that could represent a turning point in understanding the molecular mechanisms involved in cancer progression.
@article{ ISI:000445632200025,
Author = {Cascione, Mariafrancesca and De Matteis, Valeria and Toma, Chiara C. and
Leporatti, Stefano},
Title = {{Morphomechanical Alterations Induced by Transforming Growth Factor-beta
1 in Epithelial Breast Cancer Cells}},
Journal = {{CANCERS}},
Year = {{2018}},
Volume = {{10}},
Number = {{7}},
Month = {{JUL}},
Abstract = {{The Epithelial to mesenchymal transition (EMT) is the process that
drives epithelial tumor cells to acquire an invasive phenotype. The role
of transforming growth factor-beta 1 (TGF-beta 1) in EMT is still
debated. We used confocal laser scanning microscopy and scanning force
spectroscopy to perform a morphomechanical analysis on epithelial breast
cancer cells (MCF-7), comparing them before and after TGF-beta 1
exogenous stimulation (5 ng/mL for 48 h). After TGF-beta 1 treatment,
loss of cell-cell adherence (mainly due to the reduction of E-cadherin
expression of about 24\%) and disaggregation of actin cortical fibers
were observed in treated MCF-7. In addition, TGF-beta 1 induced an
alteration of MCF-7 nuclei morphology as well as a decrease in the
Young's modulus, owing to a rearrangement that involved the cytoskeletal
networks and the nuclear region. These relevant variations in
morphological features and mechanical properties, elicited by TGF-beta
1, suggested an increased capacity of MCF-7 to migrate, which was
confirmed by a wound healing assay. By means of our biophysical
approach, we highlighted the malignant progression of breast cancer
cells induced by TGF-beta 1 exposure. We are confirming TGF-beta 1's
role in EMT by means of morphomechanical evidence that could represent a
turning point in understanding the molecular mechanisms involved in
cancer progression.}},
DOI = {{10.3390/cancers10070234}},
Article-Number = {{234}},
ISSN = {{2072-6694}},
ResearcherID-Numbers = {{Leporatti, Stefano/E-1597-2011
De Matteis, Valeria/J-4530-2019}},
ORCID-Numbers = {{Leporatti, Stefano/0000-0001-5912-7565
De Matteis, Valeria/0000-0003-2204-8817}},
Unique-ID = {{ISI:000445632200025}},
}
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C.","Leporatti, S."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Cascione"],"firstnames":["Mariafrancesca"],"suffixes":[]},{"propositions":[],"lastnames":["De","Matteis"],"firstnames":["Valeria"],"suffixes":[]},{"propositions":[],"lastnames":["Toma"],"firstnames":["Chiara","C."],"suffixes":[]},{"propositions":[],"lastnames":["Leporatti"],"firstnames":["Stefano"],"suffixes":[]}],"title":"Morphomechanical Alterations Induced by Transforming Growth Factor-beta 1 in Epithelial Breast Cancer Cells","journal":"CANCERS","year":"2018","volume":"10","number":"7","month":"JUL","abstract":"The Epithelial to mesenchymal transition (EMT) is the process that drives epithelial tumor cells to acquire an invasive phenotype. The role of transforming growth factor-beta 1 (TGF-beta 1) in EMT is still debated. We used confocal laser scanning microscopy and scanning force spectroscopy to perform a morphomechanical analysis on epithelial breast cancer cells (MCF-7), comparing them before and after TGF-beta 1 exogenous stimulation (5 ng/mL for 48 h). After TGF-beta 1 treatment, loss of cell-cell adherence (mainly due to the reduction of E-cadherin expression of about 24%) and disaggregation of actin cortical fibers were observed in treated MCF-7. In addition, TGF-beta 1 induced an alteration of MCF-7 nuclei morphology as well as a decrease in the Young's modulus, owing to a rearrangement that involved the cytoskeletal networks and the nuclear region. These relevant variations in morphological features and mechanical properties, elicited by TGF-beta 1, suggested an increased capacity of MCF-7 to migrate, which was confirmed by a wound healing assay. By means of our biophysical approach, we highlighted the malignant progression of breast cancer cells induced by TGF-beta 1 exposure. We are confirming TGF-beta 1's role in EMT by means of morphomechanical evidence that could represent a turning point in understanding the molecular mechanisms involved in cancer progression.","doi":"10.3390/cancers10070234","article-number":"234","issn":"2072-6694","researcherid-numbers":"Leporatti, Stefano/E-1597-2011 De Matteis, Valeria/J-4530-2019","orcid-numbers":"Leporatti, Stefano/0000-0001-5912-7565 De Matteis, Valeria/0000-0003-2204-8817","unique-id":"ISI:000445632200025","bibtex":"@article{ ISI:000445632200025,\nAuthor = {Cascione, Mariafrancesca and De Matteis, Valeria and Toma, Chiara C. and\n Leporatti, Stefano},\nTitle = {{Morphomechanical Alterations Induced by Transforming Growth Factor-beta\n 1 in Epithelial Breast Cancer Cells}},\nJournal = {{CANCERS}},\nYear = {{2018}},\nVolume = {{10}},\nNumber = {{7}},\nMonth = {{JUL}},\nAbstract = {{The Epithelial to mesenchymal transition (EMT) is the process that\n drives epithelial tumor cells to acquire an invasive phenotype. The role\n of transforming growth factor-beta 1 (TGF-beta 1) in EMT is still\n debated. We used confocal laser scanning microscopy and scanning force\n spectroscopy to perform a morphomechanical analysis on epithelial breast\n cancer cells (MCF-7), comparing them before and after TGF-beta 1\n exogenous stimulation (5 ng/mL for 48 h). After TGF-beta 1 treatment,\n loss of cell-cell adherence (mainly due to the reduction of E-cadherin\n expression of about 24\\%) and disaggregation of actin cortical fibers\n were observed in treated MCF-7. In addition, TGF-beta 1 induced an\n alteration of MCF-7 nuclei morphology as well as a decrease in the\n Young's modulus, owing to a rearrangement that involved the cytoskeletal\n networks and the nuclear region. These relevant variations in\n morphological features and mechanical properties, elicited by TGF-beta\n 1, suggested an increased capacity of MCF-7 to migrate, which was\n confirmed by a wound healing assay. 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