Blind patch clamp recordings in embryonic and adult mammalian brain slices. Castañeda-Castellanos, D. R, Flint, A. C, & Kriegstein, A. R Nat Protoc, 1(2):532–542, England, 2006.
abstract   bibtex   
To obtain electrophysiological recordings in brain slices, sophisticated and expensive pieces of equipment can be used. However, costly microscope equipment with infrared differential interference contrast optics is not always necessary or even desirable. For instance, obtaining a randomized unbiased sample in a given preparation would be better accomplished if cells were not directly visualized before recording. In addition, some preparations require thick slices, and direct visualization is not possible. Here we describe a protocol for the 'blind patch clamp method' that we developed several years ago to perform electrophysiological recordings in mammalian brain slices using a standard patch clamp amplifier, dissecting microscope and recording chamber. Overall, it takes approximately 3-4 h to set up this procedure.
@ARTICLE{Castaneda-Castellanos2006-we,
  title    = "Blind patch clamp recordings in embryonic and adult mammalian
              brain slices",
  author   = "Casta{\~n}eda-Castellanos, David R and Flint, Alexander C and
              Kriegstein, Arnold R",
  abstract = "To obtain electrophysiological recordings in brain slices,
              sophisticated and expensive pieces of equipment can be used.
              However, costly microscope equipment with infrared differential
              interference contrast optics is not always necessary or even
              desirable. For instance, obtaining a randomized unbiased sample
              in a given preparation would be better accomplished if cells were
              not directly visualized before recording. In addition, some
              preparations require thick slices, and direct visualization is
              not possible. Here we describe a protocol for the 'blind patch
              clamp method' that we developed several years ago to perform
              electrophysiological recordings in mammalian brain slices using a
              standard patch clamp amplifier, dissecting microscope and
              recording chamber. Overall, it takes approximately 3-4 h to set
              up this procedure.",
  journal  = "Nat Protoc",
  volume   =  1,
  number   =  2,
  pages    = "532--542",
  year     =  2006,
  address  = "England",
  language = "en"
}

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