@article{
 title = {Metal concentrations, sperm motility, and RNA/DNA ratio in two echinoderm species from a highly contaminated fjord (the Sørfjord, Norway).},
 type = {article},
 year = {2008},
 identifiers = {[object Object]},
 keywords = {Animals,Asterias,Asterias: chemistry,Asterias: drug effects,DNA,DNA: analysis,Environmental Monitoring,Geologic Sediments,Geologic Sediments: chemistry,Heavy,Heavy: analysis,Heavy: toxicity,Male,Metals,Norway,RNA,RNA: analysis,Reproducibility of Results,Sea Urchins,Sea Urchins: chemistry,Sea Urchins: drug effects,Species Specificity,Sperm Count,Sperm Motility,Sperm Motility: drug effects,Spermatozoa,Spermatozoa: chemistry,Spermatozoa: cytology,Spermatozoa: drug effects},
 pages = {1553-60},
 volume = {27},
 websites = {http://www.ncbi.nlm.nih.gov/pubmed/18260690},
 month = {7},
 id = {d47f88ce-2e6e-300b-9579-b07d257903e9},
 created = {2014-04-18T08:51:25.000Z},
 file_attached = {false},
 profile_id = {369e8c52-7e84-3c5c-98de-6d14376073ae},
 group_id = {764582e8-5773-3a66-8d6b-9b40e4fb5a88},
 last_modified = {2017-03-14T17:27:14.020Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 citation_key = {Catarino2008},
 abstract = {The present study evaluated the effects of field metal contamination on sperm motility and the RNA/DNA ratio in echinoderms. Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sørfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. The Sørfjord is still one of the most metal-contaminated marine sites in Europe, but even so, populations of A. rubens and E. acutus are able to endure under these conditions.},
 bibtype = {article},
 author = {Catarino, Ana I and Cabral, Henrique N and Peeters, Kris and Pernet, Philippe and Punjabi, Usha and Dubois, Philippe},
 journal = {Environmental toxicology and chemistry / SETAC},
 number = {7}
}

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Populations of Asterias rubens and Echinus acutus that occur naturally along a contamination gradient of sediments by cadmium, copper, lead, and zinc in a Norwegian fjord (the Sørfjord) were studied. Sperm motility, a measure of sperm quality, was quantified using a computer-assisted sperm analysis system. The RNA/DNA ratio, a measure of protein synthesis, was assessed by a one-dye (ethidium bromide)/one-enzyme (RNase), 96-well microplate fluorometric assay. Although both species accumulate metals at high concentrations, neither sperm motility parameters in A. rubens nor the RNA/DNA ratio in both species were affected. 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