Dense Genetic Linkage Maps of Three Populus Species (Populus Deltoides, P. Nigra and P. Trichocarpa) Based on AFLP and Microsatellite Markers. Cervera, M., Storme, V., Ivens, B., Gusmão, J., Liu, B. H., Hostyn, V., Van Slycken, J., Van Montagu, M., & Boerjan, W. 158(2):787–809.
Dense Genetic Linkage Maps of Three Populus Species (Populus Deltoides, P. Nigra and P. Trichocarpa) Based on AFLP and Microsatellite Markers [link]Paper  abstract   bibtex   
Populus deltoides, P. nigra, and P. trichocarpa are the most important species for poplar breeding programs worldwide. In addition, Populus has become a model for fundamental research on trees. Linkage maps were constructed for these three species by analyzing progeny of two controlled crosses sharing the same female parent, Populus deltoides cv. S9-2 × P. nigra cv. Ghoy and P. deltoides cv. S9-2 × P. trichocarpa cv. V24. The two-way pseudotestcross mapping strategy was used to construct the maps. Amplified fragment length polymorphism (AFLP) markers that segregated 1:1 were used to form the four parental maps. Microsatellites and sequence-tagged sites were used to align homoeologous groups between the maps and to merge linkage groups within the individual maps. Linkage analysis and alignment of the homoeologous groups resulted in 566 markers distributed over 19 groups for P. deltoides covering 86\,% of the genome, 339 markers distributed over 19 groups for P. trichocarpa covering 73\,%, and 369 markers distributed over 28 groups for P. nigra covering 61\,%. Several tests for randomness showed that the AFLP markers were randomly distributed over the genome.
@article{cerveraDenseGeneticLinkage2001,
  title = {Dense {{Genetic Linkage Maps}} of {{Three Populus Species}} ({{Populus}} Deltoides, {{P}}. Nigra and {{P}}. Trichocarpa) {{Based}} on {{AFLP}} and {{Microsatellite Markers}}},
  author = {Cervera, Maria-Teresa and Storme, Véronique and Ivens, Bart and Gusmão, Jaqueline and Liu, Ben H. and Hostyn, Vanessa and Van Slycken, Jos and Van Montagu, Marc and Boerjan, Wout},
  date = {2001},
  journaltitle = {Genetics},
  volume = {158},
  pages = {787--809},
  url = {http://www.genetics.org/content/158/2/787.abstract},
  abstract = {Populus deltoides, P. nigra, and P. trichocarpa are the most important species for poplar breeding programs worldwide. In addition, Populus has become a model for fundamental research on trees. Linkage maps were constructed for these three species by analyzing progeny of two controlled crosses sharing the same female parent, Populus deltoides cv. S9-2 × P. nigra cv. Ghoy and P. deltoides cv. S9-2 × P. trichocarpa cv. V24. The two-way pseudotestcross mapping strategy was used to construct the maps. Amplified fragment length polymorphism (AFLP) markers that segregated 1:1 were used to form the four parental maps. Microsatellites and sequence-tagged sites were used to align homoeologous groups between the maps and to merge linkage groups within the individual maps. Linkage analysis and alignment of the homoeologous groups resulted in 566 markers distributed over 19 groups for P. deltoides covering 86\,\% of the genome, 339 markers distributed over 19 groups for P. trichocarpa covering 73\,\%, and 369 markers distributed over 28 groups for P. nigra covering 61\,\%. Several tests for randomness showed that the AFLP markers were randomly distributed over the genome.},
  keywords = {*imported-from-citeulike-INRMM,~INRMM-MiD:c-13523088,populus-nigra,populus-tremuloides,populus-trichocarpa,tree-breeding},
  number = {2}
}

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