{"_id":"9uRtPSoNHwZovwZkC","bibbaseid":"cesbron-royer-guitton-sydor-lebizec-dervillypinel-optimizationoffecalsamplepreparationforuntargetedlchrmsbasedmetabolomics","downloads":0,"creationDate":"2019-03-29T15:39:59.125Z","title":"Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics","author_short":["Cesbron, N.","Royer, A.","Guitton, Y.","Sydor, A.","Le Bizec, B.","Dervilly-Pinel, G."],"year":null,"bibtype":"article","biburl":"https://yguitton.github.io/biblio/mylibrary.bib","bibdata":{"bibtype":"article","type":"article","title":"Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics","volume":"13","issn":"1573-3882, 1573-3890","url":"http://link.springer.com/10.1007/s11306-017-1233-8","doi":"10.1007/s11306-017-1233-8","abstract":"Introduction Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites. Objectives In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting. Methods The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces. Results A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed. Conclusion The workflow generated repeatable and informative fingerprints for robust metabolome characterization.","number":"8","journaltitle":"Metabolomics","author":[{"propositions":[],"lastnames":["Cesbron"],"firstnames":["N."],"suffixes":[]},{"propositions":[],"lastnames":["Royer"],"firstnames":["A.-L."],"suffixes":[]},{"propositions":[],"lastnames":["Guitton"],"firstnames":["Y."],"suffixes":[]},{"propositions":[],"lastnames":["Sydor"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Le","Bizec"],"firstnames":["B."],"suffixes":[]},{"propositions":[],"lastnames":["Dervilly-Pinel"],"firstnames":["G."],"suffixes":[]}],"urldate":"2019-03-29","date":"2017-08","langid":"english","file":"Cesbron et al. - 2017 - Optimization of fecal sample preparation for untar.pdf:C\\:\\\\Users\\\\ygu\\\\Documents\\¶CPOR066_YGU\\\\YGU\\\\Zotero\\\\storage\\\\EJIIIMFH\\\\Cesbron et al. - 2017 - Optimization of fecal sample preparation for untar.pdf:application/pdf","bibtex":"@article{cesbron_optimization_2017,\n\ttitle = {Optimization of fecal sample preparation for untargeted {LC}-{HRMS} based metabolomics},\n\tvolume = {13},\n\tissn = {1573-3882, 1573-3890},\n\turl = {http://link.springer.com/10.1007/s11306-017-1233-8},\n\tdoi = {10.1007/s11306-017-1233-8},\n\tabstract = {Introduction Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites.\nObjectives In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted {LC}-{HRMS} fingerprinting.\nMethods The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces.\nResults A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 {kDa}) was developed.\nConclusion The workflow generated repeatable and informative fingerprints for robust metabolome characterization.},\n\tnumber = {8},\n\tjournaltitle = {Metabolomics},\n\tauthor = {Cesbron, N. and Royer, A.-L. and Guitton, Y. and Sydor, A. and Le Bizec, B. and Dervilly-Pinel, G.},\n\turldate = {2019-03-29},\n\tdate = {2017-08},\n\tlangid = {english},\n\tfile = {Cesbron et al. - 2017 - Optimization of fecal sample preparation for untar.pdf:C\\:\\\\Users\\\\ygu\\\\Documents\\\\PCPOR066_YGU\\\\YGU\\\\Zotero\\\\storage\\\\EJIIIMFH\\\\Cesbron et al. - 2017 - Optimization of fecal sample preparation for untar.pdf:application/pdf}\n}\n\n","author_short":["Cesbron, N.","Royer, A.","Guitton, Y.","Sydor, A.","Le Bizec, B.","Dervilly-Pinel, G."],"key":"cesbron_optimization_2017","id":"cesbron_optimization_2017","bibbaseid":"cesbron-royer-guitton-sydor-lebizec-dervillypinel-optimizationoffecalsamplepreparationforuntargetedlchrmsbasedmetabolomics","role":"author","urls":{"Paper":"http://link.springer.com/10.1007/s11306-017-1233-8"},"metadata":{"authorlinks":{}},"html":""},"search_terms":["optimization","fecal","sample","preparation","untargeted","hrms","based","metabolomics","cesbron","royer","guitton","sydor","le bizec","dervilly-pinel"],"keywords":[],"authorIDs":[],"dataSources":["npZbB8XqBLCP4Micr"]}