Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics. Cesbron, N., Royer, A., Guitton, Y., Sydor, A., Le Bizec, B., & Dervilly-Pinel, G.
Optimization of fecal sample preparation for untargeted LC-HRMS based metabolomics [link]Paper  doi  abstract   bibtex   
Introduction  Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites. Objectives  In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted LC-HRMS fingerprinting. Methods  The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces. Results  A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 kDa) was developed. Conclusion The workflow generated repeatable and informative fingerprints for robust metabolome characterization.
@article{cesbron_optimization_2017,
	title = {Optimization of fecal sample preparation for untargeted {LC}-{HRMS} based metabolomics},
	volume = {13},
	issn = {1573-3882, 1573-3890},
	url = {http://link.springer.com/10.1007/s11306-017-1233-8},
	doi = {10.1007/s11306-017-1233-8},
	abstract = {Introduction  Collecting feces is easy. It offers direct outcome to endogenous and microbial metabolites.
Objectives  In a context of lack of consensus about fecal sample preparation, especially in animal species, we developed a robust protocol allowing untargeted {LC}-{HRMS} fingerprinting.
Methods  The conditions of extraction (quantity, preparation, solvents, dilutions) were investigated in bovine feces.
Results  A rapid and simple protocol involving feces extraction with methanol (1/3, M/V) followed by centrifugation and a step filtration (10 {kDa}) was developed.
Conclusion The workflow generated repeatable and informative fingerprints for robust metabolome characterization.},
	number = {8},
	journaltitle = {Metabolomics},
	author = {Cesbron, N. and Royer, A.-L. and Guitton, Y. and Sydor, A. and Le Bizec, B. and Dervilly-Pinel, G.},
	urldate = {2019-03-29},
	date = {2017-08},
	langid = {english},
	file = {Cesbron et al. - 2017 - Optimization of fecal sample preparation for untar.pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\EJIIIMFH\\Cesbron et al. - 2017 - Optimization of fecal sample preparation for untar.pdf:application/pdf}
}

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