Determination of conifer age biomarker DAL1 interactome using Y2H-seq. Chen, X., Zhu, Q., Nie, Y., Han, F., Li, Y., Wu, H. X., & Niu, S. Forestry Research, 1(1):1–12, July, 2021.
Determination of conifer age biomarker DAL1 interactome using Y2H-seq [link]Paper  doi  abstract   bibtex   2 downloads  
Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms. The regulation of ageing pathways appears to differ between gymnosperms and angiosperms. We previously identified DAL1 as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines. Therefore, elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development. Large-scale yeast two-hybrid (Y2H) analysis followed by next-generation high-throughput sequencing (Y2H-seq) allowed us to identify 135 PtDAL1 interacting proteins in Pinus tabuliformis. Our study found that PtDAL1 interacting proteins showed an ageing-related module, with sophisticated interacting networks composed of transcription factors (TFs), transcriptional regulators (TRs), and kinases. These interacting proteins are produced in response to a variety of phytohormones and environmental signals, and are likely involved in wood formation, needle development, oleoresin terpenoids biosynthesis, and reproductive development. In this study, we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.
@article{chen_determination_2021,
	title = {Determination of conifer age biomarker {DAL1} interactome using {Y2H}-seq},
	volume = {1},
	copyright = {2021 The Author(s)},
	issn = {2767-3812},
	url = {http://www.maxapress.com/rticle/doi/10.48130/FR-2021-0012},
	doi = {10/gnj3pg},
	abstract = {Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms. The regulation of ageing pathways appears to differ between gymnosperms and angiosperms. We previously identified \textit{DAL1} as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines. Therefore, elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development. Large-scale yeast two-hybrid (Y2H) analysis followed by next-generation high-throughput sequencing (Y2H-seq) allowed us to identify 135 PtDAL1 interacting proteins in \textit{Pinus tabuliformis}. Our study found that PtDAL1 interacting proteins showed an ageing-related module, with sophisticated interacting networks composed of transcription factors (TFs), transcriptional regulators (TRs), and kinases. These interacting proteins are produced in response to a variety of phytohormones and environmental signals, and are likely involved in wood formation, needle development, oleoresin terpenoids biosynthesis, and reproductive development. In this study, we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.},
	language = {en},
	number = {1},
	urldate = {2021-11-22},
	journal = {Forestry Research},
	author = {Chen, Xi and Zhu, Qianya and Nie, Yumeng and Han, Fangxu and Li, Yue and Wu, Harry X. and Niu, Shihui},
	month = jul,
	year = {2021},
	pages = {1--12},
}

Downloads: 2