@article{chen_determination_2021,
	title = {Determination of conifer age biomarker {DAL1} interactome using {Y2H}-seq},
	volume = {1},
	copyright = {2021 The Author(s)},
	issn = {2767-3812},
	url = {http://www.maxapress.com/rticle/doi/10.48130/FR-2021-0012},
	doi = {10/gnj3pg},
	abstract = {Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms. The regulation of ageing pathways appears to differ between gymnosperms and angiosperms. We previously identified \textit{DAL1} as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines. Therefore, elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development. Large-scale yeast two-hybrid (Y2H) analysis followed by next-generation high-throughput sequencing (Y2H-seq) allowed us to identify 135 PtDAL1 interacting proteins in \textit{Pinus tabuliformis}. Our study found that PtDAL1 interacting proteins showed an ageing-related module, with sophisticated interacting networks composed of transcription factors (TFs), transcriptional regulators (TRs), and kinases. These interacting proteins are produced in response to a variety of phytohormones and environmental signals, and are likely involved in wood formation, needle development, oleoresin terpenoids biosynthesis, and reproductive development. In this study, we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.},
	language = {en},
	number = {1},
	urldate = {2021-11-22},
	journal = {Forestry Research},
	author = {Chen, Xi and Zhu, Qianya and Nie, Yumeng and Han, Fangxu and Li, Yue and Wu, Harry X. and Niu, Shihui},
	month = jul,
	year = {2021},
	pages = {1--12},
}

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Therefore, elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development. Large-scale yeast two-hybrid (Y2H) analysis followed by next-generation high-throughput sequencing (Y2H-seq) allowed us to identify 135 PtDAL1 interacting proteins in <i>Pinus tabuliformis</i>. Our study found that PtDAL1 interacting proteins showed an ageing-related module, with sophisticated interacting networks composed of transcription factors (TFs), transcriptional regulators (TRs), and kinases. These interacting proteins are produced in response to a variety of phytohormones and environmental signals, and are likely involved in wood formation, needle development, oleoresin terpenoids biosynthesis, and reproductive development. In this study, we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.","language":"en","number":"1","urldate":"2021-11-22","journal":"Forestry Research","author":[{"propositions":[],"lastnames":["Chen"],"firstnames":["Xi"],"suffixes":[]},{"propositions":[],"lastnames":["Zhu"],"firstnames":["Qianya"],"suffixes":[]},{"propositions":[],"lastnames":["Nie"],"firstnames":["Yumeng"],"suffixes":[]},{"propositions":[],"lastnames":["Han"],"firstnames":["Fangxu"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Yue"],"suffixes":[]},{"propositions":[],"lastnames":["Wu"],"firstnames":["Harry","X."],"suffixes":[]},{"propositions":[],"lastnames":["Niu"],"firstnames":["Shihui"],"suffixes":[]}],"month":"July","year":"2021","pages":"1–12","bibtex":"@article{chen_determination_2021,\n\ttitle = {Determination of conifer age biomarker {DAL1} interactome using {Y2H}-seq},\n\tvolume = {1},\n\tcopyright = {2021 The Author(s)},\n\tissn = {2767-3812},\n\turl = {http://www.maxapress.com/rticle/doi/10.48130/FR-2021-0012},\n\tdoi = {10/gnj3pg},\n\tabstract = {Age is a sophisticated physiological signal that ensures the sequence of different developmental stages in organisms. The regulation of ageing pathways appears to differ between gymnosperms and angiosperms. We previously identified \\textit{DAL1} as a conserved conifer age biomarker that plays a crucial role in the transition from vegetative to reproductive life-history phases in pines. Therefore, elucidating the specific interaction events related to DAL1 is key to understanding how age drives conifer development. Large-scale yeast two-hybrid (Y2H) analysis followed by next-generation high-throughput sequencing (Y2H-seq) allowed us to identify 135 PtDAL1 interacting proteins in \\textit{Pinus tabuliformis}. Our study found that PtDAL1 interacting proteins showed an ageing-related module, with sophisticated interacting networks composed of transcription factors (TFs), transcriptional regulators (TRs), and kinases. These interacting proteins are produced in response to a variety of phytohormones and environmental signals, and are likely involved in wood formation, needle development, oleoresin terpenoids biosynthesis, and reproductive development. In this study, we propose a novel regulation model of conifer ageing pathways whereby PtDAL1 coordinates different environmental stimuli and interacts with corresponding proteins to regulate appropriate development.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2021-11-22},\n\tjournal = {Forestry Research},\n\tauthor = {Chen, Xi and Zhu, Qianya and Nie, Yumeng and Han, Fangxu and Li, Yue and Wu, Harry X. and Niu, Shihui},\n\tmonth = jul,\n\tyear = {2021},\n\tpages = {1--12},\n}\n\n\n\n","author_short":["Chen, X.","Zhu, Q.","Nie, Y.","Han, F.","Li, Y.","Wu, H. X.","Niu, S."],"key":"chen_determination_2021","id":"chen_determination_2021","bibbaseid":"chen-zhu-nie-han-li-wu-niu-determinationofconiferagebiomarkerdal1interactomeusingy2hseq-2021","role":"author","urls":{"Paper":"http://www.maxapress.com/rticle/doi/10.48130/FR-2021-0012"},"metadata":{"authorlinks":{}},"downloads":2},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["Tu3jPdZyJF3j547xT","9cGcv2t8pRzC92kzs","3zTPPmKj8BiTcpc6C"],"keywords":[],"search_terms":["determination","conifer","age","biomarker","dal1","interactome","using","y2h","seq","chen","zhu","nie","han","li","wu","niu"],"title":"Determination of conifer age biomarker DAL1 interactome using Y2H-seq","year":2021,"downloads":2}