Protocol for the separation of extracellular vesicles by ultracentrifugation from in vitro cell culture models

Protocol for the separation of extracellular vesicles by ultracentrifugation from in vitro cell culture models. Chhoy, P., Brown, C. W., Amante, J. J., & Mercurio, A. M. STAR Protocols, 2(1):100303, March, 2021.

Paper doi abstract bibtex

Extracellular vesicles (EVs) play key roles in transporting key molecular constituents as cargo for extracellular trafﬁcking. While several approaches have been developed to extract EVs from mammalian cells, the speciﬁc method of EV isolation can have a profound effect on membrane integrity and yield. Here, we describe a step-by-step procedure to separate EVs from adherent epithelial cells using differential ultracentrifugation. Separated EVs can be further analyzed by immunoblotting, mass spectrometry, and transmission electron microscopy to derive EV yield and morphology.

@article{chhoy_protocol_2021,
	title = {Protocol for the separation of extracellular vesicles by ultracentrifugation from in vitro cell culture models},
	volume = {2},
	issn = {26661667},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S2666166721000101},
	doi = {10.1016/j.xpro.2021.100303},
	abstract = {Extracellular vesicles (EVs) play key roles in transporting key molecular constituents as cargo for extracellular trafﬁcking. While several approaches have been developed to extract EVs from mammalian cells, the speciﬁc method of EV isolation can have a profound effect on membrane integrity and yield. Here, we describe a step-by-step procedure to separate EVs from adherent epithelial cells using differential ultracentrifugation. Separated EVs can be further analyzed by immunoblotting, mass spectrometry, and transmission electron microscopy to derive EV yield and morphology.},
	language = {en},
	number = {1},
	urldate = {2022-08-12},
	journal = {STAR Protocols},
	author = {Chhoy, Peter and Brown, Caitlin W. and Amante, John J. and Mercurio, Arthur M.},
	month = mar,
	year = {2021},
	pages = {100303},
}

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