Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration. Chicharo, A., Martins, M., Barnsley, L. C., Taouallah, A., Fernandes, J., Silva, B. F. B., Cardoso, S., Dieguez, L., Espina, B., & Freitas, P. P. LAB ON A CHIP, 18(17):2593-2603, SEP 7, 2018. doi abstract bibtex We report the design and characterization of a lateral and vertical hydrodynamic focusing feature for whole cell detection on a miniaturized flow cytometer. The developed system, based on magnetic sensing, incorporates spin valve sensors on the bottom of the microfluidic channels that detect cells labeled with magnetic beads. An adaptable 3D hydrodynamic focusing system was developed that pushes labeled cells towards the bottom of the microchannel, closer to the sensors, allowing increased signal amplitude for cells labeled with magnetic beads and enhanced discrimination of labeled cells. Fluorescence microscopy indicates that the lateral and vertical hydrodynamic focusing effect was adequately implemented, consistent with simulation predictions. The sensitivity of the system to detect labeled cells was improved by at least two-fold. By estimating the coverage of magnetic beads on cells, the signal from labeled cells could be predicted using a mathematical model, which also demonstrated the sensitivity of the signal to the height of the cells relative to the sensor. The system is versatile allowing interchangeable flow rates for cells with different diameters.
@article{ ISI:000443279400009,
Author = {Chicharo, Alexandre and Martins, Marco and Barnsley, Lester C. and
Taouallah, Amal and Fernandes, Joao and Silva, Bruno F. B. and Cardoso,
Susana and Dieguez, Lorena and Espina, Begona and Freitas, Paulo P.},
Title = {{Enhanced magnetic microcytometer with 3D flow focusing for cell
enumeration}},
Journal = {{LAB ON A CHIP}},
Year = {{2018}},
Volume = {{18}},
Number = {{17}},
Pages = {{2593-2603}},
Month = {{SEP 7}},
Abstract = {{We report the design and characterization of a lateral and vertical
hydrodynamic focusing feature for whole cell detection on a miniaturized
flow cytometer. The developed system, based on magnetic sensing,
incorporates spin valve sensors on the bottom of the microfluidic
channels that detect cells labeled with magnetic beads. An adaptable 3D
hydrodynamic focusing system was developed that pushes labeled cells
towards the bottom of the microchannel, closer to the sensors, allowing
increased signal amplitude for cells labeled with magnetic beads and
enhanced discrimination of labeled cells. Fluorescence microscopy
indicates that the lateral and vertical hydrodynamic focusing effect was
adequately implemented, consistent with simulation predictions. The
sensitivity of the system to detect labeled cells was improved by at
least two-fold. By estimating the coverage of magnetic beads on cells,
the signal from labeled cells could be predicted using a mathematical
model, which also demonstrated the sensitivity of the signal to the
height of the cells relative to the sensor. The system is versatile
allowing interchangeable flow rates for cells with different diameters.}},
DOI = {{10.1039/c8lc00486b}},
ISSN = {{1473-0197}},
EISSN = {{1473-0189}},
ResearcherID-Numbers = {{Dieguez, Lorena/E-4676-2011
Silva, Bruno F.B./F-8591-2015
de Freitas, Susana Cardoso/B-6199-2013
Chicharo, Alexandre/T-4781-2017
Freitas, Paulo J.P./B-6164-2013
Espina, Begona/H-1723-2014
}},
ORCID-Numbers = {{Dieguez, Lorena/0000-0003-3695-6963
Silva, Bruno F.B./0000-0002-8997-6311
de Freitas, Susana Cardoso/0000-0001-6913-6529
Chicharo, Alexandre/0000-0002-5788-4316
Freitas, Paulo J.P./0000-0003-0015-1186
Espina, Begona/0000-0002-7645-2834
Martins, Joao Marco/0000-0002-1531-1675
Barnsley, Lester/0000-0002-1341-905X}},
Unique-ID = {{ISI:000443279400009}},
}
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The developed system, based on magnetic sensing, incorporates spin valve sensors on the bottom of the microfluidic channels that detect cells labeled with magnetic beads. An adaptable 3D hydrodynamic focusing system was developed that pushes labeled cells towards the bottom of the microchannel, closer to the sensors, allowing increased signal amplitude for cells labeled with magnetic beads and enhanced discrimination of labeled cells. Fluorescence microscopy indicates that the lateral and vertical hydrodynamic focusing effect was adequately implemented, consistent with simulation predictions. The sensitivity of the system to detect labeled cells was improved by at least two-fold. By estimating the coverage of magnetic beads on cells, the signal from labeled cells could be predicted using a mathematical model, which also demonstrated the sensitivity of the signal to the height of the cells relative to the sensor. 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B. and Cardoso,\n Susana and Dieguez, Lorena and Espina, Begona and Freitas, Paulo P.},\nTitle = {{Enhanced magnetic microcytometer with 3D flow focusing for cell\n enumeration}},\nJournal = {{LAB ON A CHIP}},\nYear = {{2018}},\nVolume = {{18}},\nNumber = {{17}},\nPages = {{2593-2603}},\nMonth = {{SEP 7}},\nAbstract = {{We report the design and characterization of a lateral and vertical\n hydrodynamic focusing feature for whole cell detection on a miniaturized\n flow cytometer. The developed system, based on magnetic sensing,\n incorporates spin valve sensors on the bottom of the microfluidic\n channels that detect cells labeled with magnetic beads. An adaptable 3D\n hydrodynamic focusing system was developed that pushes labeled cells\n towards the bottom of the microchannel, closer to the sensors, allowing\n increased signal amplitude for cells labeled with magnetic beads and\n enhanced discrimination of labeled cells. Fluorescence microscopy\n indicates that the lateral and vertical hydrodynamic focusing effect was\n adequately implemented, consistent with simulation predictions. The\n sensitivity of the system to detect labeled cells was improved by at\n least two-fold. By estimating the coverage of magnetic beads on cells,\n the signal from labeled cells could be predicted using a mathematical\n model, which also demonstrated the sensitivity of the signal to the\n height of the cells relative to the sensor. 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