Monitoring triplex DNA formation with fluorescence resonance energy transfer between a fluorophore-labeled probe and intercalating dyes. Chiou, C., Chen, S., Luo, J., & Chien, Y. Analytical biochemistry, 416(1):1–7, September, 2011. Publisher: Elsevier Inc.![Monitoring triplex DNA formation with fluorescence resonance energy transfer between a fluorophore-labeled probe and intercalating dyes. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Triplex-forming oligonucleotides (TFOs) are sequence-dependent DNA binders that may be useful for DNA targeting and detection. A sensitive and convenient method to monitor triplex formation by a TFO and its target DNA duplex is required for the application of TFO probes. Here we describe a novel design by which triplex formation can be monitored homogeneously without prelabeling the target duplex. The design uses a TFO probe tagged with a fluorophore that undergoes fluorescence resonance energy transfer with fluorescent dyes that intercalate into the target duplex. Through color compensation analysis, the specific emission of the TFO probe reveals the status of the triple helices. We used this method to show that triple helix formation with TFOs is magnesium dependent. We also demonstrated that the TFO probe can be used for detection of sequence variation in melting analysis and for DNA quantitation in real-time polymerase chain reaction.
@article{Chiou2011,
title = {Monitoring triplex {DNA} formation with fluorescence resonance energy transfer between a fluorophore-labeled probe and intercalating dyes.},
volume = {416},
issn = {1096-0309},
url = {http://www.ncbi.nlm.nih.gov/pubmed/21609711},
doi = {10.1016/j.ab.2011.05.002},
abstract = {Triplex-forming oligonucleotides (TFOs) are sequence-dependent DNA binders that may be useful for DNA targeting and detection. A sensitive and convenient method to monitor triplex formation by a TFO and its target DNA duplex is required for the application of TFO probes. Here we describe a novel design by which triplex formation can be monitored homogeneously without prelabeling the target duplex. The design uses a TFO probe tagged with a fluorophore that undergoes fluorescence resonance energy transfer with fluorescent dyes that intercalate into the target duplex. Through color compensation analysis, the specific emission of the TFO probe reveals the status of the triple helices. We used this method to show that triple helix formation with TFOs is magnesium dependent. We also demonstrated that the TFO probe can be used for detection of sequence variation in melting analysis and for DNA quantitation in real-time polymerase chain reaction.},
number = {1},
journal = {Analytical biochemistry},
author = {Chiou, Chiuan-Chian and Chen, Shiau-Wen and Luo, Ji-Dung and Chien, Yu-Tzu},
month = sep,
year = {2011},
pmid = {21609711},
note = {Publisher: Elsevier Inc.},
keywords = {\#nosource, DNA, DNA: analysis, DNA: chemistry, DNA: genetics, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Fluorescent Dyes: analysis, Fluorescent Dyes: chemical synthesis, Fluorescent Dyes: chemistry, Intercalating Agents, Intercalating Agents: chemistry, Nucleic Acid Denaturation, Oligonucleotides, Oligonucleotides: analysis, Oligonucleotides: chemical synthesis, Oligonucleotides: chemistry, Plasmids, Plasmids: genetics, Reverse Transcriptase Polymerase Chain Reaction, Temperature},
pages = {1--7},
}
Downloads: 0
{"_id":"3RE6nkdzKAvuQTTYb","bibbaseid":"chiou-chen-luo-chien-monitoringtriplexdnaformationwithfluorescenceresonanceenergytransferbetweenafluorophorelabeledprobeandintercalatingdyes-2011","author_short":["Chiou, C.","Chen, S.","Luo, J.","Chien, Y."],"bibdata":{"bibtype":"article","type":"article","title":"Monitoring triplex DNA formation with fluorescence resonance energy transfer between a fluorophore-labeled probe and intercalating dyes.","volume":"416","issn":"1096-0309","url":"http://www.ncbi.nlm.nih.gov/pubmed/21609711","doi":"10.1016/j.ab.2011.05.002","abstract":"Triplex-forming oligonucleotides (TFOs) are sequence-dependent DNA binders that may be useful for DNA targeting and detection. A sensitive and convenient method to monitor triplex formation by a TFO and its target DNA duplex is required for the application of TFO probes. Here we describe a novel design by which triplex formation can be monitored homogeneously without prelabeling the target duplex. The design uses a TFO probe tagged with a fluorophore that undergoes fluorescence resonance energy transfer with fluorescent dyes that intercalate into the target duplex. Through color compensation analysis, the specific emission of the TFO probe reveals the status of the triple helices. We used this method to show that triple helix formation with TFOs is magnesium dependent. We also demonstrated that the TFO probe can be used for detection of sequence variation in melting analysis and for DNA quantitation in real-time polymerase chain reaction.","number":"1","journal":"Analytical biochemistry","author":[{"propositions":[],"lastnames":["Chiou"],"firstnames":["Chiuan-Chian"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["Shiau-Wen"],"suffixes":[]},{"propositions":[],"lastnames":["Luo"],"firstnames":["Ji-Dung"],"suffixes":[]},{"propositions":[],"lastnames":["Chien"],"firstnames":["Yu-Tzu"],"suffixes":[]}],"month":"September","year":"2011","pmid":"21609711","note":"Publisher: Elsevier Inc.","keywords":"#nosource, DNA, DNA: analysis, DNA: chemistry, DNA: genetics, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Fluorescent Dyes: analysis, Fluorescent Dyes: chemical synthesis, Fluorescent Dyes: chemistry, Intercalating Agents, Intercalating Agents: chemistry, Nucleic Acid Denaturation, Oligonucleotides, Oligonucleotides: analysis, Oligonucleotides: chemical synthesis, Oligonucleotides: chemistry, Plasmids, Plasmids: genetics, Reverse Transcriptase Polymerase Chain Reaction, Temperature","pages":"1–7","bibtex":"@article{Chiou2011,\n\ttitle = {Monitoring triplex {DNA} formation with fluorescence resonance energy transfer between a fluorophore-labeled probe and intercalating dyes.},\n\tvolume = {416},\n\tissn = {1096-0309},\n\turl = {http://www.ncbi.nlm.nih.gov/pubmed/21609711},\n\tdoi = {10.1016/j.ab.2011.05.002},\n\tabstract = {Triplex-forming oligonucleotides (TFOs) are sequence-dependent DNA binders that may be useful for DNA targeting and detection. A sensitive and convenient method to monitor triplex formation by a TFO and its target DNA duplex is required for the application of TFO probes. Here we describe a novel design by which triplex formation can be monitored homogeneously without prelabeling the target duplex. The design uses a TFO probe tagged with a fluorophore that undergoes fluorescence resonance energy transfer with fluorescent dyes that intercalate into the target duplex. Through color compensation analysis, the specific emission of the TFO probe reveals the status of the triple helices. We used this method to show that triple helix formation with TFOs is magnesium dependent. We also demonstrated that the TFO probe can be used for detection of sequence variation in melting analysis and for DNA quantitation in real-time polymerase chain reaction.},\n\tnumber = {1},\n\tjournal = {Analytical biochemistry},\n\tauthor = {Chiou, Chiuan-Chian and Chen, Shiau-Wen and Luo, Ji-Dung and Chien, Yu-Tzu},\n\tmonth = sep,\n\tyear = {2011},\n\tpmid = {21609711},\n\tnote = {Publisher: Elsevier Inc.},\n\tkeywords = {\\#nosource, DNA, DNA: analysis, DNA: chemistry, DNA: genetics, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, Fluorescent Dyes: analysis, Fluorescent Dyes: chemical synthesis, Fluorescent Dyes: chemistry, Intercalating Agents, Intercalating Agents: chemistry, Nucleic Acid Denaturation, Oligonucleotides, Oligonucleotides: analysis, Oligonucleotides: chemical synthesis, Oligonucleotides: chemistry, Plasmids, Plasmids: genetics, Reverse Transcriptase Polymerase Chain Reaction, Temperature},\n\tpages = {1--7},\n}\n\n","author_short":["Chiou, C.","Chen, S.","Luo, J.","Chien, Y."],"key":"Chiou2011","id":"Chiou2011","bibbaseid":"chiou-chen-luo-chien-monitoringtriplexdnaformationwithfluorescenceresonanceenergytransferbetweenafluorophorelabeledprobeandintercalatingdyes-2011","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/pubmed/21609711"},"keyword":["#nosource","DNA","DNA: analysis","DNA: chemistry","DNA: genetics","Fluorescence Resonance Energy Transfer","Fluorescent Dyes","Fluorescent Dyes: analysis","Fluorescent Dyes: chemical synthesis","Fluorescent Dyes: chemistry","Intercalating Agents","Intercalating Agents: chemistry","Nucleic Acid Denaturation","Oligonucleotides","Oligonucleotides: analysis","Oligonucleotides: chemical synthesis","Oligonucleotides: chemistry","Plasmids","Plasmids: genetics","Reverse Transcriptase Polymerase Chain Reaction","Temperature"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/eric.larG4","dataSources":["4i5C7S78DvJNsaHyg","5L2zM5wNE5CBYNuea"],"keywords":["#nosource","dna","dna: analysis","dna: chemistry","dna: genetics","fluorescence resonance energy transfer","fluorescent dyes","fluorescent dyes: analysis","fluorescent dyes: chemical synthesis","fluorescent dyes: chemistry","intercalating agents","intercalating agents: chemistry","nucleic acid denaturation","oligonucleotides","oligonucleotides: analysis","oligonucleotides: chemical synthesis","oligonucleotides: chemistry","plasmids","plasmids: genetics","reverse transcriptase polymerase chain reaction","temperature"],"search_terms":["monitoring","triplex","dna","formation","fluorescence","resonance","energy","transfer","between","fluorophore","labeled","probe","intercalating","dyes","chiou","chen","luo","chien"],"title":"Monitoring triplex DNA formation with fluorescence resonance energy transfer between a fluorophore-labeled probe and intercalating dyes.","year":2011}