@article{ ISI:000455067800006,
Author = {Chiriaco, Maria Serena and Parlangeli, Ilaria and Sirsi, Fausto and
   Poltronieri, Palmiro and Primiceri, Elisabetta},
Title = {{Impedance Sensing Platform for Detection of the Food Pathogen Listeria
   monocytogenes}},
Journal = {{ELECTRONICS}},
Year = {{2018}},
Volume = {{7}},
Number = {{12}},
Month = {{DEC}},
Abstract = {{A great improvement in food safety and quality controls worldwide has
   been achieved through the development of biosensing platforms. Foodborne
   pathogens continue to cause serious outbreaks, due to the ingestion of
   contaminated food. The development of new, sensitive, portable,
   high-throughput, and automated platforms is a primary objective to allow
   detection of pathogens and their toxins in foods. Listeria monocytogenes
   is one common foodborne pathogen. Major outbreaks of listeriosis have
   been caused by a variety of foods, including milk, soft cheeses, meat,
   fermented sausages, poultry, seafood and vegetable products. Due to its
   high sensitivity and easy setup, electrochemical impedance spectroscopy
   (EIS) has been extensively applied for biosensor fabrication and in
   particular in the field of microbiology as a mean to detect and quantify
   foodborne bacteria. Here we describe a miniaturized, portable EIS
   platform consisting of a microfluidic device with EIS sensors for the
   detection of L. monocytogenes in milk samples, connected to a portable
   impedance analyzer for on-field application in clinical and food
   diagnostics, but also for biosecurity purposes. To achieve this goal
   microelectrodes were functionalized with antibodies specific for L.
   monocytogenes. The binding and detection of L. monocytogenes was
   achieved in the range 2.2 x 10(3) cfu/mL to 1 x 10(2) with a Limit of
   Detection (LoD) of 5.5 cfu/mL.}},
DOI = {{10.3390/electronics7120347}},
Article-Number = {{347}},
ISSN = {{2079-9292}},
ResearcherID-Numbers = {{Poltronieri, Palmiro/B-4813-2015
   Chiriaco, Maria Serena/K-6082-2016
   primiceri, elisabetta/P-8842-2015}},
ORCID-Numbers = {{Poltronieri, Palmiro/0000-0002-5404-9411
   Chiriaco, Maria Serena/0000-0002-7195-267X
   primiceri, elisabetta/0000-0002-4196-3538}},
Unique-ID = {{ISI:000455067800006}},
}

{"_id":"uNfPadLnSrBjSxxjs","bibbaseid":"chiriaco-parlangeli-sirsi-poltronieri-primiceri-impedancesensingplatformfordetectionofthefoodpathogenlisteriamonocytogenes-2018","authorIDs":[],"author_short":["Chiriaco, M. S.","Parlangeli, I.","Sirsi, F.","Poltronieri, P.","Primiceri, E."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Chiriaco"],"firstnames":["Maria","Serena"],"suffixes":[]},{"propositions":[],"lastnames":["Parlangeli"],"firstnames":["Ilaria"],"suffixes":[]},{"propositions":[],"lastnames":["Sirsi"],"firstnames":["Fausto"],"suffixes":[]},{"propositions":[],"lastnames":["Poltronieri"],"firstnames":["Palmiro"],"suffixes":[]},{"propositions":[],"lastnames":["Primiceri"],"firstnames":["Elisabetta"],"suffixes":[]}],"title":"Impedance Sensing Platform for Detection of the Food Pathogen Listeria monocytogenes","journal":"ELECTRONICS","year":"2018","volume":"7","number":"12","month":"DEC","abstract":"A great improvement in food safety and quality controls worldwide has been achieved through the development of biosensing platforms. Foodborne pathogens continue to cause serious outbreaks, due to the ingestion of contaminated food. The development of new, sensitive, portable, high-throughput, and automated platforms is a primary objective to allow detection of pathogens and their toxins in foods. Listeria monocytogenes is one common foodborne pathogen. Major outbreaks of listeriosis have been caused by a variety of foods, including milk, soft cheeses, meat, fermented sausages, poultry, seafood and vegetable products. Due to its high sensitivity and easy setup, electrochemical impedance spectroscopy (EIS) has been extensively applied for biosensor fabrication and in particular in the field of microbiology as a mean to detect and quantify foodborne bacteria. Here we describe a miniaturized, portable EIS platform consisting of a microfluidic device with EIS sensors for the detection of L. monocytogenes in milk samples, connected to a portable impedance analyzer for on-field application in clinical and food diagnostics, but also for biosecurity purposes. To achieve this goal microelectrodes were functionalized with antibodies specific for L. monocytogenes. The binding and detection of L. monocytogenes was achieved in the range 2.2 x 10(3) cfu/mL to 1 x 10(2) with a Limit of Detection (LoD) of 5.5 cfu/mL.","doi":"10.3390/electronics7120347","article-number":"347","issn":"2079-9292","researcherid-numbers":"Poltronieri, Palmiro/B-4813-2015 Chiriaco, Maria Serena/K-6082-2016 primiceri, elisabetta/P-8842-2015","orcid-numbers":"Poltronieri, Palmiro/0000-0002-5404-9411 Chiriaco, Maria Serena/0000-0002-7195-267X primiceri, elisabetta/0000-0002-4196-3538","unique-id":"ISI:000455067800006","bibtex":"@article{ ISI:000455067800006,\nAuthor = {Chiriaco, Maria Serena and Parlangeli, Ilaria and Sirsi, Fausto and\n Poltronieri, Palmiro and Primiceri, Elisabetta},\nTitle = {{Impedance Sensing Platform for Detection of the Food Pathogen Listeria\n monocytogenes}},\nJournal = {{ELECTRONICS}},\nYear = {{2018}},\nVolume = {{7}},\nNumber = {{12}},\nMonth = {{DEC}},\nAbstract = {{A great improvement in food safety and quality controls worldwide has\n been achieved through the development of biosensing platforms. Foodborne\n pathogens continue to cause serious outbreaks, due to the ingestion of\n contaminated food. The development of new, sensitive, portable,\n high-throughput, and automated platforms is a primary objective to allow\n detection of pathogens and their toxins in foods. Listeria monocytogenes\n is one common foodborne pathogen. Major outbreaks of listeriosis have\n been caused by a variety of foods, including milk, soft cheeses, meat,\n fermented sausages, poultry, seafood and vegetable products. Due to its\n high sensitivity and easy setup, electrochemical impedance spectroscopy\n (EIS) has been extensively applied for biosensor fabrication and in\n particular in the field of microbiology as a mean to detect and quantify\n foodborne bacteria. Here we describe a miniaturized, portable EIS\n platform consisting of a microfluidic device with EIS sensors for the\n detection of L. monocytogenes in milk samples, connected to a portable\n impedance analyzer for on-field application in clinical and food\n diagnostics, but also for biosecurity purposes. To achieve this goal\n microelectrodes were functionalized with antibodies specific for L.\n monocytogenes. The binding and detection of L. monocytogenes was\n achieved in the range 2.2 x 10(3) cfu/mL to 1 x 10(2) with a Limit of\n Detection (LoD) of 5.5 cfu/mL.}},\nDOI = {{10.3390/electronics7120347}},\nArticle-Number = {{347}},\nISSN = {{2079-9292}},\nResearcherID-Numbers = {{Poltronieri, Palmiro/B-4813-2015\n Chiriaco, Maria Serena/K-6082-2016\n primiceri, elisabetta/P-8842-2015}},\nORCID-Numbers = {{Poltronieri, Palmiro/0000-0002-5404-9411\n Chiriaco, Maria Serena/0000-0002-7195-267X\n primiceri, elisabetta/0000-0002-4196-3538}},\nUnique-ID = {{ISI:000455067800006}},\n}\n\n","author_short":["Chiriaco, M. 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