@article{
 title = {Simple and efficient plastid transformation system for the liverwort Marchantia polymorpha L. suspension-culture cells.},
 type = {article},
 year = {2007},
 pages = {41-49},
 volume = {16},
 websites = {http://www.springerlink.com/index/X83KVJ67874L172R.pdf},
 publisher = {Springer},
 institution = {Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kyoto, 606-8502, Japan.},
 id = {8f9809a9-15a5-3cda-80ee-0ac9dbe86432},
 created = {2011-05-20T17:26:07.000Z},
 file_attached = {false},
 profile_id = {b6c31fe8-61c6-3818-89a8-62873f3171f3},
 group_id = {7bdcaa0c-1528-351f-a09a-f8da52223946},
 last_modified = {2011-05-20T17:26:07.000Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
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 abstract = {We have established a simple and efficient plastid transformation system for liverwort, Marchantia polymorpha L., suspension-culture cells, which are homogenous, chloroplast-rich and rapidly growing. Plasmid pCS31 was constructed to integrate an aadA expression cassette for spectinomycin-resistance into the trnI-trnA intergenic region of the liverwort plastid DNA by homologous recombination. Liverwort suspension-culture cells were bombarded with pCS31-coated gold projectiles and selected on a medium containing spectinomycin. Plastid transformants were reproducibly isolated from the obtained spectinomycin-resistant calli. Selection on a sucrose-free medium greatly improved the efficiency of selection of plastid transformants. Homoplasmic plastid transformant lines were established by successive subculturing for 14 weeks or longer on the spectinomycin-containing medium. The plastid transformation system of liverwort suspension-culture cells should facilitate the investigation of the fundamental genetic systems of plastid DNA, such as replication.},
 bibtype = {article},
 author = {Chiyoda, Shota and Linley, Philip J and Yamato, Katsuyuki T and Fukuzawa, Hideya and Yokota, Akiho and Kohchi, Takayuki},
 journal = {Transgenic Research},
 number = {1}
}

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