The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells: Application of NB-hNME1 as a Human NME1 Suppressor. Cho, J. H., Ju, W. S., Seo, S. Y., Kim, B. H., Kim, J., Kim, J., Park, S. J., & Choo, Y. International Journal of Molecular Sciences, 22(22):12194, November, 2021.
Paper doi abstract bibtex 2 downloads This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.
@article{cho_potential_2021,
title = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}: {Application} of {NB}-{hNME1} as a {Human} {NME1} {Suppressor}},
volume = {22},
issn = {1422-0067},
shorttitle = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}},
url = {https://www.mdpi.com/1422-0067/22/22/12194},
doi = {10.3390/ijms222212194},
abstract = {This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.},
language = {eng},
number = {22},
journal = {International Journal of Molecular Sciences},
author = {Cho, Jin Hyoung and Ju, Won Seok and Seo, Sang Young and Kim, Bo Hyun and Kim, Ji-Su and Kim, Jong-Geol and Park, Soon Ju and Choo, Young-Kug},
month = nov,
year = {2021},
pmid = {34830075},
keywords = {Application - Antibody Validation / Epitope Mapping, PEPperCHIP - Customized - Linear},
pages = {12194},
}
Downloads: 2
{"_id":"ou7sdr7toNt7szkDJ","bibbaseid":"cho-ju-seo-kim-kim-kim-park-choo-thepotentialroleofhumannme1inneuronaldifferentiationofporcinemesenchymalstemcellsapplicationofnbhnme1asahumannme1suppressor-2021","author_short":["Cho, J. H.","Ju, W. S.","Seo, S. Y.","Kim, B. H.","Kim, J.","Kim, J.","Park, S. J.","Choo, Y."],"bibdata":{"bibtype":"article","type":"article","title":"The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells: Application of NB-hNME1 as a Human NME1 Suppressor","volume":"22","issn":"1422-0067","shorttitle":"The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells","url":"https://www.mdpi.com/1422-0067/22/22/12194","doi":"10.3390/ijms222212194","abstract":"This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.","language":"eng","number":"22","journal":"International Journal of Molecular Sciences","author":[{"propositions":[],"lastnames":["Cho"],"firstnames":["Jin","Hyoung"],"suffixes":[]},{"propositions":[],"lastnames":["Ju"],"firstnames":["Won","Seok"],"suffixes":[]},{"propositions":[],"lastnames":["Seo"],"firstnames":["Sang","Young"],"suffixes":[]},{"propositions":[],"lastnames":["Kim"],"firstnames":["Bo","Hyun"],"suffixes":[]},{"propositions":[],"lastnames":["Kim"],"firstnames":["Ji-Su"],"suffixes":[]},{"propositions":[],"lastnames":["Kim"],"firstnames":["Jong-Geol"],"suffixes":[]},{"propositions":[],"lastnames":["Park"],"firstnames":["Soon","Ju"],"suffixes":[]},{"propositions":[],"lastnames":["Choo"],"firstnames":["Young-Kug"],"suffixes":[]}],"month":"November","year":"2021","pmid":"34830075","keywords":"Application - Antibody Validation / Epitope Mapping, PEPperCHIP - Customized - Linear","pages":"12194","bibtex":"@article{cho_potential_2021,\n\ttitle = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}: {Application} of {NB}-{hNME1} as a {Human} {NME1} {Suppressor}},\n\tvolume = {22},\n\tissn = {1422-0067},\n\tshorttitle = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}},\n\turl = {https://www.mdpi.com/1422-0067/22/22/12194},\n\tdoi = {10.3390/ijms222212194},\n\tabstract = {This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.},\n\tlanguage = {eng},\n\tnumber = {22},\n\tjournal = {International Journal of Molecular Sciences},\n\tauthor = {Cho, Jin Hyoung and Ju, Won Seok and Seo, Sang Young and Kim, Bo Hyun and Kim, Ji-Su and Kim, Jong-Geol and Park, Soon Ju and Choo, Young-Kug},\n\tmonth = nov,\n\tyear = {2021},\n\tpmid = {34830075},\n\tkeywords = {Application - Antibody Validation / Epitope Mapping, PEPperCHIP - Customized - Linear},\n\tpages = {12194},\n}\n\n","author_short":["Cho, J. H.","Ju, W. S.","Seo, S. Y.","Kim, B. H.","Kim, J.","Kim, J.","Park, S. J.","Choo, Y."],"key":"cho_potential_2021","id":"cho_potential_2021","bibbaseid":"cho-ju-seo-kim-kim-kim-park-choo-thepotentialroleofhumannme1inneuronaldifferentiationofporcinemesenchymalstemcellsapplicationofnbhnme1asahumannme1suppressor-2021","role":"author","urls":{"Paper":"https://www.mdpi.com/1422-0067/22/22/12194"},"keyword":["Application - Antibody Validation / Epitope Mapping","PEPperCHIP - Customized - Linear"],"metadata":{"authorlinks":{}},"downloads":2},"bibtype":"article","biburl":"https://bibbase.org/zotero/PEPperPAPER","dataSources":["uik5uKfH7ojkHRgZZ","QsdwieyNM5tTaC5Xz","idrrfXaW5AQj5dNMY","NvXC6TpyECRTzBpu8"],"keywords":["application - antibody validation / epitope mapping","pepperchip - customized - linear"],"search_terms":["potential","role","human","nme1","neuronal","differentiation","porcine","mesenchymal","stem","cells","application","hnme1","human","nme1","suppressor","cho","ju","seo","kim","kim","kim","park","choo"],"title":"The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells: Application of NB-hNME1 as a Human NME1 Suppressor","year":2021,"downloads":2}