@article{cho_potential_2021,
	title = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}: {Application} of {NB}-{hNME1} as a {Human} {NME1} {Suppressor}},
	volume = {22},
	issn = {1422-0067},
	shorttitle = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}},
	url = {https://www.mdpi.com/1422-0067/22/22/12194},
	doi = {10.3390/ijms222212194},
	abstract = {This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.},
	language = {eng},
	number = {22},
	journal = {International Journal of Molecular Sciences},
	author = {Cho, Jin Hyoung and Ju, Won Seok and Seo, Sang Young and Kim, Bo Hyun and Kim, Ji-Su and Kim, Jong-Geol and Park, Soon Ju and Choo, Young-Kug},
	month = nov,
	year = {2021},
	pmid = {34830075},
	keywords = {Application - Antibody Validation / Epitope Mapping, PEPperCHIP - Customized - Linear},
	pages = {12194},
}

{"_id":"ou7sdr7toNt7szkDJ","bibbaseid":"cho-ju-seo-kim-kim-kim-park-choo-thepotentialroleofhumannme1inneuronaldifferentiationofporcinemesenchymalstemcellsapplicationofnbhnme1asahumannme1suppressor-2021","author_short":["Cho, J. H.","Ju, W. S.","Seo, S. Y.","Kim, B. H.","Kim, J.","Kim, J.","Park, S. J.","Choo, Y."],"bibdata":{"bibtype":"article","type":"article","title":"The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells: Application of NB-hNME1 as a Human NME1 Suppressor","volume":"22","issn":"1422-0067","shorttitle":"The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells","url":"https://www.mdpi.com/1422-0067/22/22/12194","doi":"10.3390/ijms222212194","abstract":"This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.","language":"eng","number":"22","journal":"International Journal of Molecular Sciences","author":[{"propositions":[],"lastnames":["Cho"],"firstnames":["Jin","Hyoung"],"suffixes":[]},{"propositions":[],"lastnames":["Ju"],"firstnames":["Won","Seok"],"suffixes":[]},{"propositions":[],"lastnames":["Seo"],"firstnames":["Sang","Young"],"suffixes":[]},{"propositions":[],"lastnames":["Kim"],"firstnames":["Bo","Hyun"],"suffixes":[]},{"propositions":[],"lastnames":["Kim"],"firstnames":["Ji-Su"],"suffixes":[]},{"propositions":[],"lastnames":["Kim"],"firstnames":["Jong-Geol"],"suffixes":[]},{"propositions":[],"lastnames":["Park"],"firstnames":["Soon","Ju"],"suffixes":[]},{"propositions":[],"lastnames":["Choo"],"firstnames":["Young-Kug"],"suffixes":[]}],"month":"November","year":"2021","pmid":"34830075","keywords":"Application - Antibody Validation / Epitope Mapping, PEPperCHIP - Customized - Linear","pages":"12194","bibtex":"@article{cho_potential_2021,\n\ttitle = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}: {Application} of {NB}-{hNME1} as a {Human} {NME1} {Suppressor}},\n\tvolume = {22},\n\tissn = {1422-0067},\n\tshorttitle = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}},\n\turl = {https://www.mdpi.com/1422-0067/22/22/12194},\n\tdoi = {10.3390/ijms222212194},\n\tabstract = {This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. 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