The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells: Application of NB-hNME1 as a Human NME1 Suppressor. Cho, J. H., Ju, W. S., Seo, S. Y., Kim, B. H., Kim, J., Kim, J., Park, S. J., & Choo, Y. International Journal of Molecular Sciences, 22(22):12194, November, 2021.
The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells: Application of NB-hNME1 as a Human NME1 Suppressor [link]Paper  doi  abstract   bibtex   
This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.
@article{cho_potential_2021,
	title = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}: {Application} of {NB}-{hNME1} as a {Human} {NME1} {Suppressor}},
	volume = {22},
	issn = {1422-0067},
	shorttitle = {The {Potential} {Role} of {Human} {NME1} in {Neuronal} {Differentiation} of {Porcine} {Mesenchymal} {Stem} {Cells}},
	url = {https://www.mdpi.com/1422-0067/22/22/12194},
	doi = {10.3390/ijms222212194},
	abstract = {This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.},
	language = {eng},
	number = {22},
	journal = {International Journal of Molecular Sciences},
	author = {Cho, Jin Hyoung and Ju, Won Seok and Seo, Sang Young and Kim, Bo Hyun and Kim, Ji-Su and Kim, Jong-Geol and Park, Soon Ju and Choo, Young-Kug},
	month = nov,
	year = {2021},
	pmid = {34830075},
	keywords = {Application - Antibody Validation / Epitope Mapping, PEPperCHIP - Customized - Linear},
	pages = {12194},
}

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