Glucuronic acid in Arabidopsis thaliana xylans carries a novel pentose substituent. Chong, S. L., Koutaniemi, S., Juvonen, M., Derba-Maceluch, M., Mellerowicz, E. J., & Tenkanen, M. Int J Biol Macromol, 79:807–12, August, 2015. Edition: 2015/06/07
Paper doi abstract bibtex Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls.
@article{chong_glucuronic_2015,
title = {Glucuronic acid in {Arabidopsis} thaliana xylans carries a novel pentose substituent},
volume = {79},
issn = {1879-0003 (Electronic) 0141-8130 (Linking)},
url = {https://www.ncbi.nlm.nih.gov/pubmed/26047894},
doi = {10/f3pws3},
abstract = {Glucuronic acids in Arabidopsis thaliana xylans exist in 4-O-methylated (MeGlcA) and non-methylated (GlcA) forms at a ratio of about 3:2. The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. The results prove evidence of a new pentose substitution on the GlcA residue of Arabidopsis GX, which is likely present in the primary walls.},
language = {en},
urldate = {2021-06-07},
journal = {Int J Biol Macromol},
author = {Chong, S. L. and Koutaniemi, S. and Juvonen, M. and Derba-Maceluch, M. and Mellerowicz, E. J. and Tenkanen, M.},
month = aug,
year = {2015},
note = {Edition: 2015/06/07},
keywords = {Arabidopsis Proteins/chemistry/*genetics, Arabidopsis thaliana, Arabidopsis/*chemistry/genetics, Endo-1,4-beta Xylanases/genetics, Gene Expression Regulation, Plant, Glucuronic Acid/*chemistry/genetics, Glucuronoxylan, Oligosaccharides/chemistry, Pentoses/*chemistry/genetics, Pentosyltransferases/chemistry/*genetics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem mass spectrometry, Xylans/biosynthesis/chemistry/*genetics},
pages = {807--12},
}
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The matrix-assisted laser desorption/ionization mass spectrometry analysis of the endoxylanase liberated acidic oligosaccharides from the Arabidopsis inflorescence stem showed that two peaks with GlcA (GlcA-Xyl4Ac1 and GlcA-Xyl5Ac2) had abnormally high intensities, as well as different tandem mass spectra, than their 4-O-methylated counterparts. These peaks were interestingly enriched in the xylan biosynthesis mutant irx7 and irx9-1. Multi-stages fragmentation analysis using negative ion electrospray-ion trap mass spectrometry indicated that this GlcA was further carrying a pentose residue in the glucuronoxylan-derived oligosaccharide from irx9-1. The structure was also identified in Arabidopsis wild type. 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