Characterization of backcross generations obtained under field conditions from oilseed rape wild radish F-1 interspecific hybrids: an assessment of transgene dispersal. Chèvre, A., M., Eber, F., Baranger, A., Hureau, G., Barret, P., Picault, H., & Renard, M. Theoretical and Applied Genetics, 97(1-2):90-98, 1998. abstract bibtex Gene flow from glufosinate-resistant transgenic oilseed rape to wild radish was studied over two backcross generations. Under field conditions,seed production from oilseed rape-wild radish F-1 hybrids due to pollination by wild radish was always low: on average 0.12 and 0.78 seeds per 100 flowers and per plant, respectively. The cytogenetics of the resulting "BC1" plants can be explained in the main by three different genomic constitutions: either ACRrRr, 2n = 37, ACRr, 2n = 28 (the same chromosome number as the mother plant), or by the amphidiploid AACCRrRr, 2n = 56. The probability of gene exchange through chromosome pairing was high only in plants with Zn = 28 or 37 chromosomes. Due to the viability of unreduced or partially reduced female gametes, most of the "BC1" plants (81.9%) were Basta resistant whereas the analysis of oilseed rape specific loci indicated that their transmission varied with the locus. In spite of low male fertility (8.7%), an improvement of the female fertility over the F-1 hybrids was observed with an average production of 1.4 and 11 seeds per 100 flowers and per plant, respectively. At the following "BC2" generation, the bar gene transmission (57.2% of Basta-resistant plants) decreased as did the chromosome number, with a majority of plants having between 24 and 27 chromosomes, with 10.5% similar to wild radish (2n = 18). The lower the chromosome number, the better the fertility of the "BC2" plants. On average, 7.9 and 229.3 seeds per 100 flowers and per plant were produced. Gene-flow assessment is discussed based on these data.
@article{
title = {Characterization of backcross generations obtained under field conditions from oilseed rape wild radish F-1 interspecific hybrids: an assessment of transgene dispersal},
type = {article},
year = {1998},
pages = {90-98},
volume = {97},
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abstract = {Gene flow from glufosinate-resistant transgenic oilseed rape to wild radish was studied over two backcross generations. Under field conditions,seed production from oilseed rape-wild radish F-1 hybrids due to pollination by wild radish was always low: on average 0.12 and 0.78 seeds per 100 flowers and per plant, respectively. The cytogenetics of the resulting "BC1" plants can be explained in the main by three different genomic constitutions: either ACRrRr, 2n = 37, ACRr, 2n = 28 (the same chromosome number as the mother plant), or by the amphidiploid AACCRrRr, 2n = 56. The probability of gene exchange through chromosome pairing was high only in plants with Zn = 28 or 37 chromosomes. Due to the viability of unreduced or partially reduced female gametes, most of the "BC1" plants (81.9%) were Basta resistant whereas the analysis of oilseed rape specific loci indicated that their transmission varied with the locus. In spite of low male fertility (8.7%), an improvement of the female fertility over the F-1 hybrids was observed with an average production of 1.4 and 11 seeds per 100 flowers and per plant, respectively. At the following "BC2" generation, the bar gene transmission (57.2% of Basta-resistant plants) decreased as did the chromosome number, with a majority of plants having between 24 and 27 chromosomes, with 10.5% similar to wild radish (2n = 18). The lower the chromosome number, the better the fertility of the "BC2" plants. On average, 7.9 and 229.3 seeds per 100 flowers and per plant were produced. Gene-flow assessment is discussed based on these data.},
bibtype = {article},
author = {Chèvre, A M and Eber, F and Baranger, A and Hureau, G and Barret, P and Picault, H and Renard, M},
journal = {Theoretical and Applied Genetics},
number = {1-2}
}