@article{cian_fluorescence-based_2007,
	title = {Fluorescence-based melting assays for studying quadruplex ligands},
	volume = {42},
	issn = {1046-2023},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/17472900},
	doi = {10.1016/j.ymeth.2006.10.004},
	abstract = {The telomeric G-rich single-stranded DNA can adopt in vitro an intramolecular quadruplex structure, which has been shown to directly inhibit telomerase activity. The reactivation of this enzyme in immortalized and most cancer cells suggests that telomeres and telomerase are relevant targets in oncology, and telomere ligands and telomerase inhibitors have been proposed as new potential anticancer agents. In this paper, we have analysed the FRET method used to measure the stabilization and selectivity of quadruplex ligands towards the human telomeric G-quadruplex. The stabilization value depends on the nature of the fluorescent tags, the incubation buffer, and the method chosen for T-m calculation, complicating a direct comparison of the results obtained by different laboratories. (c) 2006 Elsevier Inc. All rights reserved.},
	number = {2},
	journal = {Methods-a Companion to Methods in Enzymology},
	author = {Cian, Anne De and Guittat, Lionel and Kaiser, Markus and Saccà, Barbara and Amrane, Samir and Bourdoncle, Anne and Alberti, Patrizia and Teulade-Fichou, Marie-paule P and Lacroix, Laurent and Mergny, Jean-Louis L and Sacca, B and Cian, Anne De},
	month = jul,
	year = {2007},
	pmid = {17472900},
	note = {tex.ids= DECIAN2007, cianFluorescencebasedMeltingAssays2007a, decianFluorescencebasedMeltingAssays2007, decianFluorescencebasedMeltingAssays2007a
tex.city: Museum Natl Hist Nat, Biophys Lab, INSERM, USM 503,CNRS,UMR 5153, F-75231 Paris 05, France. Coll France, CNRS, UPR 285, Lab Chim Interact Mol, F-75005 Paris, France. Mergny, JL, Museum Natl Hist Nat, Biophys Lab, INSERM, USM 503,CNRS,UMR 5153, 43 Rue Cuvi
ISBN: 1046-2023},
	keywords = {\#nosource, Base Sequence, Buffers, Chemical, DNA, DNA ligands, Enzyme Inhibitors, Enzyme Inhibitors: chemistry, FRET, FRET telomeres telomerase inhibitor G-quadruplex G, Fluorescence, Fluorescence Resonance Energy Transfer, G-Quadruplexes, G-quadruplex, G-quartet, Guanine, Guanine: chemistry, Hot Temperature, Humans, Ligands, Models, Nucleic Acid Conformation, Single-Stranded, Single-Stranded: chemistry, Telomerase, Telomerase inhibitor, Telomerase: antagonists \& inhibitors, Telomerase: chemistry, Telomerase: genetics, Telomere, Telomere: chemistry, Telomeres, dna ligands, fret, g-quadruplex, g-quartet, telomerase inhibitor, telomeres},
	pages = {183--195 ST -- Fluorescence--based melting assays fo},
}

{"_id":"4ok66s2LagFqyoMgg","bibbaseid":"cian-guittat-kaiser-sacc-amrane-bourdoncle-alberti-teuladefichou-etal-fluorescencebasedmeltingassaysforstudyingquadruplexligands-2007","author_short":["Cian, A. D.","Guittat, L.","Kaiser, M.","Saccà, B.","Amrane, S.","Bourdoncle, A.","Alberti, P.","Teulade-Fichou, M. P","Lacroix, L.","Mergny, J. L","Sacca, B","Cian, A. D."],"bibdata":{"bibtype":"article","type":"article","title":"Fluorescence-based melting assays for studying quadruplex ligands","volume":"42","issn":"1046-2023","url":"http://www.ncbi.nlm.nih.gov/pubmed/17472900","doi":"10.1016/j.ymeth.2006.10.004","abstract":"The telomeric G-rich single-stranded DNA can adopt in vitro an intramolecular quadruplex structure, which has been shown to directly inhibit telomerase activity. The reactivation of this enzyme in immortalized and most cancer cells suggests that telomeres and telomerase are relevant targets in oncology, and telomere ligands and telomerase inhibitors have been proposed as new potential anticancer agents. In this paper, we have analysed the FRET method used to measure the stabilization and selectivity of quadruplex ligands towards the human telomeric G-quadruplex. The stabilization value depends on the nature of the fluorescent tags, the incubation buffer, and the method chosen for T-m calculation, complicating a direct comparison of the results obtained by different laboratories. (c) 2006 Elsevier Inc. All rights reserved.","number":"2","journal":"Methods-a Companion to Methods in Enzymology","author":[{"propositions":[],"lastnames":["Cian"],"firstnames":["Anne","De"],"suffixes":[]},{"propositions":[],"lastnames":["Guittat"],"firstnames":["Lionel"],"suffixes":[]},{"propositions":[],"lastnames":["Kaiser"],"firstnames":["Markus"],"suffixes":[]},{"propositions":[],"lastnames":["Saccà"],"firstnames":["Barbara"],"suffixes":[]},{"propositions":[],"lastnames":["Amrane"],"firstnames":["Samir"],"suffixes":[]},{"propositions":[],"lastnames":["Bourdoncle"],"firstnames":["Anne"],"suffixes":[]},{"propositions":[],"lastnames":["Alberti"],"firstnames":["Patrizia"],"suffixes":[]},{"propositions":[],"lastnames":["Teulade-Fichou"],"firstnames":["Marie-paule","P"],"suffixes":[]},{"propositions":[],"lastnames":["Lacroix"],"firstnames":["Laurent"],"suffixes":[]},{"propositions":[],"lastnames":["Mergny"],"firstnames":["Jean-Louis","L"],"suffixes":[]},{"propositions":[],"lastnames":["Sacca"],"firstnames":["B"],"suffixes":[]},{"propositions":[],"lastnames":["Cian"],"firstnames":["Anne","De"],"suffixes":[]}],"month":"July","year":"2007","pmid":"17472900","note":"tex.ids= DECIAN2007, cianFluorescencebasedMeltingAssays2007a, decianFluorescencebasedMeltingAssays2007, decianFluorescencebasedMeltingAssays2007a tex.city: Museum Natl Hist Nat, Biophys Lab, INSERM, USM 503,CNRS,UMR 5153, F-75231 Paris 05, France. Coll France, CNRS, UPR 285, Lab Chim Interact Mol, F-75005 Paris, France. Mergny, JL, Museum Natl Hist Nat, Biophys Lab, INSERM, USM 503,CNRS,UMR 5153, 43 Rue Cuvi ISBN: 1046-2023","keywords":"#nosource, Base Sequence, Buffers, Chemical, DNA, DNA ligands, Enzyme Inhibitors, Enzyme Inhibitors: chemistry, FRET, FRET telomeres telomerase inhibitor G-quadruplex G, Fluorescence, Fluorescence Resonance Energy Transfer, G-Quadruplexes, G-quadruplex, G-quartet, Guanine, Guanine: chemistry, Hot Temperature, Humans, Ligands, Models, Nucleic Acid Conformation, Single-Stranded, Single-Stranded: chemistry, Telomerase, Telomerase inhibitor, Telomerase: antagonists & inhibitors, Telomerase: chemistry, Telomerase: genetics, Telomere, Telomere: chemistry, Telomeres, dna ligands, fret, g-quadruplex, g-quartet, telomerase inhibitor, telomeres","pages":"183–195 ST – Fluorescence–based melting assays fo","bibtex":"@article{cian_fluorescence-based_2007,\n\ttitle = {Fluorescence-based melting assays for studying quadruplex ligands},\n\tvolume = {42},\n\tissn = {1046-2023},\n\turl = {http://www.ncbi.nlm.nih.gov/pubmed/17472900},\n\tdoi = {10.1016/j.ymeth.2006.10.004},\n\tabstract = {The telomeric G-rich single-stranded DNA can adopt in vitro an intramolecular quadruplex structure, which has been shown to directly inhibit telomerase activity. The reactivation of this enzyme in immortalized and most cancer cells suggests that telomeres and telomerase are relevant targets in oncology, and telomere ligands and telomerase inhibitors have been proposed as new potential anticancer agents. In this paper, we have analysed the FRET method used to measure the stabilization and selectivity of quadruplex ligands towards the human telomeric G-quadruplex. The stabilization value depends on the nature of the fluorescent tags, the incubation buffer, and the method chosen for T-m calculation, complicating a direct comparison of the results obtained by different laboratories. (c) 2006 Elsevier Inc. All rights reserved.},\n\tnumber = {2},\n\tjournal = {Methods-a Companion to Methods in Enzymology},\n\tauthor = {Cian, Anne De and Guittat, Lionel and Kaiser, Markus and Saccà, Barbara and Amrane, Samir and Bourdoncle, Anne and Alberti, Patrizia and Teulade-Fichou, Marie-paule P and Lacroix, Laurent and Mergny, Jean-Louis L and Sacca, B and Cian, Anne De},\n\tmonth = jul,\n\tyear = {2007},\n\tpmid = {17472900},\n\tnote = {tex.ids= DECIAN2007, cianFluorescencebasedMeltingAssays2007a, decianFluorescencebasedMeltingAssays2007, decianFluorescencebasedMeltingAssays2007a\ntex.city: Museum Natl Hist Nat, Biophys Lab, INSERM, USM 503,CNRS,UMR 5153, F-75231 Paris 05, France. Coll France, CNRS, UPR 285, Lab Chim Interact Mol, F-75005 Paris, France. 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