@article{RN194,
   author = {Colacino, K. R. and Arena, C. B. and Dong, S. and Roman, M. and Davalos, R. V. and Lee, Y. W.},
   title = {Folate Conjugated Cellulose Nanocrystals Potentiate Irreversible Electroporation-induced Cytotoxicity for the Selective Treatment of Cancer Cells},
   journal = {Technol Cancer Res Treat},
   volume = {14},
   number = {6},
   pages = {757-66},
   note = {1533-0338
Colacino, Katelyn R
Arena, Christopher B
Dong, Shuping
Roman, Maren
Davalos, Rafael V
Lee, Yong W
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
United States
2014/04/23
Technol Cancer Res Treat. 2015 Dec;14(6):757-66. doi: 10.7785/tcrt.2012.500428. Epub 2014 Nov 26.},
   abstract = {Cellulose nanocrystals are rod-shaped, crystalline nanoparticles that have shown prom-ise in a number of industrial applications for their unique chemical and physical properties. However, investigations of their abilities in the biomedical field are limited. The goal of this study is to show the potential use of folic acid-conjugated cellulose nanocrystals in the potentiation of irreversible electroporation-induced cell death in folate receptor (FR)-positive cancers. We optimized key pulse parameters including pulse duration, intensity, and incubation time with nanoparticles prior to electroporation. FR-positive cancer cells, KB and MDA-MB-468, were preincubated with cellulose nanocrystals (CNCs) conjugated with the targeting molecule folic acid (FA), 10 and 20 min respectively, prior to application of the optimized pulse electric field (PEF), 600 and 500 V/cm respectively. We have shown cellulose nanocrystals' ability to potentiate a new technique for tumor ablation, irreversible electroporation. Pre-incubation with FA-conjugated CNCs (CNC-FA) has shown a significant increase in cytotoxicity induced by irreversible electroporation in FR-positive cancer cells, KB and MDA-MB-468. Non-targeted CNCs (CNC-COOH) did not potentiate IRE when preincubated at the same parameters as previously stated in these cell types. In addition, CNC-FA did not potentiate irreversible electroporation-induced cytotoxicity in a FR-negative cancer cell type, A549. Without changing irreversible electroporation parameters it is possible to increase the cytotoxic effect on FR-positive cancer cells by exploiting the specific binding of FA to the FR, while not causing further damage to FR-negative tissue.},
   keywords = {Cell Line, Tumor
Cellulose
Drug Delivery Systems/*methods
Electrochemotherapy/*methods
Finite Element Analysis
Folic Acid/*administration & dosage
Humans
Microscopy, Confocal
*Nanoparticles
Cancer treatment
Folate receptor
Targeted drug delivery},
   ISSN = {1533-0338},
   DOI = {10.7785/tcrt.2012.500428},
   year = {2015},
   type = {Journal Article}
}

{"_id":"ET7g5fCphMoboN5Su","bibbaseid":"colacino-arena-dong-roman-davalos-lee-folateconjugatedcellulosenanocrystalspotentiateirreversibleelectroporationinducedcytotoxicityfortheselectivetreatmentofcancercells-2015","author_short":["Colacino, K. R.","Arena, C. B.","Dong, S.","Roman, M.","Davalos, R. V.","Lee, Y. W."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Colacino"],"firstnames":["K.","R."],"suffixes":[]},{"propositions":[],"lastnames":["Arena"],"firstnames":["C.","B."],"suffixes":[]},{"propositions":[],"lastnames":["Dong"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Roman"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Lee"],"firstnames":["Y.","W."],"suffixes":[]}],"title":"Folate Conjugated Cellulose Nanocrystals Potentiate Irreversible Electroporation-induced Cytotoxicity for the Selective Treatment of Cancer Cells","journal":"Technol Cancer Res Treat","volume":"14","number":"6","pages":"757-66","note":"1533-0338 Colacino, Katelyn R Arena, Christopher B Dong, Shuping Roman, Maren Davalos, Rafael V Lee, Yong W Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. United States 2014/04/23 Technol Cancer Res Treat. 2015 Dec;14(6):757-66. doi: 10.7785/tcrt.2012.500428. Epub 2014 Nov 26.","abstract":"Cellulose nanocrystals are rod-shaped, crystalline nanoparticles that have shown prom-ise in a number of industrial applications for their unique chemical and physical properties. However, investigations of their abilities in the biomedical field are limited. The goal of this study is to show the potential use of folic acid-conjugated cellulose nanocrystals in the potentiation of irreversible electroporation-induced cell death in folate receptor (FR)-positive cancers. We optimized key pulse parameters including pulse duration, intensity, and incubation time with nanoparticles prior to electroporation. FR-positive cancer cells, KB and MDA-MB-468, were preincubated with cellulose nanocrystals (CNCs) conjugated with the targeting molecule folic acid (FA), 10 and 20 min respectively, prior to application of the optimized pulse electric field (PEF), 600 and 500 V/cm respectively. We have shown cellulose nanocrystals' ability to potentiate a new technique for tumor ablation, irreversible electroporation. Pre-incubation with FA-conjugated CNCs (CNC-FA) has shown a significant increase in cytotoxicity induced by irreversible electroporation in FR-positive cancer cells, KB and MDA-MB-468. Non-targeted CNCs (CNC-COOH) did not potentiate IRE when preincubated at the same parameters as previously stated in these cell types. In addition, CNC-FA did not potentiate irreversible electroporation-induced cytotoxicity in a FR-negative cancer cell type, A549. Without changing irreversible electroporation parameters it is possible to increase the cytotoxic effect on FR-positive cancer cells by exploiting the specific binding of FA to the FR, while not causing further damage to FR-negative tissue.","keywords":"Cell Line, Tumor Cellulose Drug Delivery Systems/*methods Electrochemotherapy/*methods Finite Element Analysis Folic Acid/*administration & dosage Humans Microscopy, Confocal *Nanoparticles Cancer treatment Folate receptor Targeted drug delivery","issn":"1533-0338","doi":"10.7785/tcrt.2012.500428","year":"2015","bibtex":"@article{RN194,\n author = {Colacino, K. R. and Arena, C. B. and Dong, S. and Roman, M. and Davalos, R. V. and Lee, Y. W.},\n title = {Folate Conjugated Cellulose Nanocrystals Potentiate Irreversible Electroporation-induced Cytotoxicity for the Selective Treatment of Cancer Cells},\n journal = {Technol Cancer Res Treat},\n volume = {14},\n number = {6},\n pages = {757-66},\n note = {1533-0338\nColacino, Katelyn R\nArena, Christopher B\nDong, Shuping\nRoman, Maren\nDavalos, Rafael V\nLee, Yong W\nJournal Article\nResearch Support, Non-U.S. Gov't\nResearch Support, U.S. Gov't, Non-P.H.S.\nUnited States\n2014/04/23\nTechnol Cancer Res Treat. 2015 Dec;14(6):757-66. doi: 10.7785/tcrt.2012.500428. Epub 2014 Nov 26.},\n abstract = {Cellulose nanocrystals are rod-shaped, crystalline nanoparticles that have shown prom-ise in a number of industrial applications for their unique chemical and physical properties. However, investigations of their abilities in the biomedical field are limited. The goal of this study is to show the potential use of folic acid-conjugated cellulose nanocrystals in the potentiation of irreversible electroporation-induced cell death in folate receptor (FR)-positive cancers. We optimized key pulse parameters including pulse duration, intensity, and incubation time with nanoparticles prior to electroporation. FR-positive cancer cells, KB and MDA-MB-468, were preincubated with cellulose nanocrystals (CNCs) conjugated with the targeting molecule folic acid (FA), 10 and 20 min respectively, prior to application of the optimized pulse electric field (PEF), 600 and 500 V/cm respectively. We have shown cellulose nanocrystals' ability to potentiate a new technique for tumor ablation, irreversible electroporation. Pre-incubation with FA-conjugated CNCs (CNC-FA) has shown a significant increase in cytotoxicity induced by irreversible electroporation in FR-positive cancer cells, KB and MDA-MB-468. Non-targeted CNCs (CNC-COOH) did not potentiate IRE when preincubated at the same parameters as previously stated in these cell types. In addition, CNC-FA did not potentiate irreversible electroporation-induced cytotoxicity in a FR-negative cancer cell type, A549. Without changing irreversible electroporation parameters it is possible to increase the cytotoxic effect on FR-positive cancer cells by exploiting the specific binding of FA to the FR, while not causing further damage to FR-negative tissue.},\n keywords = {Cell Line, Tumor\nCellulose\nDrug Delivery Systems/*methods\nElectrochemotherapy/*methods\nFinite Element Analysis\nFolic Acid/*administration & dosage\nHumans\nMicroscopy, Confocal\n*Nanoparticles\nCancer treatment\nFolate receptor\nTargeted drug delivery},\n ISSN = {1533-0338},\n DOI = {10.7785/tcrt.2012.500428},\n year = {2015},\n type = {Journal Article}\n}\n\n","author_short":["Colacino, K. R.","Arena, C. B.","Dong, S.","Roman, M.","Davalos, R. V.","Lee, Y. W."],"key":"RN194","id":"RN194","bibbaseid":"colacino-arena-dong-roman-davalos-lee-folateconjugatedcellulosenanocrystalspotentiateirreversibleelectroporationinducedcytotoxicityfortheselectivetreatmentofcancercells-2015","role":"author","urls":{},"keyword":["Cell Line","Tumor Cellulose Drug Delivery Systems/*methods Electrochemotherapy/*methods Finite Element Analysis Folic Acid/*administration & dosage Humans Microscopy","Confocal *Nanoparticles Cancer treatment Folate receptor Targeted drug delivery"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["cell line","tumor cellulose drug delivery systems/*methods electrochemotherapy/*methods finite element analysis folic acid/*administration & dosage humans microscopy","confocal *nanoparticles cancer treatment folate receptor targeted drug delivery"],"search_terms":["folate","conjugated","cellulose","nanocrystals","potentiate","irreversible","electroporation","induced","cytotoxicity","selective","treatment","cancer","cells","colacino","arena","dong","roman","davalos","lee"],"title":"Folate Conjugated Cellulose Nanocrystals Potentiate Irreversible Electroporation-induced Cytotoxicity for the Selective Treatment of Cancer Cells","year":2015}