Inducible gene expression systems and plant biotechnology. Corrado, G. & Karali, M. Biotechnology Advances.
abstract   bibtex   
Plant biotechnology relies heavily on the genetic manipulation of crops. Almost invariantly, the gene of interest is expressed in a constitutive fashion, although this may not be strictly necessary for several applications. Currently, there are several regulatable expression systems for the temporal, spatial and quantitative control of transgene activity. These molecular switches are based on components derived from different organisms, which range from viruses to higher eukaryotes. Many inducible systems have been designed for fundamental and applied research and since their initial development, they have become increasingly popular in plant molecular biology. This review covers a broad number of inducible expression systems examining their properties and relevance for plant biotechnology in its various guises, from molecular breeding to pharmaceutical and industrial applications. For each system, we examine some advantages and limitations, also in relation to the strategy on which they rely. Besides being necessary to control useful genes that may negatively affect crop yield and quality, we discuss that inducible systems can be also used to increase public acceptance of GMOs, reducing some of the most common concerns. Finally, we suggest some directions and future developments for their further diffusion in agriculture and biotechnology.
@article{
 title = {Inducible gene expression systems and plant biotechnology},
 type = {article},
 identifiers = {[object Object]},
 keywords = {Chemical inducer,GMO,Genetic engineering,Gurts,Marker-free,Promoter,Recombinant proteins,Regulated gene expression,Transgenic plants},
 volume = {In Press, },
 websites = {http://www.sciencedirect.com/science/article/B6T4X-4WB3NB9-2/2/9a92f54f61523d144f4e51555d47f168},
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 last_modified = {2012-01-05T12:54:29.000Z},
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 abstract = {Plant biotechnology relies heavily on the genetic manipulation of crops. Almost invariantly, the gene of interest is expressed in a constitutive fashion, although this may not be strictly necessary for several applications. Currently, there are several regulatable expression systems for the temporal, spatial and quantitative control of transgene activity. These molecular switches are based on components derived from different organisms, which range from viruses to higher eukaryotes. Many inducible systems have been designed for fundamental and applied research and since their initial development, they have become increasingly popular in plant molecular biology. This review covers a broad number of inducible expression systems examining their properties and relevance for plant biotechnology in its various guises, from molecular breeding to pharmaceutical and industrial applications. For each system, we examine some advantages and limitations, also in relation to the strategy on which they rely. Besides being necessary to control useful genes that may negatively affect crop yield and quality, we discuss that inducible systems can be also used to increase public acceptance of GMOs, reducing some of the most common concerns. Finally, we suggest some directions and future developments for their further diffusion in agriculture and biotechnology.},
 bibtype = {article},
 author = {Corrado, Giandomenico and Karali, Marianthi},
 journal = {Biotechnology Advances}
}

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