Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Cossarizza, A., Chang, H., Radbruch, A., Acs, A., Adam, D., Adam-Klages, S., Agace, W. W, Aghaeepour, N., Akdis, M., Allez, M., Almeida, L. N., Alvisi, G., Anderson, G., Andrä, I., Annunziato, F., Anselmo, A., Bacher, P., Baldari, C. T, Bari, S., Barnaba, V., Barros-Martins, J., Battistini, L., Bauer, W., Baumgart, S., Baumgarth, N., Baumjohann, D., Baying, B., Bebawy, M., Becher, B., Beisker, W., Benes, V., Beyaert, R., Blanco, A., Boardman, D. A, Bogdan, C., Borger, J. G, Borsellino, G., Boulais, P. E, Bradford, J. A, Brenner, D., Brinkman, R. R, Brooks, A. E S, Busch, D. H, Büscher, M., Bushnell, T. P, Calzetti, F., Cameron, G., Cammarata, I., Cao, X., Cardell, S. L, Casola, S., Cassatella, M. A, Cavani, A., Celada, A., Chatenoud, L., Chattopadhyay, P. K, Chow, S., Christakou, E., Čičin-Šain, L., Clerici, M., Colombo, F. S, Cook, L., Cooke, A., Cooper, A. M, Corbett, A. J, Cosma, A., Cosmi, L., Coulie, P. G, Cumano, A., Cvetkovic, L., Dang, V. D., Dang-Heine, C., Davey, M. S, Davies, D., De Biasi, S., Del Zotto, G., Dela Cruz, G. V., Delacher, M., Della Bella, S., Dellabona, P., Deniz, G., Dessing, M., Di Santo, J. P, Diefenbach, A., Dieli, F., Dolf, A., Dörner, T., Dress, R. J, Dudziak, D., Dustin, M., Dutertre, C., Ebner, F., Eckle, S. B G, Edinger, M., Eede, P., Ehrhardt, G. R A, Eich, M., Engel, P., Engelhardt, B., Erdei, A., Esser, C., Everts, B., Evrard, M., Falk, C. S, Fehniger, T. A, Felipo-Benavent, M., Ferry, H., Feuerer, M., Filby, A., Filkor, K., Fillatreau, S., Follo, M., Förster, I., Foster, J., Foulds, G. A, Frehse, B., Frenette, P. S, Frischbutter, S., Fritzsche, W., Galbraith, D. W, Gangaev, A., Garbi, N., Gaudilliere, B., Gazzinelli, R. T, Geginat, J., Gerner, W., Gherardin, N. A, Ghoreschi, K., Gibellini, L., Ginhoux, F., Goda, K., Godfrey, D. I, Goettlinger, C., González-Navajas, J. M, Goodyear, C. S, Gori, A., Grogan, J. L, Grummitt, D., Grützkau, A., Haftmann, C., Hahn, J., Hammad, H., Hämmerling, G., Hansmann, L., Hansson, G., Harpur, C. M, Hartmann, S., Hauser, A., Hauser, A. E, Haviland, D. L, Hedley, D., Hernández, D. C, Herrera, G., Herrmann, M., Hess, C., Höfer, T., Hoffmann, P., Hogquist, K., Holland, T., Höllt, T., Holmdahl, R., Hombrink, P., Houston, J. P, Hoyer, B. F, Huang, B., Huang, F., Huber, J. E, Huehn, J., Hundemer, M., Hunter, C. A, Hwang, W. Y K, Iannone, A., Ingelfinger, F., Ivison, S. M, Jäck, H., Jani, P. K, Jávega, B., Jonjic, S., Kaiser, T., Kalina, T., Kamradt, T., Kaufmann, S. H E, Keller, B., Ketelaars, S. L C, Khalilnezhad, A., Khan, S., Kisielow, J., Klenerman, P., Knopf, J., Koay, H., Kobow, K., Kolls, J. K, Kong, W. T., Kopf, M., Korn, T., Kriegsmann, K., Kristyanto, H., Kroneis, T., Krueger, A., Kühne, J., Kukat, C., Kunkel, D., Kunze-Schumacher, H., Kurosaki, T., Kurts, C., Kvistborg, P., Kwok, I., Landry, J., Lantz, O., Lanuti, P., LaRosa, F., Lehuen, A., LeibundGut-Landmann, S., Leipold, M. D, Leung, L. Y T, Levings, M. K, Lino, A. C, Liotta, F., Litwin, V., Liu, Y., Ljunggren, H., Lohoff, M., Lombardi, G., Lopez, L., López-Botet, M., Lovett-Racke, A. E, Lubberts, E., Luche, H., Ludewig, B., Lugli, E., Lunemann, S., Maecker, H. T, Maggi, L., Maguire, O., Mair, F., Mair, K. H, Mantovani, A., Manz, R. A, Marshall, A. J, Martínez-Romero, A., Martrus, G., Marventano, I., Maslinski, W., Matarese, G., Mattioli, A. V., Maueröder, C., Mazzoni, A., McCluskey, J., McGrath, M., McGuire, H. M, McInnes, I. B, Mei, H. E, Melchers, F., Melzer, S., Mielenz, D., Miller, S. D, Mills, K. H G, Minderman, H., Mjösberg, J., Moore, J., Moran, B., Moretta, L., Mosmann, T. R, Müller, S., Multhoff, G., Muñoz, L. E., Münz, C., Nakayama, T., Nasi, M., Neumann, K., Ng, L. G., Niedobitek, A., Nourshargh, S., Núñez, G., O'Connor, J., Ochel, A., Oja, A., Ordonez, D., Orfao, A., Orlowski-Oliver, E., Ouyang, W., Oxenius, A., Palankar, R., Panse, I., Pattanapanyasat, K., Paulsen, M., Pavlinic, D., Penter, L., Peterson, P., Peth, C., Petriz, J., Piancone, F., Pickl, W. F, Piconese, S., Pinti, M., Pockley, A G., Podolska, M. J., Poon, Z., Pracht, K., Prinz, I., Pucillo, C. E M, Quataert, S. A, Quatrini, L., Quinn, K. M, Radbruch, H., Radstake, T. R D J, Rahmig, S., Rahn, H., Rajwa, B., Ravichandran, G., Raz, Y., Rebhahn, J. A, Recktenwald, D., Reimer, D., e Sousa, C., Remmerswaal, E. B M, Richter, L., Rico, L. G, Riddell, A., Rieger, A. M, Robinson, J P., Romagnani, C., Rubartelli, A., Ruland, J., Saalmüller, A., Saeys, Y., Saito, T., Sakaguchi, S., Sala-de-Oyanguren, F., Samstag, Y., Sanderson, S., Sandrock, I., Santoni, A., Sanz, R. B., Saresella, M., Sautes-Fridman, C., Sawitzki, B., Schadt, L., Scheffold, A., Scherer, H. U, Schiemann, M., Schildberg, F. A, Schimisky, E., Schlitzer, A., Schlosser, J., Schmid, S., Schmitt, S., Schober, K., Schraivogel, D., Schuh, W., Schüler, T., Schulte, R., Schulz, A. R., Schulz, S. R, Scottá, C., Scott-Algara, D., Sester, D. P, Shankey, T V., Silva-Santos, B., Simon, A. K., Sitnik, K. M, Sozzani, S., Speiser, D. E, Spidlen, J., Stahlberg, A., Stall, A. M, Stanley, N., Stark, R., Stehle, C., Steinmetz, T., Stockinger, H., Takahama, Y., Takeda, K., Tan, L., Tárnok, A., Tiegs, G., Toldi, G., Tornack, J., Traggiai, E., Trebak, M., Tree, T. I M, Trotter, J., Trowsdale, J., Tsoumakidou, M., Ulrich, H., Urbanczyk, S., van de Veen, W., van den Broek, M., van der Pol, E., Van Gassen, S., Van Isterdael, G., van Lier, R. A W, Veldhoen, M., Vento-Asturias, S., Vieira, P., Voehringer, D., Volk, H., von Borstel, A., von Volkmann, K., Waisman, A., Walker, R. V, Wallace, P. K, Wang, S. A, Wang, X. M, Ward, M. D, Ward-Hartstonge, K. A, Warnatz, K., Warnes, G., Warth, S., Waskow, C., Watson, J. V, Watzl, C., Wegener, L., Weisenburger, T., Wiedemann, A., Wienands, J., Wilharm, A., Wilkinson, R. J, Willimsky, G., Wing, J. B, Winkelmann, R., Winkler, T. H, Wirz, O. F, Wong, A., Wurst, P., Yang, J. H M, Yang, J., Yazdanbakhsh, M., Yu, L., Yue, A., Zhang, H., Zhao, Y., Ziegler, S. M., Zielinski, C., Zimmermann, J., & Zychlinsky, A. European Journal of Immunology, 49(10):1457–1973, 2019.
Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition) [link]Paper  doi  abstract   bibtex   
Abstract These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
@article{doi:10.1002/eji.201970107,
abstract = {Abstract These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.},
author = {Cossarizza, Andrea and Chang, Hyun-Dong and Radbruch, Andreas and Acs, Andreas and Adam, Dieter and Adam-Klages, Sabine and Agace, William W and Aghaeepour, Nima and Akdis, M{\"{u}}beccel and Allez, Matthieu and Almeida, Larissa Nogueira and Alvisi, Giorgia and Anderson, Graham and Andr{\"{a}}, Immanuel and Annunziato, Francesco and Anselmo, Achille and Bacher, Petra and Baldari, Cosima T and Bari, Sudipto and Barnaba, Vincenzo and Barros-Martins, Joana and Battistini, Luca and Bauer, Wolfgang and Baumgart, Sabine and Baumgarth, Nicole and Baumjohann, Dirk and Baying, Bianka and Bebawy, Mary and Becher, Burkhard and Beisker, Wolfgang and Benes, Vladimir and Beyaert, Rudi and Blanco, Alfonso and Boardman, Dominic A and Bogdan, Christian and Borger, Jessica G and Borsellino, Giovanna and Boulais, Philip E and Bradford, Jolene A and Brenner, Dirk and Brinkman, Ryan R and Brooks, Anna E S and Busch, Dirk H and B{\"{u}}scher, Martin and Bushnell, Timothy P and Calzetti, Federica and Cameron, Garth and Cammarata, Ilenia and Cao, Xuetao and Cardell, Susanna L and Casola, Stefano and Cassatella, Marco A and Cavani, Andrea and Celada, Antonio and Chatenoud, Lucienne and Chattopadhyay, Pratip K and Chow, Sue and Christakou, Eleni and {\v{C}}i{\v{c}}in-{\v{S}}ain, Luka and Clerici, Mario and Colombo, Federico S and Cook, Laura and Cooke, Anne and Cooper, Andrea M and Corbett, Alexandra J and Cosma, Antonio and Cosmi, Lorenzo and Coulie, Pierre G and Cumano, Ana and Cvetkovic, Ljiljana and Dang, Van Duc and Dang-Heine, Chantip and Davey, Martin S and Davies, Derek and {De Biasi}, Sara and {Del Zotto}, Genny and {Dela Cruz}, Gelo Victoriano and Delacher, Michael and {Della Bella}, Silvia and Dellabona, Paolo and Deniz, G{\"{u}}nnur and Dessing, Mark and {Di Santo}, James P and Diefenbach, Andreas and Dieli, Francesco and Dolf, Andreas and D{\"{o}}rner, Thomas and Dress, Regine J and Dudziak, Diana and Dustin, Michael and Dutertre, Charles-Antoine and Ebner, Friederike and Eckle, Sidonia B G and Edinger, Matthias and Eede, Pascale and Ehrhardt, G{\"{o}}tz R A and Eich, Marcus and Engel, Pablo and Engelhardt, Britta and Erdei, Anna and Esser, Charlotte and Everts, Bart and Evrard, Maximilien and Falk, Christine S and Fehniger, Todd A and Felipo-Benavent, Mar and Ferry, Helen and Feuerer, Markus and Filby, Andrew and Filkor, Kata and Fillatreau, Simon and Follo, Marie and F{\"{o}}rster, Irmgard and Foster, John and Foulds, Gemma A and Frehse, Britta and Frenette, Paul S and Frischbutter, Stefan and Fritzsche, Wolfgang and Galbraith, David W and Gangaev, Anastasia and Garbi, Natalio and Gaudilliere, Brice and Gazzinelli, Ricardo T and Geginat, Jens and Gerner, Wilhelm and Gherardin, Nicholas A and Ghoreschi, Kamran and Gibellini, Lara and Ginhoux, Florent and Goda, Keisuke and Godfrey, Dale I and Goettlinger, Christoph and Gonz{\'{a}}lez-Navajas, Jose M and Goodyear, Carl S and Gori, Andrea and Grogan, Jane L and Grummitt, Daryl and Gr{\"{u}}tzkau, Andreas and Haftmann, Claudia and Hahn, Jonas and Hammad, Hamida and H{\"{a}}mmerling, G{\"{u}}nter and Hansmann, Leo and Hansson, Goran and Harpur, Christopher M and Hartmann, Susanne and Hauser, Andrea and Hauser, Anja E and Haviland, David L and Hedley, David and Hern{\'{a}}ndez, Daniela C and Herrera, Guadalupe and Herrmann, Martin and Hess, Christoph and H{\"{o}}fer, Thomas and Hoffmann, Petra and Hogquist, Kristin and Holland, Tristan and H{\"{o}}llt, Thomas and Holmdahl, Rikard and Hombrink, Pleun and Houston, Jessica P and Hoyer, Bimba F and Huang, Bo and Huang, Fang-Ping and Huber, Johanna E and Huehn, Jochen and Hundemer, Michael and Hunter, Christopher A and Hwang, William Y K and Iannone, Anna and Ingelfinger, Florian and Ivison, Sabine M and J{\"{a}}ck, Hans-Martin and Jani, Peter K and J{\'{a}}vega, Beatriz and Jonjic, Stipan and Kaiser, Toralf and Kalina, Tomas and Kamradt, Thomas and Kaufmann, Stefan H E and Keller, Baerbel and Ketelaars, Steven L C and Khalilnezhad, Ahad and Khan, Srijit and Kisielow, Jan and Klenerman, Paul and Knopf, Jasmin and Koay, Hui-Fern and Kobow, Katja and Kolls, Jay K and Kong, Wan Ting and Kopf, Manfred and Korn, Thomas and Kriegsmann, Katharina and Kristyanto, Hendy and Kroneis, Thomas and Krueger, Andreas and K{\"{u}}hne, Jenny and Kukat, Christian and Kunkel, D{\'{e}}sir{\'{e}}e and Kunze-Schumacher, Heike and Kurosaki, Tomohiro and Kurts, Christian and Kvistborg, Pia and Kwok, Immanuel and Landry, Jonathan and Lantz, Olivier and Lanuti, Paola and LaRosa, Francesca and Lehuen, Agn{\`{e}}s and LeibundGut-Landmann, Salom{\'{e}} and Leipold, Michael D and Leung, Leslie Y T and Levings, Megan K and Lino, Andreia C and Liotta, Francesco and Litwin, Virginia and Liu, Yanling and Ljunggren, Hans-Gustaf and Lohoff, Michael and Lombardi, Giovanna and Lopez, Lilly and L{\'{o}}pez-Botet, Miguel and Lovett-Racke, Amy E and Lubberts, Erik and Luche, Herve and Ludewig, Burkhard and Lugli, Enrico and Lunemann, Sebastian and Maecker, Holden T and Maggi, Laura and Maguire, Orla and Mair, Florian and Mair, Kerstin H and Mantovani, Alberto and Manz, Rudolf A and Marshall, Aaron J and Mart{\'{i}}nez-Romero, Alicia and Martrus, Gl{\`{o}}ria and Marventano, Ivana and Maslinski, Wlodzimierz and Matarese, Giuseppe and Mattioli, Anna Vittoria and Mauer{\"{o}}der, Christian and Mazzoni, Alessio and McCluskey, James and McGrath, Mairi and McGuire, Helen M and McInnes, Iain B and Mei, Henrik E and Melchers, Fritz and Melzer, Susanne and Mielenz, Dirk and Miller, Stephen D and Mills, Kingston H G and Minderman, Hans and Mj{\"{o}}sberg, Jenny and Moore, Jonni and Moran, Barry and Moretta, Lorenzo and Mosmann, Tim R and M{\"{u}}ller, Susann and Multhoff, Gabriele and Mu{\~{n}}oz, Luis Enrique and M{\"{u}}nz, Christian and Nakayama, Toshinori and Nasi, Milena and Neumann, Katrin and Ng, Lai Guan and Niedobitek, Antonia and Nourshargh, Sussan and N{\'{u}}{\~{n}}ez, Gabriel and O'Connor, Jos{\'{e}}-Enrique and Ochel, Aaron and Oja, Anna and Ordonez, Diana and Orfao, Alberto and Orlowski-Oliver, Eva and Ouyang, Wenjun and Oxenius, Annette and Palankar, Raghavendra and Panse, Isabel and Pattanapanyasat, Kovit and Paulsen, Malte and Pavlinic, Dinko and Penter, Livius and Peterson, P{\"{a}}rt and Peth, Christian and Petriz, Jordi and Piancone, Federica and Pickl, Winfried F and Piconese, Silvia and Pinti, Marcello and Pockley, A Graham and Podolska, Malgorzata Justyna and Poon, Zhiyong and Pracht, Katharina and Prinz, Immo and Pucillo, Carlo E M and Quataert, Sally A and Quatrini, Linda and Quinn, Kylie M and Radbruch, Helena and Radstake, Tim R D J and Rahmig, Susann and Rahn, Hans-Peter and Rajwa, Bartek and Ravichandran, Gevitha and Raz, Yotam and Rebhahn, Jonathan A and Recktenwald, Diether and Reimer, Dorothea and e Sousa, Caetano and Remmerswaal, Ester B M and Richter, Lisa and Rico, Laura G and Riddell, Andy and Rieger, Aja M and Robinson, J Paul and Romagnani, Chiara and Rubartelli, Anna and Ruland, J{\"{u}}rgen and Saalm{\"{u}}ller, Armin and Saeys, Yvan and Saito, Takashi and Sakaguchi, Shimon and Sala-de-Oyanguren, Francisco and Samstag, Yvonne and Sanderson, Sharon and Sandrock, Inga and Santoni, Angela and Sanz, Ramon Bellm{\`{a}}s and Saresella, Marina and Sautes-Fridman, Catherine and Sawitzki, Birgit and Schadt, Linda and Scheffold, Alexander and Scherer, Hans U and Schiemann, Matthias and Schildberg, Frank A and Schimisky, Esther and Schlitzer, Andreas and Schlosser, Josephine and Schmid, Stephan and Schmitt, Steffen and Schober, Kilian and Schraivogel, Daniel and Schuh, Wolfgang and Sch{\"{u}}ler, Thomas and Schulte, Reiner and Schulz, Axel Ronald and Schulz, Sebastian R and Scott{\'{a}}, Cristiano and Scott-Algara, Daniel and Sester, David P and Shankey, T Vincent and Silva-Santos, Bruno and Simon, Anna Katharina and Sitnik, Katarzyna M and Sozzani, Silvano and Speiser, Daniel E and Spidlen, Josef and Stahlberg, Anders and Stall, Alan M and Stanley, Natalie and Stark, Regina and Stehle, Christina and Steinmetz, Tobit and Stockinger, Hannes and Takahama, Yousuke and Takeda, Kiyoshi and Tan, Leonard and T{\'{a}}rnok, Attila and Tiegs, Gisa and Toldi, Gergely and Tornack, Julia and Traggiai, Elisabetta and Trebak, Mohamed and Tree, Timothy I M and Trotter, Joe and Trowsdale, John and Tsoumakidou, Maria and Ulrich, Henning and Urbanczyk, Sophia and van de Veen, Willem and van den Broek, Maries and van der Pol, Edwin and {Van Gassen}, Sofie and {Van Isterdael}, Gert and van Lier, Ren{\'{e}} A W and Veldhoen, Marc and Vento-Asturias, Salvador and Vieira, Paulo and Voehringer, David and Volk, Hans-Dieter and von Borstel, Anouk and von Volkmann, Konrad and Waisman, Ari and Walker, Rachael V and Wallace, Paul K and Wang, Sa A and Wang, Xin M and Ward, Michael D and Ward-Hartstonge, Kirsten A and Warnatz, Klaus and Warnes, Gary and Warth, Sarah and Waskow, Claudia and Watson, James V and Watzl, Carsten and Wegener, Leonie and Weisenburger, Thomas and Wiedemann, Annika and Wienands, J{\"{u}}rgen and Wilharm, Anneke and Wilkinson, Robert J and Willimsky, Gerald and Wing, James B and Winkelmann, Rieke and Winkler, Thomas H and Wirz, Oliver F and Wong, Alicia and Wurst, Peter and Yang, Jennie H M and Yang, Juhao and Yazdanbakhsh, Maria and Yu, Liping and Yue, Alice and Zhang, Hanlin and Zhao, Yi and Ziegler, Susanne Maria and Zielinski, Christina and Zimmermann, Jakob and Zychlinsky, Arturo},
doi = {10.1002/eji.201970107},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Cossarizza et al. - 2019 - Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).pdf:pdf},
journal = {European Journal of Immunology},
keywords = {OA,fund{\_}not{\_}ack,original},
mendeley-tags = {OA,fund{\_}not{\_}ack,original},
number = {10},
pages = {1457--1973},
pmid = {31633216},
title = {{Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)}},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.201970107},
volume = {49},
year = {2019}
}
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