Using annotated peptide mass spectrum libraries for protein identification. Craig, R., Cortens, J. C., Fenyo, D., & Beavis, R. C. J Proteome Res, 5(8):1843–1849, 2006. doi abstract bibtex A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. This system was based on the annotated spectra currently available in the Global Proteome Machine Database (GPMDB). The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence modifications, and parent ion charge. The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, resulting in a peptide sequence identification based on scoring the similarity of the experimental spectrum with the contents of the library. A software implementation that performs this type of library search was constructed and successfully used to obtain sequence identifications. The annotated tandem mass spectrum libraries for the Homo sapiens, Mus musculus, and Saccharomyces cerevisiae proteomes and search software were made available for download and use by other groups.
@Article{craig06using,
author = {R. Craig and J. C. Cortens and D. Fenyo and R. C. Beavis},
title = {Using annotated peptide mass spectrum libraries for protein identification.},
journal = {J Proteome Res},
year = {2006},
volume = {5},
number = {8},
pages = {1843--1849},
abstract = {A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. This system was based on the annotated spectra currently available in the Global Proteome Machine Database (GPMDB). The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence modifications, and parent ion charge. The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, resulting in a peptide sequence identification based on scoring the similarity of the experimental spectrum with the contents of the library. A software implementation that performs this type of library search was constructed and successfully used to obtain sequence identifications. The annotated tandem mass spectrum libraries for the Homo sapiens, Mus musculus, and Saccharomyces cerevisiae proteomes and search software were made available for download and use by other groups.},
doi = {10.1021/pr0602085},
keywords = {Amino Acid Sequence; Animals; Databases, Protein; Humans; Information Storage and Retrieval; Mass Spectrometry; Mice; Molecular Sequence Data; Peptide Library; Peptides; Proteins; Reproducibility of Results; Saccharomyces cerevisiae Proteins, chemistry},
optmonth = aug,
owner = {fhufsky},
pmid = {16889405},
timestamp = {2012.03.21},
}
Downloads: 0
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