@techreport{crespo_bioluminescent_2023,
	type = {preprint},
	title = {A {Bioluminescent} {Activity} {Dependent} ({BLADe}) {Platform} for {Converting} {Neuronal} {Activity} to {Photoreceptor} {Activation}},
	url = {http://biorxiv.org/lookup/doi/10.1101/2023.06.25.546469},
	abstract = {Abstract
          
            We developed a platform that utilizes a calcium-dependent luciferase to convert neuronal activity into activation of light sensing domains within the same cell. The platform is based on a
            Gaussia
            luciferase variant with high light emission split by calmodulin-M13 sequences that depends on influx of calcium ions (Ca
            2+
            ) for functional reconstitution. In the presence of its luciferin, coelenterazine (CTZ), Ca
            2+
            influx results in light emission that drives activation of photoreceptors, including optogenetic channels and LOV domains. Critical features of the converter luciferase are light emission low enough to not activate photoreceptors under baseline condition and high enough to activate photosensing elements in the presence of Ca
            2+
            and luciferin. We demonstrate performance of this activity-dependent sensor and integrator for changing membrane potential and driving transcription in individual and populations of neurons
            in vitro
            and
            in vivo
            .},
	language = {en},
	urldate = {2023-12-11},
	institution = {Neuroscience},
	author = {Crespo, Emmanuel L. and Pal, Akash and Prakash, Mansi and Silvagnoli, Alexander D. and Zaidi, Zohair and Gomez-Ramirez, Manuel and Tree, Maya O. and Shaner, Nathan C. and Lipscombe, Diane and Moore, Christopher and Hochgeschwender, Ute},
	month = jun,
	year = {2023},
	doi = {10.1101/2023.06.25.546469},
}

{"_id":"zEPthqAtybCouZkeK","bibbaseid":"crespo-pal-prakash-silvagnoli-zaidi-gomezramirez-tree-shaner-etal-abioluminescentactivitydependentbladeplatformforconvertingneuronalactivitytophotoreceptoractivation-2023","author_short":["Crespo, E. L.","Pal, A.","Prakash, M.","Silvagnoli, A. D.","Zaidi, Z.","Gomez-Ramirez, M.","Tree, M. O.","Shaner, N. C.","Lipscombe, D.","Moore, C.","Hochgeschwender, U."],"bibdata":{"bibtype":"techreport","type":"preprint","title":"A Bioluminescent Activity Dependent (BLADe) Platform for Converting Neuronal Activity to Photoreceptor Activation","url":"http://biorxiv.org/lookup/doi/10.1101/2023.06.25.546469","abstract":"Abstract We developed a platform that utilizes a calcium-dependent luciferase to convert neuronal activity into activation of light sensing domains within the same cell. The platform is based on a Gaussia luciferase variant with high light emission split by calmodulin-M13 sequences that depends on influx of calcium ions (Ca 2+ ) for functional reconstitution. In the presence of its luciferin, coelenterazine (CTZ), Ca 2+ influx results in light emission that drives activation of photoreceptors, including optogenetic channels and LOV domains. Critical features of the converter luciferase are light emission low enough to not activate photoreceptors under baseline condition and high enough to activate photosensing elements in the presence of Ca 2+ and luciferin. We demonstrate performance of this activity-dependent sensor and integrator for changing membrane potential and driving transcription in individual and populations of neurons in vitro and in vivo .","language":"en","urldate":"2023-12-11","institution":"Neuroscience","author":[{"propositions":[],"lastnames":["Crespo"],"firstnames":["Emmanuel","L."],"suffixes":[]},{"propositions":[],"lastnames":["Pal"],"firstnames":["Akash"],"suffixes":[]},{"propositions":[],"lastnames":["Prakash"],"firstnames":["Mansi"],"suffixes":[]},{"propositions":[],"lastnames":["Silvagnoli"],"firstnames":["Alexander","D."],"suffixes":[]},{"propositions":[],"lastnames":["Zaidi"],"firstnames":["Zohair"],"suffixes":[]},{"propositions":[],"lastnames":["Gomez-Ramirez"],"firstnames":["Manuel"],"suffixes":[]},{"propositions":[],"lastnames":["Tree"],"firstnames":["Maya","O."],"suffixes":[]},{"propositions":[],"lastnames":["Shaner"],"firstnames":["Nathan","C."],"suffixes":[]},{"propositions":[],"lastnames":["Lipscombe"],"firstnames":["Diane"],"suffixes":[]},{"propositions":[],"lastnames":["Moore"],"firstnames":["Christopher"],"suffixes":[]},{"propositions":[],"lastnames":["Hochgeschwender"],"firstnames":["Ute"],"suffixes":[]}],"month":"June","year":"2023","doi":"10.1101/2023.06.25.546469","bibtex":"@techreport{crespo_bioluminescent_2023,\n\ttype = {preprint},\n\ttitle = {A {Bioluminescent} {Activity} {Dependent} ({BLADe}) {Platform} for {Converting} {Neuronal} {Activity} to {Photoreceptor} {Activation}},\n\turl = {http://biorxiv.org/lookup/doi/10.1101/2023.06.25.546469},\n\tabstract = {Abstract\n \n We developed a platform that utilizes a calcium-dependent luciferase to convert neuronal activity into activation of light sensing domains within the same cell. The platform is based on a\n Gaussia\n luciferase variant with high light emission split by calmodulin-M13 sequences that depends on influx of calcium ions (Ca\n 2+\n ) for functional reconstitution. In the presence of its luciferin, coelenterazine (CTZ), Ca\n 2+\n influx results in light emission that drives activation of photoreceptors, including optogenetic channels and LOV domains. Critical features of the converter luciferase are light emission low enough to not activate photoreceptors under baseline condition and high enough to activate photosensing elements in the presence of Ca\n 2+\n and luciferin. We demonstrate performance of this activity-dependent sensor and integrator for changing membrane potential and driving transcription in individual and populations of neurons\n in vitro\n and\n in vivo\n .},\n\tlanguage = {en},\n\turldate = {2023-12-11},\n\tinstitution = {Neuroscience},\n\tauthor = {Crespo, Emmanuel L. and Pal, Akash and Prakash, Mansi and Silvagnoli, Alexander D. and Zaidi, Zohair and Gomez-Ramirez, Manuel and Tree, Maya O. and Shaner, Nathan C. and Lipscombe, Diane and Moore, Christopher and Hochgeschwender, Ute},\n\tmonth = jun,\n\tyear = {2023},\n\tdoi = {10.1101/2023.06.25.546469},\n}\n\n","author_short":["Crespo, E. L.","Pal, A.","Prakash, M.","Silvagnoli, A. D.","Zaidi, Z.","Gomez-Ramirez, M.","Tree, M. O.","Shaner, N. C.","Lipscombe, D.","Moore, C.","Hochgeschwender, U."],"key":"crespo_bioluminescent_2023","id":"crespo_bioluminescent_2023","bibbaseid":"crespo-pal-prakash-silvagnoli-zaidi-gomezramirez-tree-shaner-etal-abioluminescentactivitydependentbladeplatformforconvertingneuronalactivitytophotoreceptoractivation-2023","role":"author","urls":{"Paper":"http://biorxiv.org/lookup/doi/10.1101/2023.06.25.546469"},"metadata":{"authorlinks":{}}},"bibtype":"techreport","biburl":"https://api.zotero.org/users/5859248/collections/ZYVZCB4C/items?key=i592Yilg8w9omECWaTAnxNT7&format=bibtex&limit=100","dataSources":["edWworq2CtfaweaC3"],"keywords":[],"search_terms":["bioluminescent","activity","dependent","blade","platform","converting","neuronal","activity","photoreceptor","activation","crespo","pal","prakash","silvagnoli","zaidi","gomez-ramirez","tree","shaner","lipscombe","moore","hochgeschwender"],"title":"A Bioluminescent Activity Dependent (BLADe) Platform for Converting Neuronal Activity to Photoreceptor Activation","year":2023}