Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development. Crespo, E. L., Prakash, M., Bjorefeldt, A., Medendorp, W. E., Shaner, N. C., Lipscombe, D., Moore, C. I., & Hochgeschwender, U. STAR Protocols, 2(3):100667, 2021. ![Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex 8 downloads Summary Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice. For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).
@article{CRESPO2021100667,
title = {Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development},
journal = {STAR Protocols},
volume = {2},
number = {3},
pages = {100667},
year = {2021},
issn = {2666-1667},
doi = {https://doi.org/10.1016/j.xpro.2021.100667},
url = {https://www.sciencedirect.com/science/article/pii/S2666166721003749},
author = {Emmanuel L. Crespo and Mansi Prakash and Andreas Bjorefeldt and William E. Medendorp and Nathan C. Shaner and Diane Lipscombe and Christopher I. Moore and Ute Hochgeschwender},
keywords = {Developmental biology, Microscopy, Model Organisms, Molecular Biology, Neuroscience, Biotechnology and bioengineering},
abstract = {Summary
Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice. For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).}
}
Downloads: 8
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I.","Hochgeschwender, U."],"bibdata":{"bibtype":"article","type":"article","title":"Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development","journal":"STAR Protocols","volume":"2","number":"3","pages":"100667","year":"2021","issn":"2666-1667","doi":"https://doi.org/10.1016/j.xpro.2021.100667","url":"https://www.sciencedirect.com/science/article/pii/S2666166721003749","author":[{"firstnames":["Emmanuel","L."],"propositions":[],"lastnames":["Crespo"],"suffixes":[]},{"firstnames":["Mansi"],"propositions":[],"lastnames":["Prakash"],"suffixes":[]},{"firstnames":["Andreas"],"propositions":[],"lastnames":["Bjorefeldt"],"suffixes":[]},{"firstnames":["William","E."],"propositions":[],"lastnames":["Medendorp"],"suffixes":[]},{"firstnames":["Nathan","C."],"propositions":[],"lastnames":["Shaner"],"suffixes":[]},{"firstnames":["Diane"],"propositions":[],"lastnames":["Lipscombe"],"suffixes":[]},{"firstnames":["Christopher","I."],"propositions":[],"lastnames":["Moore"],"suffixes":[]},{"firstnames":["Ute"],"propositions":[],"lastnames":["Hochgeschwender"],"suffixes":[]}],"keywords":"Developmental biology, Microscopy, Model Organisms, Molecular Biology, Neuroscience, Biotechnology and bioengineering","abstract":"Summary Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice. For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).","bibtex":"@article{CRESPO2021100667,\n\ttitle = {Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development},\n\tjournal = {STAR Protocols},\n\tvolume = {2},\n\tnumber = {3},\n\tpages = {100667},\n\tyear = {2021},\n\tissn = {2666-1667},\n\tdoi = {https://doi.org/10.1016/j.xpro.2021.100667},\n\turl = {https://www.sciencedirect.com/science/article/pii/S2666166721003749},\n\tauthor = {Emmanuel L. Crespo and Mansi Prakash and Andreas Bjorefeldt and William E. Medendorp and Nathan C. Shaner and Diane Lipscombe and Christopher I. Moore and Ute Hochgeschwender},\n\tkeywords = {Developmental biology, Microscopy, Model Organisms, Molecular Biology, Neuroscience, Biotechnology and bioengineering},\n\tabstract = {Summary\n\tBioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice. For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).}\n}\n\n","author_short":["Crespo, E. L.","Prakash, M.","Bjorefeldt, A.","Medendorp, W. E.","Shaner, N. C.","Lipscombe, D.","Moore, C. 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