@article{crespo_bioluminescent_2021,
	title = {Bioluminescent optogenetic ({BL}-{OG}) activation of neurons during mouse postnatal brain development},
	volume = {2},
	issn = {2666-1667},
	url = {https://www.sciencedirect.com/science/article/pii/S2666166721003749},
	doi = {https://doi.org/10.1016/j.xpro.2021.100667},
	abstract = {Summary Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice. For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).},
	number = {3},
	journal = {STAR Protocols},
	author = {Crespo, Emmanuel L. and Prakash, Mansi and Bjorefeldt, Andreas and Medendorp, William E. and Shaner, Nathan C. and Lipscombe, Diane and Moore, Christopher I. and Hochgeschwender, Ute},
	year = {2021},
	keywords = {BiolumHub, Biotechnology and bioengineering, Developmental biology, Microscopy, Model Organisms, Molecular Biology, Neuroscience},
	pages = {100667},
}

{"_id":"zbT3zfYBENSA7sgsA","bibbaseid":"crespo-prakash-bjorefeldt-medendorp-shaner-lipscombe-moore-hochgeschwender-bioluminescentoptogeneticblogactivationofneuronsduringmousepostnatalbraindevelopment-2021","author_short":["Crespo, E. L.","Prakash, M.","Bjorefeldt, A.","Medendorp, W. E.","Shaner, N. C.","Lipscombe, D.","Moore, C. I.","Hochgeschwender, U."],"bibdata":{"bibtype":"article","type":"article","title":"Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development","volume":"2","issn":"2666-1667","url":"https://www.sciencedirect.com/science/article/pii/S2666166721003749","doi":"https://doi.org/10.1016/j.xpro.2021.100667","abstract":"Summary Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice. For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).","number":"3","journal":"STAR Protocols","author":[{"propositions":[],"lastnames":["Crespo"],"firstnames":["Emmanuel","L."],"suffixes":[]},{"propositions":[],"lastnames":["Prakash"],"firstnames":["Mansi"],"suffixes":[]},{"propositions":[],"lastnames":["Bjorefeldt"],"firstnames":["Andreas"],"suffixes":[]},{"propositions":[],"lastnames":["Medendorp"],"firstnames":["William","E."],"suffixes":[]},{"propositions":[],"lastnames":["Shaner"],"firstnames":["Nathan","C."],"suffixes":[]},{"propositions":[],"lastnames":["Lipscombe"],"firstnames":["Diane"],"suffixes":[]},{"propositions":[],"lastnames":["Moore"],"firstnames":["Christopher","I."],"suffixes":[]},{"propositions":[],"lastnames":["Hochgeschwender"],"firstnames":["Ute"],"suffixes":[]}],"year":"2021","keywords":"BiolumHub, Biotechnology and bioengineering, Developmental biology, Microscopy, Model Organisms, Molecular Biology, Neuroscience","pages":"100667","bibtex":"@article{crespo_bioluminescent_2021,\n\ttitle = {Bioluminescent optogenetic ({BL}-{OG}) activation of neurons during mouse postnatal brain development},\n\tvolume = {2},\n\tissn = {2666-1667},\n\turl = {https://www.sciencedirect.com/science/article/pii/S2666166721003749},\n\tdoi = {https://doi.org/10.1016/j.xpro.2021.100667},\n\tabstract = {Summary Bioluminescent optogenetics (BL-OG) allows activation of photosensory proteins, such as opsins, by either fiberoptics or by administering a luciferin. BL-OG thus confers both optogenetic and chemogenetic access within the same genetically targeted neuron. This bimodality offers a powerful approach for non-invasive chemogenetic manipulation of neural activity during brain development and adult behaviors with standard optogenetic spatiotemporal precision. We detail protocols for bioluminescent stimulation of neurons in postnatally developing brain and its validation through bioluminescence imaging and electrophysiological recording in mice. For complete information on the use and execution of this protocol, please refer to Medendorp et al. (2021).},\n\tnumber = {3},\n\tjournal = {STAR Protocols},\n\tauthor = {Crespo, Emmanuel L. and Prakash, Mansi and Bjorefeldt, Andreas and Medendorp, William E. and Shaner, Nathan C. and Lipscombe, Diane and Moore, Christopher I. and Hochgeschwender, Ute},\n\tyear = {2021},\n\tkeywords = {BiolumHub, Biotechnology and bioengineering, Developmental biology, Microscopy, Model Organisms, Molecular Biology, Neuroscience},\n\tpages = {100667},\n}\n\n","author_short":["Crespo, E. L.","Prakash, M.","Bjorefeldt, A.","Medendorp, W. E.","Shaner, N. C.","Lipscombe, D.","Moore, C. I.","Hochgeschwender, U."],"key":"crespo_bioluminescent_2021","id":"crespo_bioluminescent_2021","bibbaseid":"crespo-prakash-bjorefeldt-medendorp-shaner-lipscombe-moore-hochgeschwender-bioluminescentoptogeneticblogactivationofneuronsduringmousepostnatalbraindevelopment-2021","role":"author","urls":{"Paper":"https://www.sciencedirect.com/science/article/pii/S2666166721003749"},"keyword":["BiolumHub","Biotechnology and bioengineering","Developmental biology","Microscopy","Model Organisms","Molecular Biology","Neuroscience"],"metadata":{"authorlinks":{}},"downloads":8},"bibtype":"article","biburl":"https://api.zotero.org/users/5859248/collections/ZYVZCB4C/items?key=i592Yilg8w9omECWaTAnxNT7&format=bibtex&limit=100","dataSources":["TvqYudBJxx7Hha9Hu","edWworq2CtfaweaC3"],"keywords":["biolumhub","biotechnology and bioengineering","developmental biology","microscopy","model organisms","molecular biology","neuroscience"],"search_terms":["bioluminescent","optogenetic","activation","neurons","during","mouse","postnatal","brain","development","crespo","prakash","bjorefeldt","medendorp","shaner","lipscombe","moore","hochgeschwender"],"title":"Bioluminescent optogenetic (BL-OG) activation of neurons during mouse postnatal brain development","year":2021,"downloads":8}