@ARTICLE{Crouch2022-fv,
  title    = "Ensembles of endothelial and mural cells promote angiogenesis in
              prenatal human brain",
  author   = "Crouch, Elizabeth E and Bhaduri, Aparna and Andrews, Madeline G
              and Cebrian-Silla, Arantxa and Diafos, Loukas N and Birrueta,
              Janeth Ochoa and Wedderburn-Pugh, Kaylee and Valenzuela, Edward J
              and Bennett, Neal K and Eze, Ugomma C and Sandoval-Espinosa,
              Carmen and Chen, Jiapei and Mora, Cristina and Ross, Jayden M and
              Howard, Clare E and Gonzalez-Granero, Susana and Lozano, Jaime
              Ferrer and Vento, Maximo and Haeussler, Maximilian and Paredes,
              Mercedes F and Nakamura, Ken and Garcia-Verdugo, Jose Manuel and
              Alvarez-Buylla, Arturo and Kriegstein, Arnold R and Huang, Eric J",
  abstract = "Interactions between angiogenesis and neurogenesis regulate
              embryonic brain development. However, a comprehensive
              understanding of the stages of vascular cell maturation is
              lacking, especially in the prenatal human brain. Using
              fluorescence-activated cell sorting, single-cell transcriptomics,
              and histological and ultrastructural analyses, we show that an
              ensemble of endothelial and mural cell subtypes tile the brain
              vasculature during the second trimester. These vascular cells
              follow distinct developmental trajectories and utilize diverse
              signaling mechanisms, including collagen, laminin, and midkine,
              to facilitate cell-cell communication and maturation.
              Interestingly, our results reveal that tip cells, a subtype of
              endothelial cells, are highly enriched near the ventricular zone,
              the site of active neurogenesis. Consistent with these
              observations, prenatal vascular cells transplanted into cortical
              organoids exhibit restricted lineage potential that favors tip
              cells, promotes neurogenesis, and reduces cellular stress.
              Together, our results uncover important mechanisms into vascular
              maturation during this critical period of human brain
              development.",
  journal  = "Cell",
  volume   =  185,
  number   =  20,
  pages    = "3753--3769.e18",
  month    =  sep,
  year     =  2022,
  address  = "United States",
  keywords = "angiogenesis; arterial endothelial cells; blood brain barrier;
              cortical organoids; endothelial cells; human prenatal brain
              development; mural cells; pericytes; smooth muscle cells; tip
              cells; venous and capillary endothelial cells; ventricular zone",
  language = "en"
}

{"_id":"XJJwSSeHhvN9vqYu4","bibbaseid":"crouch-bhaduri-andrews-cebriansilla-diafos-birrueta-wedderburnpugh-valenzuela-etal-ensemblesofendothelialandmuralcellspromoteangiogenesisinprenatalhumanbrain-2022","author_short":["Crouch, E. E","Bhaduri, A.","Andrews, M. G","Cebrian-Silla, A.","Diafos, L. N","Birrueta, J. O.","Wedderburn-Pugh, K.","Valenzuela, E. J","Bennett, N. K","Eze, U. C","Sandoval-Espinosa, C.","Chen, J.","Mora, C.","Ross, J. M","Howard, C. E","Gonzalez-Granero, S.","Lozano, J. F.","Vento, M.","Haeussler, M.","Paredes, M. F","Nakamura, K.","Garcia-Verdugo, J. M.","Alvarez-Buylla, A.","Kriegstein, A. R","Huang, E. J"],"bibdata":{"bibtype":"article","type":"article","title":"Ensembles of endothelial and mural cells promote angiogenesis in prenatal human brain","author":[{"propositions":[],"lastnames":["Crouch"],"firstnames":["Elizabeth","E"],"suffixes":[]},{"propositions":[],"lastnames":["Bhaduri"],"firstnames":["Aparna"],"suffixes":[]},{"propositions":[],"lastnames":["Andrews"],"firstnames":["Madeline","G"],"suffixes":[]},{"propositions":[],"lastnames":["Cebrian-Silla"],"firstnames":["Arantxa"],"suffixes":[]},{"propositions":[],"lastnames":["Diafos"],"firstnames":["Loukas","N"],"suffixes":[]},{"propositions":[],"lastnames":["Birrueta"],"firstnames":["Janeth","Ochoa"],"suffixes":[]},{"propositions":[],"lastnames":["Wedderburn-Pugh"],"firstnames":["Kaylee"],"suffixes":[]},{"propositions":[],"lastnames":["Valenzuela"],"firstnames":["Edward","J"],"suffixes":[]},{"propositions":[],"lastnames":["Bennett"],"firstnames":["Neal","K"],"suffixes":[]},{"propositions":[],"lastnames":["Eze"],"firstnames":["Ugomma","C"],"suffixes":[]},{"propositions":[],"lastnames":["Sandoval-Espinosa"],"firstnames":["Carmen"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["Jiapei"],"suffixes":[]},{"propositions":[],"lastnames":["Mora"],"firstnames":["Cristina"],"suffixes":[]},{"propositions":[],"lastnames":["Ross"],"firstnames":["Jayden","M"],"suffixes":[]},{"propositions":[],"lastnames":["Howard"],"firstnames":["Clare","E"],"suffixes":[]},{"propositions":[],"lastnames":["Gonzalez-Granero"],"firstnames":["Susana"],"suffixes":[]},{"propositions":[],"lastnames":["Lozano"],"firstnames":["Jaime","Ferrer"],"suffixes":[]},{"propositions":[],"lastnames":["Vento"],"firstnames":["Maximo"],"suffixes":[]},{"propositions":[],"lastnames":["Haeussler"],"firstnames":["Maximilian"],"suffixes":[]},{"propositions":[],"lastnames":["Paredes"],"firstnames":["Mercedes","F"],"suffixes":[]},{"propositions":[],"lastnames":["Nakamura"],"firstnames":["Ken"],"suffixes":[]},{"propositions":[],"lastnames":["Garcia-Verdugo"],"firstnames":["Jose","Manuel"],"suffixes":[]},{"propositions":[],"lastnames":["Alvarez-Buylla"],"firstnames":["Arturo"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["Arnold","R"],"suffixes":[]},{"propositions":[],"lastnames":["Huang"],"firstnames":["Eric","J"],"suffixes":[]}],"abstract":"Interactions between angiogenesis and neurogenesis regulate embryonic brain development. However, a comprehensive understanding of the stages of vascular cell maturation is lacking, especially in the prenatal human brain. Using fluorescence-activated cell sorting, single-cell transcriptomics, and histological and ultrastructural analyses, we show that an ensemble of endothelial and mural cell subtypes tile the brain vasculature during the second trimester. These vascular cells follow distinct developmental trajectories and utilize diverse signaling mechanisms, including collagen, laminin, and midkine, to facilitate cell-cell communication and maturation. Interestingly, our results reveal that tip cells, a subtype of endothelial cells, are highly enriched near the ventricular zone, the site of active neurogenesis. Consistent with these observations, prenatal vascular cells transplanted into cortical organoids exhibit restricted lineage potential that favors tip cells, promotes neurogenesis, and reduces cellular stress. Together, our results uncover important mechanisms into vascular maturation during this critical period of human brain development.","journal":"Cell","volume":"185","number":"20","pages":"3753–3769.e18","month":"September","year":"2022","address":"United States","keywords":"angiogenesis; arterial endothelial cells; blood brain barrier; cortical organoids; endothelial cells; human prenatal brain development; mural cells; pericytes; smooth muscle cells; tip cells; venous and capillary endothelial cells; ventricular zone","language":"en","bibtex":"@ARTICLE{Crouch2022-fv,\n title = \"Ensembles of endothelial and mural cells promote angiogenesis in\n prenatal human brain\",\n author = \"Crouch, Elizabeth E and Bhaduri, Aparna and Andrews, Madeline G\n and Cebrian-Silla, Arantxa and Diafos, Loukas N and Birrueta,\n Janeth Ochoa and Wedderburn-Pugh, Kaylee and Valenzuela, Edward J\n and Bennett, Neal K and Eze, Ugomma C and Sandoval-Espinosa,\n Carmen and Chen, Jiapei and Mora, Cristina and Ross, Jayden M and\n Howard, Clare E and Gonzalez-Granero, Susana and Lozano, Jaime\n Ferrer and Vento, Maximo and Haeussler, Maximilian and Paredes,\n Mercedes F and Nakamura, Ken and Garcia-Verdugo, Jose Manuel and\n Alvarez-Buylla, Arturo and Kriegstein, Arnold R and Huang, Eric J\",\n abstract = \"Interactions between angiogenesis and neurogenesis regulate\n embryonic brain development. However, a comprehensive\n understanding of the stages of vascular cell maturation is\n lacking, especially in the prenatal human brain. Using\n fluorescence-activated cell sorting, single-cell transcriptomics,\n and histological and ultrastructural analyses, we show that an\n ensemble of endothelial and mural cell subtypes tile the brain\n vasculature during the second trimester. These vascular cells\n follow distinct developmental trajectories and utilize diverse\n signaling mechanisms, including collagen, laminin, and midkine,\n to facilitate cell-cell communication and maturation.\n Interestingly, our results reveal that tip cells, a subtype of\n endothelial cells, are highly enriched near the ventricular zone,\n the site of active neurogenesis. Consistent with these\n observations, prenatal vascular cells transplanted into cortical\n organoids exhibit restricted lineage potential that favors tip\n cells, promotes neurogenesis, and reduces cellular stress.\n Together, our results uncover important mechanisms into vascular\n maturation during this critical period of human brain\n development.\",\n journal = \"Cell\",\n volume = 185,\n number = 20,\n pages = \"3753--3769.e18\",\n month = sep,\n year = 2022,\n address = \"United States\",\n keywords = \"angiogenesis; arterial endothelial cells; blood brain barrier;\n cortical organoids; endothelial cells; human prenatal brain\n development; mural cells; pericytes; smooth muscle cells; tip\n cells; venous and capillary endothelial cells; ventricular zone\",\n language = \"en\"\n}\n\n","author_short":["Crouch, E. E","Bhaduri, A.","Andrews, M. G","Cebrian-Silla, A.","Diafos, L. N","Birrueta, J. O.","Wedderburn-Pugh, K.","Valenzuela, E. J","Bennett, N. K","Eze, U. C","Sandoval-Espinosa, C.","Chen, J.","Mora, C.","Ross, J. M","Howard, C. E","Gonzalez-Granero, S.","Lozano, J. F.","Vento, M.","Haeussler, M.","Paredes, M. F","Nakamura, K.","Garcia-Verdugo, J. M.","Alvarez-Buylla, A.","Kriegstein, A. R","Huang, E. J"],"key":"Crouch2022-fv","id":"Crouch2022-fv","bibbaseid":"crouch-bhaduri-andrews-cebriansilla-diafos-birrueta-wedderburnpugh-valenzuela-etal-ensemblesofendothelialandmuralcellspromoteangiogenesisinprenatalhumanbrain-2022","role":"author","urls":{},"keyword":["angiogenesis; arterial endothelial cells; blood brain barrier; cortical organoids; endothelial cells; human prenatal brain development; mural cells; pericytes; smooth muscle cells; tip cells; venous and capillary endothelial cells; ventricular zone"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":["angiogenesis; arterial endothelial cells; blood brain barrier; cortical organoids; endothelial cells; human prenatal brain development; mural cells; pericytes; smooth muscle cells; tip cells; venous and capillary endothelial cells; ventricular zone"],"search_terms":["ensembles","endothelial","mural","cells","promote","angiogenesis","prenatal","human","brain","crouch","bhaduri","andrews","cebrian-silla","diafos","birrueta","wedderburn-pugh","valenzuela","bennett","eze","sandoval-espinosa","chen","mora","ross","howard","gonzalez-granero","lozano","vento","haeussler","paredes","nakamura","garcia-verdugo","alvarez-buylla","kriegstein","huang"],"title":"Ensembles of endothelial and mural cells promote angiogenesis in prenatal human brain","year":2022}