Organelle Separation and Cell Signaling. Da Costa, L. S. & Arnoult, D. Methods in Molecular Biology (Clifton, N.J.), 1557:111–115, 2017. doi abstract bibtex Recent findings indicate that some signaling hubs coalesce at the surfaces of organelles through the accumulation of ubiquitylated components required for the signal transduction. For instance, ubiquitylated components of the NF-κB pathway accumulated at the endoplasmic reticulum while ubiquitylated components of the IRF3 pathway are found at the Golgi apparatus. Here we describe simple methods to observe and assess these ubiquitylated components by immunoblotting using differential centrifugation and in vitro assays.
@article{da_costa_organelle_2017,
title = {Organelle {Separation} and {Cell} {Signaling}},
volume = {1557},
issn = {1940-6029},
doi = {10.1007/978-1-4939-6780-3_11},
abstract = {Recent findings indicate that some signaling hubs coalesce at the surfaces of organelles through the accumulation of ubiquitylated components required for the signal transduction. For instance, ubiquitylated components of the NF-κB pathway accumulated at the endoplasmic reticulum while ubiquitylated components of the IRF3 pathway are found at the Golgi apparatus. Here we describe simple methods to observe and assess these ubiquitylated components by immunoblotting using differential centrifugation and in vitro assays.},
language = {eng},
journal = {Methods in Molecular Biology (Clifton, N.J.)},
author = {Da Costa, Leandro Silva and Arnoult, Damien},
year = {2017},
pmid = {28078587},
keywords = {Cell fractionation, Differential centrifugation, Immunoblotting, Signaling, Ubiquitination},
pages = {111--115},
}
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