Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories. Dallagassa, C., B.; Huergo, L., F.; Stets, M., I.; Pedrosa, F., O.; Souza, E., M.; Cruz, L., M.; Assis, F., E.; Wolf, S.; Volanski, W.; Picheth, G.; Pigatto-Denardi, C., P.; Farah, S., M.; and Fadel-Picheth, C., M. Genetics and Molecular Research, 13(1):716-722, Genetics and Molecular Research, 2014.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories [link]Website  abstract   bibtex   
The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains. Additionally, eight Shigella sonnei isolates were tested and found to be indistinguishable from E. coli by MALDI-TOF MS under the test conditions.
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 title = {Matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis of Escherichia coli categories},
 type = {article},
 year = {2014},
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 keywords = {Commensal,Escherichia coli,MALDI-TOF,Mass fingerprint,Pathotypes},
 pages = {716-722},
 volume = {13},
 websites = {http://www.funpecrp.com.br/gmr/year2014/vol13-1/pdf/gmr2752.pdf,http://dx.doi.org/10.4238/2014.january.29.2},
 publisher = {Genetics and Molecular Research},
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 abstract = {The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully conserved and the variable peaks suggested that MALDI-TOF MS has the potential to distinguish commensal and uropathogenic E. coli strains. Additionally, eight Shigella sonnei isolates were tested and found to be indistinguishable from E. coli by MALDI-TOF MS under the test conditions.},
 bibtype = {article},
 author = {Dallagassa, C. B. and Huergo, L. F. and Stets, M. I. and Pedrosa, F. O. and Souza, E. M. and Cruz, L. M. and Assis, F. E.A. and Wolf, S. and Volanski, W. and Picheth, G. and Pigatto-Denardi, C. P. and Farah, S. M.S.S. and Fadel-Picheth, C. M.T.},
 journal = {Genetics and Molecular Research},
 number = {1}
}
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