@article{
 id = {91b84b2b-1813-3bd8-9bc5-fcdd0f13722a},
 title = {Origin of β-Carotene-Rich Plastoglobuli in Dunaliella bardawil.},
 type = {article},
 year = {2014},
 identifiers = {[object Object]},
 created = {2015-04-08T07:54:34.000Z},
 pages = {2139-56},
 volume = {164},
 websites = {http://www.ncbi.nlm.nih.gov/pubmed/24567188},
 file_attached = {false},
 profile_id = {14c0bef2-6ca7-3c04-b2ac-61e1821d8cd9},
 last_modified = {2015-04-08T21:21:30.000Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {true},
 hidden = {false},
 citation_key = {Davidi2014},
 abstract = {The halotolerant microalgae Dunaliella bardawil accumulates under nitrogen deprivation two types of lipid droplets: plastoglobuli rich in β-carotene (βC-plastoglobuli) and cytoplasmatic lipid droplets (CLDs). We describe the isolation, composition, and origin of these lipid droplets. Plastoglobuli contain β-carotene, phytoene, and galactolipids missing in CLDs. The two preparations contain different lipid-associated proteins: major lipid droplet protein in CLD and the Prorich carotene globule protein in βC-plastoglobuli. The compositions of triglyceride (TAG) molecular species, total fatty acids, and sn-1+3 and sn-2 positions in the two lipid pools are similar, except for a small increase in palmitic acid in plastoglobuli, suggesting a common origin. The formation of CLD TAG precedes that of βC-plastoglobuli, reaching a maximum after 48 h of nitrogen deprivation and then decreasing. Palmitic acid incorporation kinetics indicated that, at early stages of nitrogen deprivation, CLD TAG is synthesized mostly from newly formed fatty acids, whereas in βC-plastoglobuli, a large part of TAG is produced from fatty acids of preformed membrane lipids. Electron microscopic analyses revealed that CLDs adhere to chloroplast envelope membranes concomitant with appearance of small βC-plastoglobuli within the chloroplast. Based on these results, we propose that CLDs in D. bardawil are produced in the endoplasmatic reticulum, whereas βC-plastoglobuli are made, in part, from hydrolysis of chloroplast membrane lipids and in part, by a continual transfer of TAG or fatty acids derived from CLD.},
 bibtype = {article},
 author = {Davidi, Lital and Shimoni, Eyal and Khozin-Goldberg, Inna and Zamir, Ada and Pick, Uri},
 journal = {Plant physiology},
 number = {4}
}