@article{
 title = {Caffeoyl coenzyme A O-methyltransferase down-regulation is associated with modifications in lignin and cell-wall architecture in flax secondary xylem},
 type = {article},
 year = {2009},
 keywords = {CCoAOMT,Cell wall,Flax,Irregular xylem,Lignin,Monolignol},
 pages = {9-19},
 volume = {47},
 id = {ffad9bae-b46f-35dd-af3c-87c5874188d4},
 created = {2016-07-11T13:42:21.000Z},
 file_attached = {true},
 profile_id = {bae88dda-ac8b-323a-8293-ff3f78f068fe},
 last_modified = {2017-03-15T07:30:53.959Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {true},
 hidden = {false},
 private_publication = {false},
 abstract = {Caffeoyl coenzyme A O-methyltransferase (CCoAOMT, EC 2.1.1.104) down-regulated-flax (Linum usitatissimum) plants were generated using an antisense strategy and functionally characterized. Chemical analyses (acetyl bromide and thioacidolysis) revealed that the lignin quantity was reduced and that the Syringyl/Gua??acyl (S/G) lignin monomer ratio was modified in the non-condensed lignin fraction of two independent down-regulated lines. These modifications were associated with altered xylem organization (both lines), reduced cell-wall thickness (one line) and the appearance of an irregular xylem (irx) phenotype (both lines). In addition UV microspectroscopy also indicated that CCoAOMT down-regulation induced changes in xylem cell-wall structure and the lignin fractions. Microscopic examination also suggested that CCoAOMT down-regulation could influence individual xylem cell size and identity. As a first step towards investigating the cellular mechanisms responsible for the unusual structure of flax lignin (G-rich, condensed), recombinant flax CCoAOMT protein was produced and its affinity for different potential substrates evaluated. Results indicated that the preferred substrate was caffeoyl coenzyme A, followed by 5-hydroxyconiferaldehyde suggesting that flax CCoAOMT possesses a small, but probably significant 5??? methylating activity, in addition to a more usual 3??? methylating activity. ?? 2008 Elsevier Masson SAS. All rights reserved.},
 bibtype = {article},
 author = {Day, Arnaud and Neutelings, Godfrey and Nolin, Fr??d??rique and Grec, S??bastien and Habrant, Anouk and Cr??nier, David and Maher, Bouchra and Rolando, Christian and David, H??l??ne and Chabbert, Brigitte and Hawkins, Simon},
 doi = {10.1016/j.plaphy.2008.09.011},
 journal = {Plant Physiology and Biochemistry},
 number = {1}
}

{"_id":"ytpnr7iGsjchFeRSQ","bibbaseid":"day-neutelings-nolin-grec-habrant-crnier-maher-rolando-etal-caffeoylcoenzymeaomethyltransferasedownregulationisassociatedwithmodificationsinligninandcellwallarchitectureinflaxsecondaryxylem-2009","author_short":["Day, A.","Neutelings, G.","Nolin, F.","Grec, S.","Habrant, A.","Cr??nier, D.","Maher, B.","Rolando, C.","David, H.","Chabbert, B.","Hawkins, S."],"bibdata":{"title":"Caffeoyl coenzyme A O-methyltransferase down-regulation is associated with modifications in lignin and cell-wall architecture in flax secondary xylem","type":"article","year":"2009","keywords":"CCoAOMT,Cell wall,Flax,Irregular xylem,Lignin,Monolignol","pages":"9-19","volume":"47","id":"ffad9bae-b46f-35dd-af3c-87c5874188d4","created":"2016-07-11T13:42:21.000Z","file_attached":"true","profile_id":"bae88dda-ac8b-323a-8293-ff3f78f068fe","last_modified":"2017-03-15T07:30:53.959Z","read":false,"starred":false,"authored":"true","confirmed":"true","hidden":false,"private_publication":false,"abstract":"Caffeoyl coenzyme A O-methyltransferase (CCoAOMT, EC 2.1.1.104) down-regulated-flax (Linum usitatissimum) plants were generated using an antisense strategy and functionally characterized. Chemical analyses (acetyl bromide and thioacidolysis) revealed that the lignin quantity was reduced and that the Syringyl/Gua??acyl (S/G) lignin monomer ratio was modified in the non-condensed lignin fraction of two independent down-regulated lines. These modifications were associated with altered xylem organization (both lines), reduced cell-wall thickness (one line) and the appearance of an irregular xylem (irx) phenotype (both lines). In addition UV microspectroscopy also indicated that CCoAOMT down-regulation induced changes in xylem cell-wall structure and the lignin fractions. Microscopic examination also suggested that CCoAOMT down-regulation could influence individual xylem cell size and identity. As a first step towards investigating the cellular mechanisms responsible for the unusual structure of flax lignin (G-rich, condensed), recombinant flax CCoAOMT protein was produced and its affinity for different potential substrates evaluated. Results indicated that the preferred substrate was caffeoyl coenzyme A, followed by 5-hydroxyconiferaldehyde suggesting that flax CCoAOMT possesses a small, but probably significant 5??? methylating activity, in addition to a more usual 3??? methylating activity. ?? 2008 Elsevier Masson SAS. All rights reserved.","bibtype":"article","author":"Day, Arnaud and Neutelings, Godfrey and Nolin, Fr??d??rique and Grec, S??bastien and Habrant, Anouk and Cr??nier, David and Maher, Bouchra and Rolando, Christian and David, H??l??ne and Chabbert, Brigitte and Hawkins, Simon","doi":"10.1016/j.plaphy.2008.09.011","journal":"Plant Physiology and Biochemistry","number":"1","bibtex":"@article{\n title = {Caffeoyl coenzyme A O-methyltransferase down-regulation is associated with modifications in lignin and cell-wall architecture in flax secondary xylem},\n type = {article},\n year = {2009},\n keywords = {CCoAOMT,Cell wall,Flax,Irregular xylem,Lignin,Monolignol},\n pages = {9-19},\n volume = {47},\n id = {ffad9bae-b46f-35dd-af3c-87c5874188d4},\n created = {2016-07-11T13:42:21.000Z},\n file_attached = {true},\n profile_id = {bae88dda-ac8b-323a-8293-ff3f78f068fe},\n last_modified = {2017-03-15T07:30:53.959Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n private_publication = {false},\n abstract = {Caffeoyl coenzyme A O-methyltransferase (CCoAOMT, EC 2.1.1.104) down-regulated-flax (Linum usitatissimum) plants were generated using an antisense strategy and functionally characterized. Chemical analyses (acetyl bromide and thioacidolysis) revealed that the lignin quantity was reduced and that the Syringyl/Gua??acyl (S/G) lignin monomer ratio was modified in the non-condensed lignin fraction of two independent down-regulated lines. These modifications were associated with altered xylem organization (both lines), reduced cell-wall thickness (one line) and the appearance of an irregular xylem (irx) phenotype (both lines). In addition UV microspectroscopy also indicated that CCoAOMT down-regulation induced changes in xylem cell-wall structure and the lignin fractions. Microscopic examination also suggested that CCoAOMT down-regulation could influence individual xylem cell size and identity. As a first step towards investigating the cellular mechanisms responsible for the unusual structure of flax lignin (G-rich, condensed), recombinant flax CCoAOMT protein was produced and its affinity for different potential substrates evaluated. Results indicated that the preferred substrate was caffeoyl coenzyme A, followed by 5-hydroxyconiferaldehyde suggesting that flax CCoAOMT possesses a small, but probably significant 5??? methylating activity, in addition to a more usual 3??? methylating activity. ?? 2008 Elsevier Masson SAS. All rights reserved.},\n bibtype = {article},\n author = {Day, Arnaud and Neutelings, Godfrey and Nolin, Fr??d??rique and Grec, S??bastien and Habrant, Anouk and Cr??nier, David and Maher, Bouchra and Rolando, Christian and David, H??l??ne and Chabbert, Brigitte and Hawkins, Simon},\n doi = {10.1016/j.plaphy.2008.09.011},\n journal = {Plant Physiology and Biochemistry},\n number = {1}\n}","author_short":["Day, A.","Neutelings, G.","Nolin, F.","Grec, S.","Habrant, A.","Cr??nier, D.","Maher, B.","Rolando, C.","David, H.","Chabbert, B.","Hawkins, S."],"urls":{"Paper":"https://bibbase.org/service/mendeley/bae88dda-ac8b-323a-8293-ff3f78f068fe/file/0b192f17-aba6-a8fb-67ae-c998f1058f58/2009-Caffeoyl_coenzyme_A_O-methyltransferase_down-regulation_is_associated_with_modifications_in_lignin_and_cell-wall_ar.pdf.pdf"},"biburl":"https://bibbase.org/service/mendeley/bae88dda-ac8b-323a-8293-ff3f78f068fe","bibbaseid":"day-neutelings-nolin-grec-habrant-crnier-maher-rolando-etal-caffeoylcoenzymeaomethyltransferasedownregulationisassociatedwithmodificationsinligninandcellwallarchitectureinflaxsecondaryxylem-2009","role":"author","keyword":["CCoAOMT","Cell wall","Flax","Irregular xylem","Lignin","Monolignol"],"metadata":{"authorlinks":{}},"downloads":0},"bibtype":"article","biburl":"https://bibbase.org/service/mendeley/bae88dda-ac8b-323a-8293-ff3f78f068fe","dataSources":["2252seNhipfTmjEBQ"],"keywords":["ccoaomt","cell wall","flax","irregular xylem","lignin","monolignol"],"search_terms":["caffeoyl","coenzyme","methyltransferase","down","regulation","associated","modifications","lignin","cell","wall","architecture","flax","secondary","xylem","day","neutelings","nolin","grec","habrant","cr??nier","maher","rolando","david","chabbert","hawkins"],"title":"Caffeoyl coenzyme A O-methyltransferase down-regulation is associated with modifications in lignin and cell-wall architecture in flax secondary xylem","year":2009}