Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression. Delgado-Diaz, D. J., Jesaveluk, B., Hayward, J. A, Tyssen, D., Alisoltani, A., Potgieter, M., Bell, L., Ross, E., Iranzadeh, A., Allali, I., Dabee, S., Barnabas, S., Gamieldien, H., Blackburn, J. M, Mulder, N., Smith, S. B, Edwards, V. L, Burgener, A. D, Bekker, L., Ravel, J., Passmore, J. S, Masson, L., Hearps, A. C, & Tachedjian, G. Microbiome, 10(1):141, Springer Science and Business Media LLC, aug, 2022. doi abstract bibtex Women with a cervicovaginal microbiota dominated by Lactobacillus spp. are at reduced risk of acquiring sexually transmitted infections including HIV, but the biological mechanisms involved remain poorly defined. Here, we performed metaproteomics on vaginal swab samples from young South African women (n = 113) and transcriptomics analysis of cervicovaginal epithelial cell cultures to examine the ability of lactic acid, a metabolite produced by cervicovaginal lactobacilli, to modulate genital epithelial barrier function. Compared to women with Lactobacillus-depleted microbiota, women dominated by vaginal lactobacilli exhibit higher abundance of bacterial lactate dehydrogenase, a key enzyme responsible for lactic acid production, which is independently associated with an increased abundance of epithelial barrier proteins. Physiological concentrations of lactic acid enhance epithelial cell culture barrier integrity and increase intercellular junctional molecule expression. These findings reveal a novel ability of vaginal lactic acid to enhance genital epithelial barrier integrity that may help prevent invasion by sexually transmitted pathogens.
@article{Delgado-Diaz2022,
abstract = {Women with a cervicovaginal microbiota dominated by Lactobacillus spp. are at reduced risk of acquiring sexually transmitted infections including HIV, but the biological mechanisms involved remain poorly defined. Here, we performed metaproteomics on vaginal swab samples from young South African women (n = 113) and transcriptomics analysis of cervicovaginal epithelial cell cultures to examine the ability of lactic acid, a metabolite produced by cervicovaginal lactobacilli, to modulate genital epithelial barrier function. Compared to women with Lactobacillus-depleted microbiota, women dominated by vaginal lactobacilli exhibit higher abundance of bacterial lactate dehydrogenase, a key enzyme responsible for lactic acid production, which is independently associated with an increased abundance of epithelial barrier proteins. Physiological concentrations of lactic acid enhance epithelial cell culture barrier integrity and increase intercellular junctional molecule expression. These findings reveal a novel ability of vaginal lactic acid to enhance genital epithelial barrier integrity that may help prevent invasion by sexually transmitted pathogens.},
author = {Delgado-Diaz, David Jose and Jesaveluk, Brianna and Hayward, Joshua A and Tyssen, David and Alisoltani, Arghavan and Potgieter, Matthys and Bell, Liam and Ross, Elizabeth and Iranzadeh, Arash and Allali, Imane and Dabee, Smritee and Barnabas, Shaun and Gamieldien, Hoyam and Blackburn, Jonathan M and Mulder, Nicola and Smith, Steven B and Edwards, Vonetta L and Burgener, Adam D and Bekker, Linda-Gail and Ravel, Jacques and Passmore, Jo-Ann S and Masson, Lindi and Hearps, Anna C and Tachedjian, Gilda},
doi = {10.1186/S40168-022-01337-5},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Delgado-Diaz et al. - 2022 - Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight.pdf:pdf},
issn = {20492618},
journal = {Microbiome},
keywords = {OA,fund{\_}not{\_}ack,original},
mendeley-tags = {OA,fund{\_}not{\_}ack,original},
month = {aug},
number = {1},
pages = {141},
pmid = {36045402},
publisher = {Springer Science and Business Media LLC},
title = {{Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression}},
volume = {10},
year = {2022}
}
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