Quantitative and time-resolved miRNA pattern of early human T cell activation. Diener, C., Hart, M., Kehl, T., Rheinheimer, S., Ludwig, N., Krammes, L., Pawusch, S., Lenhof, K., Tänzer, T., Schub, D., Sester, M., Walch-Rückheim, B., Keller, A., Lenhof, H., & Meese, E. Nucleic Acids Research, 48(18):10164-10183, 09, 2020.
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Paper doi abstract bibtex
Paper doi abstract bibtex T cells are central to the immune response against various pathogens and cancer cells. Complex networks of transcriptional and post-transcriptional regulators, including microRNAs (miRNAs), coordinate the T cell activation process. Available miRNA datasets, however, do not sufficiently dissolve the dynamic changes of miRNA controlled networks upon T cell activation. Here, we established a quantitative and time-resolved expression pattern for the entire miRNome over a period of 24 h upon human T-cell activation. Based on our time-resolved datasets, we identified central miRNAs and specified common miRNA expression profiles. We found the most prominent quantitative expression changes for miR-155-5p with a range from initially 40 molecules/cell to 1600 molecules/cell upon T-cell activation. We established a comprehensive dynamic regulatory network of both the up- and downstream regulation of miR-155. Upstream, we highlight IRF4 and its complexes with SPI1 and BATF as central for the transcriptional regulation of miR-155. Downstream of miR-155-5p, we verified 17 of its target genes by the time-resolved data recorded after T cell activation. Our data provide comprehensive insights into the range of stimulus induced miRNA abundance changes and lay the ground to identify efficient points of intervention for modifying the T cell response.
@article{10.1093/nar/gkaa788,
author = {Diener, Caroline and Hart, Martin and Kehl, Tim and Rheinheimer, Stefanie and Ludwig, Nicole and Krammes, Lena and Pawusch, Sarah and Lenhof, Kerstin and Tänzer, Tanja and Schub, David and Sester, Martina and Walch-Rückheim, Barbara and Keller, Andreas and Lenhof, Hans-Peter and Meese, Eckart},
title = {Quantitative and time-resolved miRNA pattern of early human T cell activation},
journal = {Nucleic Acids Research},
volume = {48},
number = {18},
pages = {10164-10183},
year = {2020},
month = {09},
abstract = {T cells are central to the immune response against various pathogens and cancer cells. Complex networks of transcriptional and post-transcriptional regulators, including microRNAs (miRNAs), coordinate the T cell activation process. Available miRNA datasets, however, do not sufficiently dissolve the dynamic changes of miRNA controlled networks upon T cell activation. Here, we established a quantitative and time-resolved expression pattern for the entire miRNome over a period of 24 h upon human T-cell activation. Based on our time-resolved datasets, we identified central miRNAs and specified common miRNA expression profiles. We found the most prominent quantitative expression changes for miR-155-5p with a range from initially 40 molecules/cell to 1600 molecules/cell upon T-cell activation. We established a comprehensive dynamic regulatory network of both the up- and downstream regulation of miR-155. Upstream, we highlight IRF4 and its complexes with SPI1 and BATF as central for the transcriptional regulation of miR-155. Downstream of miR-155-5p, we verified 17 of its target genes by the time-resolved data recorded after T cell activation. Our data provide comprehensive insights into the range of stimulus induced miRNA abundance changes and lay the ground to identify efficient points of intervention for modifying the T cell response.},
issn = {0305-1048},
doi = {10.1093/nar/gkaa788},
URL = {https://academic.oup.com/nar/article-pdf/48/18/10164/33856637/gkaa788.pdf},
}Downloads: 0
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Complex networks of transcriptional and post-transcriptional regulators, including microRNAs (miRNAs), coordinate the T cell activation process. Available miRNA datasets, however, do not sufficiently dissolve the dynamic changes of miRNA controlled networks upon T cell activation. Here, we established a quantitative and time-resolved expression pattern for the entire miRNome over a period of 24 h upon human T-cell activation. Based on our time-resolved datasets, we identified central miRNAs and specified common miRNA expression profiles. We found the most prominent quantitative expression changes for miR-155-5p with a range from initially 40 molecules/cell to 1600 molecules/cell upon T-cell activation. We established a comprehensive dynamic regulatory network of both the up- and downstream regulation of miR-155. Upstream, we highlight IRF4 and its complexes with SPI1 and BATF as central for the transcriptional regulation of miR-155. 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