@Article{diguistini09de,
  author    = {Scott Diguistini and Nancy Y Liao and Darren Platt and Gordon Robertson and Michael Seidel and Simon K Chan and T. Roderick Docking and Inanc Birol and Robert A Holt and Martin Hirst and Elaine Mardis and Marco A Marra and Richard C Hamelin and J\"org Bohlmann and Colette Breuil and Steven Jm Jones},
  title     = {De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data.},
  journal   = {Genome Biol},
  year      = {2009},
  volume    = {10},
  number    = {9},
  pages     = {R94},
  abstract  = {Sequencing-by-synthesis technologies can reduce the cost of generating de novo genome assemblies. We report a method for assembling draft genome sequences of eukaryotic organisms that integrates sequence information from different sources, and demonstrate its effectiveness by assembling an approximately 32.5 Mb draft genome sequence for the forest pathogen Grosmannia clavigera, an ascomycete fungus. We also developed a method for assessing draft assemblies using Illumina paired end read data and demonstrate how we are using it to guide future sequence finishing. Our results demonstrate that eukaryotic genome sequences can be accurately assembled by combining Illumina, 454 and Sanger sequence data.},
  doi       = {10.1186/gb-2009-10-9-r94},
  keywords  = {Ascomycota; Fungal Proteins; Genome, Fungal; Genomics; Open Reading Frames; Reproducibility of Results; Sequence Analysis, DNA},
  owner     = {swinter},
  pmid      = {19747388},
  timestamp = {2010.04.09},
}

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J."],"bibdata":{"bibtype":"article","type":"article","author":[{"firstnames":["Scott"],"propositions":[],"lastnames":["Diguistini"],"suffixes":[]},{"firstnames":["Nancy","Y"],"propositions":[],"lastnames":["Liao"],"suffixes":[]},{"firstnames":["Darren"],"propositions":[],"lastnames":["Platt"],"suffixes":[]},{"firstnames":["Gordon"],"propositions":[],"lastnames":["Robertson"],"suffixes":[]},{"firstnames":["Michael"],"propositions":[],"lastnames":["Seidel"],"suffixes":[]},{"firstnames":["Simon","K"],"propositions":[],"lastnames":["Chan"],"suffixes":[]},{"firstnames":["T.","Roderick"],"propositions":[],"lastnames":["Docking"],"suffixes":[]},{"firstnames":["Inanc"],"propositions":[],"lastnames":["Birol"],"suffixes":[]},{"firstnames":["Robert","A"],"propositions":[],"lastnames":["Holt"],"suffixes":[]},{"firstnames":["Martin"],"propositions":[],"lastnames":["Hirst"],"suffixes":[]},{"firstnames":["Elaine"],"propositions":[],"lastnames":["Mardis"],"suffixes":[]},{"firstnames":["Marco","A"],"propositions":[],"lastnames":["Marra"],"suffixes":[]},{"firstnames":["Richard","C"],"propositions":[],"lastnames":["Hamelin"],"suffixes":[]},{"firstnames":["Jörg"],"propositions":[],"lastnames":["Bohlmann"],"suffixes":[]},{"firstnames":["Colette"],"propositions":[],"lastnames":["Breuil"],"suffixes":[]},{"firstnames":["Steven","Jm"],"propositions":[],"lastnames":["Jones"],"suffixes":[]}],"title":"De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data.","journal":"Genome Biol","year":"2009","volume":"10","number":"9","pages":"R94","abstract":"Sequencing-by-synthesis technologies can reduce the cost of generating de novo genome assemblies. We report a method for assembling draft genome sequences of eukaryotic organisms that integrates sequence information from different sources, and demonstrate its effectiveness by assembling an approximately 32.5 Mb draft genome sequence for the forest pathogen Grosmannia clavigera, an ascomycete fungus. We also developed a method for assessing draft assemblies using Illumina paired end read data and demonstrate how we are using it to guide future sequence finishing. Our results demonstrate that eukaryotic genome sequences can be accurately assembled by combining Illumina, 454 and Sanger sequence data.","doi":"10.1186/gb-2009-10-9-r94","keywords":"Ascomycota; Fungal Proteins; Genome, Fungal; Genomics; Open Reading Frames; Reproducibility of Results; Sequence Analysis, DNA","owner":"swinter","pmid":"19747388","timestamp":"2010.04.09","bibtex":"@Article{diguistini09de,\n author = {Scott Diguistini and Nancy Y Liao and Darren Platt and Gordon Robertson and Michael Seidel and Simon K Chan and T. Roderick Docking and Inanc Birol and Robert A Holt and Martin Hirst and Elaine Mardis and Marco A Marra and Richard C Hamelin and J\\\"org Bohlmann and Colette Breuil and Steven Jm Jones},\n title = {De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data.},\n journal = {Genome Biol},\n year = {2009},\n volume = {10},\n number = {9},\n pages = {R94},\n abstract = {Sequencing-by-synthesis technologies can reduce the cost of generating de novo genome assemblies. We report a method for assembling draft genome sequences of eukaryotic organisms that integrates sequence information from different sources, and demonstrate its effectiveness by assembling an approximately 32.5 Mb draft genome sequence for the forest pathogen Grosmannia clavigera, an ascomycete fungus. We also developed a method for assessing draft assemblies using Illumina paired end read data and demonstrate how we are using it to guide future sequence finishing. 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