@incollection{Dinkele2023,
abstract = {Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), is the leading infectious killer globally, claiming 1.4 million lives annually. TB control is heavily predicated on treatment of active disease. However, delayed and missed diagnoses, and the six-month duration of standard chemotherapy, contribute to failure of this approach to control the TB epidemic. Enumeration of viable aerosolized Mtb via microbiological culture is complicated by the semi-quantitative nature of "time to positivity" in liquid culture and the slow formation of Colony Forming Units on solid media (four to eight weeks for colonies to become visible). A key motivation informing the development of the RASC platform was the need to capture live, aerosol-derived Mtb for analysis and propagation as part of a larger research program in TB transmission and Mtb aerobiology. Fluorescence microscopy of bioaerosol samples enabled the detection of putative live Mtb in 90{\%} of GeneXpert-confirmed TB patients.},
author = {Dinkele, Ryan and Gessner, Sophia and McKerry, Andrea and Leonard, Bryan and Seldon, Ronnett and Koch, Anastasia S and Morrow, Carl and Gqada, Melitta and Kamariza, Mireille and Bertozzi, Carolyn R and Smith, Brian and McLoud, Courtney and Kamholz, Andrew and Bryden, Wayne and Call, Charles and Kaplan, Gilla and Mizrahi, Valerie and Wood, Robin and Warner, Digby F},
booktitle = {Advances in Medical Imaging, Detection, and Diagnosis},
doi = {10.1201/9781003298038-32},
isbn = {9781003298038},
keywords = {book{\_}chap,original},
mendeley-tags = {book{\_}chap,original},
month = {oct},
pages = {935--954},
publisher = {Jenny Stanford Publishing},
title = {{Capture and visualization of live \textit{Mycobacterium tuberculosis} bacilli from tuberculosis patient bioaerosols}},
url = {https://www.taylorfrancis.com/chapters/edit/10.1201/9781003298038-32/capture-visualization-live-mycobacterium-tuberculosis-bacilli-tuberculosis-patient-bioaerosols-ryan-dinkele-sophia-gessner-andrea-mckerry-bryan-leonard-ronnett-seldon-anastasia-koch-carl},
year = {2023}
}

{"_id":"dRXwvvdiJrrSN8LN9","bibbaseid":"dinkele-gessner-mckerry-leonard-seldon-koch-morrow-gqada-etal-captureandvisualizationofliveimycobacteriumtuberculosisibacillifromtuberculosispatientbioaerosols-2023","author_short":["Dinkele, R.","Gessner, S.","McKerry, A.","Leonard, B.","Seldon, R.","Koch, A. S","Morrow, C.","Gqada, M.","Kamariza, M.","Bertozzi, C. R","Smith, B.","McLoud, C.","Kamholz, A.","Bryden, W.","Call, C.","Kaplan, G.","Mizrahi, V.","Wood, R.","Warner, D. F"],"bibdata":{"bibtype":"incollection","type":"incollection","abstract":"Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), is the leading infectious killer globally, claiming 1.4 million lives annually. TB control is heavily predicated on treatment of active disease. However, delayed and missed diagnoses, and the six-month duration of standard chemotherapy, contribute to failure of this approach to control the TB epidemic. Enumeration of viable aerosolized Mtb via microbiological culture is complicated by the semi-quantitative nature of \"time to positivity\" in liquid culture and the slow formation of Colony Forming Units on solid media (four to eight weeks for colonies to become visible). A key motivation informing the development of the RASC platform was the need to capture live, aerosol-derived Mtb for analysis and propagation as part of a larger research program in TB transmission and Mtb aerobiology. Fluorescence microscopy of bioaerosol samples enabled the detection of putative live Mtb in 90% of GeneXpert-confirmed TB patients.","author":[{"propositions":[],"lastnames":["Dinkele"],"firstnames":["Ryan"],"suffixes":[]},{"propositions":[],"lastnames":["Gessner"],"firstnames":["Sophia"],"suffixes":[]},{"propositions":[],"lastnames":["McKerry"],"firstnames":["Andrea"],"suffixes":[]},{"propositions":[],"lastnames":["Leonard"],"firstnames":["Bryan"],"suffixes":[]},{"propositions":[],"lastnames":["Seldon"],"firstnames":["Ronnett"],"suffixes":[]},{"propositions":[],"lastnames":["Koch"],"firstnames":["Anastasia","S"],"suffixes":[]},{"propositions":[],"lastnames":["Morrow"],"firstnames":["Carl"],"suffixes":[]},{"propositions":[],"lastnames":["Gqada"],"firstnames":["Melitta"],"suffixes":[]},{"propositions":[],"lastnames":["Kamariza"],"firstnames":["Mireille"],"suffixes":[]},{"propositions":[],"lastnames":["Bertozzi"],"firstnames":["Carolyn","R"],"suffixes":[]},{"propositions":[],"lastnames":["Smith"],"firstnames":["Brian"],"suffixes":[]},{"propositions":[],"lastnames":["McLoud"],"firstnames":["Courtney"],"suffixes":[]},{"propositions":[],"lastnames":["Kamholz"],"firstnames":["Andrew"],"suffixes":[]},{"propositions":[],"lastnames":["Bryden"],"firstnames":["Wayne"],"suffixes":[]},{"propositions":[],"lastnames":["Call"],"firstnames":["Charles"],"suffixes":[]},{"propositions":[],"lastnames":["Kaplan"],"firstnames":["Gilla"],"suffixes":[]},{"propositions":[],"lastnames":["Mizrahi"],"firstnames":["Valerie"],"suffixes":[]},{"propositions":[],"lastnames":["Wood"],"firstnames":["Robin"],"suffixes":[]},{"propositions":[],"lastnames":["Warner"],"firstnames":["Digby","F"],"suffixes":[]}],"booktitle":"Advances in Medical Imaging, Detection, and Diagnosis","doi":"10.1201/9781003298038-32","isbn":"9781003298038","keywords":"book_chap,original","mendeley-tags":"book_chap,original","month":"oct","pages":"935–954","publisher":"Jenny Stanford Publishing","title":"Capture and visualization of live <i>Mycobacterium tuberculosis</i> bacilli from tuberculosis patient bioaerosols","url":"https://www.taylorfrancis.com/chapters/edit/10.1201/9781003298038-32/capture-visualization-live-mycobacterium-tuberculosis-bacilli-tuberculosis-patient-bioaerosols-ryan-dinkele-sophia-gessner-andrea-mckerry-bryan-leonard-ronnett-seldon-anastasia-koch-carl","year":"2023","bibtex":"@incollection{Dinkele2023,\r\nabstract = {Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), is the leading infectious killer globally, claiming 1.4 million lives annually. TB control is heavily predicated on treatment of active disease. However, delayed and missed diagnoses, and the six-month duration of standard chemotherapy, contribute to failure of this approach to control the TB epidemic. Enumeration of viable aerosolized Mtb via microbiological culture is complicated by the semi-quantitative nature of \"time to positivity\" in liquid culture and the slow formation of Colony Forming Units on solid media (four to eight weeks for colonies to become visible). A key motivation informing the development of the RASC platform was the need to capture live, aerosol-derived Mtb for analysis and propagation as part of a larger research program in TB transmission and Mtb aerobiology. Fluorescence microscopy of bioaerosol samples enabled the detection of putative live Mtb in 90{\\%} of GeneXpert-confirmed TB patients.},\r\nauthor = {Dinkele, Ryan and Gessner, Sophia and McKerry, Andrea and Leonard, Bryan and Seldon, Ronnett and Koch, Anastasia S and Morrow, Carl and Gqada, Melitta and Kamariza, Mireille and Bertozzi, Carolyn R and Smith, Brian and McLoud, Courtney and Kamholz, Andrew and Bryden, Wayne and Call, Charles and Kaplan, Gilla and Mizrahi, Valerie and Wood, Robin and Warner, Digby F},\r\nbooktitle = {Advances in Medical Imaging, Detection, and Diagnosis},\r\ndoi = {10.1201/9781003298038-32},\r\nisbn = {9781003298038},\r\nkeywords = {book{\\_}chap,original},\r\nmendeley-tags = {book{\\_}chap,original},\r\nmonth = {oct},\r\npages = {935--954},\r\npublisher = {Jenny Stanford Publishing},\r\ntitle = {{Capture and visualization of live \\textit{Mycobacterium tuberculosis} bacilli from tuberculosis patient bioaerosols}},\r\nurl = {https://www.taylorfrancis.com/chapters/edit/10.1201/9781003298038-32/capture-visualization-live-mycobacterium-tuberculosis-bacilli-tuberculosis-patient-bioaerosols-ryan-dinkele-sophia-gessner-andrea-mckerry-bryan-leonard-ronnett-seldon-anastasia-koch-carl},\r\nyear = {2023}\r\n}\r\n","author_short":["Dinkele, R.","Gessner, S.","McKerry, A.","Leonard, B.","Seldon, R.","Koch, A. S","Morrow, C.","Gqada, M.","Kamariza, M.","Bertozzi, C. R","Smith, B.","McLoud, C.","Kamholz, A.","Bryden, W.","Call, C.","Kaplan, G.","Mizrahi, V.","Wood, R.","Warner, D. F"],"key":"Dinkele2023","id":"Dinkele2023","bibbaseid":"dinkele-gessner-mckerry-leonard-seldon-koch-morrow-gqada-etal-captureandvisualizationofliveimycobacteriumtuberculosisibacillifromtuberculosispatientbioaerosols-2023","role":"author","urls":{"Paper":"https://www.taylorfrancis.com/chapters/edit/10.1201/9781003298038-32/capture-visualization-live-mycobacterium-tuberculosis-bacilli-tuberculosis-patient-bioaerosols-ryan-dinkele-sophia-gessner-andrea-mckerry-bryan-leonard-ronnett-seldon-anastasia-koch-carl"},"keyword":["book_chap","original"],"metadata":{"authorlinks":{}}},"bibtype":"incollection","biburl":"https://drive.google.com/uc?export=download&id=1-JLqZ7RwZ3VC2d6ErLGHAtOeMRS_7GCz","dataSources":["Krmt6gt9ktB2s6ARh"],"keywords":["book_chap","original"],"search_terms":["capture","visualization","live","mycobacterium","tuberculosis","bacilli","tuberculosis","patient","bioaerosols","dinkele","gessner","mckerry","leonard","seldon","koch","morrow","gqada","kamariza","bertozzi","smith","mcloud","kamholz","bryden","call","kaplan","mizrahi","wood","warner"],"title":"Capture and visualization of live <i>Mycobacterium tuberculosis</i> bacilli from tuberculosis patient bioaerosols","year":2023}