Ultrastructural evidence for the axonal localization of caudodorsal cell hormone mRNA in the central nervous system of the molluscLymnaea stagnalis. Dirks, R. W.; van Dorp, A. G. M.; van Minnen, J.; Fransen, J. A. M.; van der Ploeg, M.; and Raap, A. K. Microscopy Research and Technique, 25(1):12–18, Wiley Online Library, may, 1993.
Ultrastructural evidence for the axonal localization of caudodorsal cell hormone mRNA in the central nervous system of the molluscLymnaea stagnalis [link]Paper  doi  abstract   bibtex   
The technique of in situ hybridization has been used to evaluate the expression of an ovulation hormone mRNA (caudodorsal cell hormone; CDCH) in the central nervous system (CNS) of the mollusc Lymnaea stagnalis. Hybridization with radioactive as well as with nonradioactive labeled oligonucleotide and plasmid probes revealed a specific labeling on cell bodies of caudodorsal cells (CDCs), which are known to produce CDCH, on the light microscopical level. In addition, specific labeling was observed outside the cell bodies, as far as the cerebral commissure, where CDCH is released in the haemolymph. To investigate whether these signals represent an axonal localization of the CDCH mRNA, we performed in situ hybridization at the electron microscopical (EM) level. The results showed an intraaxonal localization of CDCH mRNA with digoxigenin labeled oligonucleotide and plasmid probes. Gold labeling was observed in secretion granules, and double labeling experiments showed that these granules also contain CDCH. This specific intragranular localization suggest that CDCH mRNA is transported through the axon and released by exocytosis in the haemolymph. \textcopyright 1993 Wiley‐Liss, Inc. Copyright \textcopyright 1993 Wiley‐Liss, Inc.
@article{pop00115,
abstract = {The technique of in situ hybridization has been used to evaluate the expression of an ovulation hormone mRNA (caudodorsal cell hormone; CDCH) in the central nervous system (CNS) of the mollusc Lymnaea stagnalis. Hybridization with radioactive as well as with nonradioactive labeled oligonucleotide and plasmid probes revealed a specific labeling on cell bodies of caudodorsal cells (CDCs), which are known to produce CDCH, on the light microscopical level. In addition, specific labeling was observed outside the cell bodies, as far as the cerebral commissure, where CDCH is released in the haemolymph. To investigate whether these signals represent an axonal localization of the CDCH mRNA, we performed in situ hybridization at the electron microscopical (EM) level. The results showed an intraaxonal localization of CDCH mRNA with digoxigenin labeled oligonucleotide and plasmid probes. Gold labeling was observed in secretion granules, and double labeling experiments showed that these granules also contain CDCH. This specific intragranular localization suggest that CDCH mRNA is transported through the axon and released by exocytosis in the haemolymph. {\textcopyright} 1993 Wiley‐Liss, Inc. Copyright {\textcopyright} 1993 Wiley‐Liss, Inc.},
annote = {Query date: 2020-06-29 13:05:30},
author = {Dirks, Roeland W. and van Dorp, Annette G. M. and van Minnen, Jan and Fransen, Jack A. M. and van der Ploeg, Mels and Raap, Anton K.},
doi = {10.1002/jemt.1070250104},
issn = {1059-910X},
journal = {Microscopy Research and Technique},
keywords = {Cryosections,Digoxigenin,Electron microscopy,In situ hybridization},
month = {may},
number = {1},
pages = {12--18},
publisher = {Wiley Online Library},
title = {{Ultrastructural evidence for the axonal localization of caudodorsal cell hormone mRNA in the central nervous system of the molluscLymnaea stagnalis}},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.1070250104 http://doi.wiley.com/10.1002/jemt.1070250104},
volume = {25},
year = {1993}
}
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