Identification and expression analysis of genes encoding putative cellulose synthases (CesA) in the hybrid aspen, Populus tremula (L.) x P-tremuloides (Michx.). Djerbi, S., Aspeborg, H., Nilsson, P., Sundberg, B., Mellerowicz, E., Blomqvist, K., & Teeri, T. T. Cellulose, 11(3-4):301–312, December, 2004. Place: Dordrecht Publisher: Springer WOS:000225573000004doi abstract bibtex Cellulose is synthesized in plant cell walls by large membrane-bound protein complexes proposed to contain several copies of the catalytic subunit of the cellulose synthase, CesA. Here we report identification of 10 distinct CesA genes within a database of 100,000 ESTs of the hybrid aspen, Populus tremula (L.) x P. tremuloides (Michx.). Expression analyses in normal wood undergoing xylogenesis and in tension wood indicate xylem specific expression of four putative CesA isoenzymes, PttCesA1, PttCesA3-1, PttCesA3-2 and PttCesA9. Both the protein sequences and the expression profiles of PttCesA3-1 and PttCesA3-2 are very similar, and they may thus represent redundant copies of an enzyme with essentially the same function. Further, one of the generally more constitutively expressed CesA genes, PttCesA2, seems to be activated on the opposite side of a tension wood induced stem, while PttCesA6 appears to be more specific for leaf tissues. The rest of the hybrid aspen CesA genes were found to be relatively evenly expressed over the poplar tissues hereby studied.
@article{djerbi_identification_2004,
title = {Identification and expression analysis of genes encoding putative cellulose synthases ({CesA}) in the hybrid aspen, {Populus} tremula ({L}.) x {P}-tremuloides ({Michx}.)},
volume = {11},
issn = {0969-0239},
doi = {10/bc795m},
abstract = {Cellulose is synthesized in plant cell walls by large membrane-bound protein complexes proposed to contain several copies of the catalytic subunit of the cellulose synthase, CesA. Here we report identification of 10 distinct CesA genes within a database of 100,000 ESTs of the hybrid aspen, Populus tremula (L.) x P. tremuloides (Michx.). Expression analyses in normal wood undergoing xylogenesis and in tension wood indicate xylem specific expression of four putative CesA isoenzymes, PttCesA1, PttCesA3-1, PttCesA3-2 and PttCesA9. Both the protein sequences and the expression profiles of PttCesA3-1 and PttCesA3-2 are very similar, and they may thus represent redundant copies of an enzyme with essentially the same function. Further, one of the generally more constitutively expressed CesA genes, PttCesA2, seems to be activated on the opposite side of a tension wood induced stem, while PttCesA6 appears to be more specific for leaf tissues. The rest of the hybrid aspen CesA genes were found to be relatively evenly expressed over the poplar tissues hereby studied.},
language = {English},
number = {3-4},
journal = {Cellulose},
author = {Djerbi, S. and Aspeborg, H. and Nilsson, P. and Sundberg, B. and Mellerowicz, E. and Blomqvist, K. and Teeri, T. T.},
month = dec,
year = {2004},
note = {Place: Dordrecht
Publisher: Springer
WOS:000225573000004},
keywords = {arabidopsis, biosynthesis, catalytic subunits, cellulose synthesis, expression profiling, glycosyltransferases, higher-plants, hybrid aspen, isoxaben, plant cell wall, resistance, sequence-analysis, tension, tension wood, xylem},
pages = {301--312},
}
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Expression analyses in normal wood undergoing xylogenesis and in tension wood indicate xylem specific expression of four putative CesA isoenzymes, PttCesA1, PttCesA3-1, PttCesA3-2 and PttCesA9. Both the protein sequences and the expression profiles of PttCesA3-1 and PttCesA3-2 are very similar, and they may thus represent redundant copies of an enzyme with essentially the same function. Further, one of the generally more constitutively expressed CesA genes, PttCesA2, seems to be activated on the opposite side of a tension wood induced stem, while PttCesA6 appears to be more specific for leaf tissues. 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