Identification of aromatic cytokinins in suspension cultured photoautotrophic cells of Chenopodium rubrum by capillary liquid chromatography/frit – fast atom bombardment mass spectrometry. Doležal, K., Åstot, C., Hanuš, J., Holub, J., Peters, W., Beck, E., Strnad, M., & Sandberg, G. Plant Growth Regulation, 36(2):181–189, February, 2002. Paper doi abstract bibtex Two previously unrecorded endogenous cytokinin metabolites,6-[2-(β-D-glucopyranosyloxy)benzylamino]purine and6-[2-(β-D-glucopyranosyloxy)benzylamino]-2-methylthiopurine, wereidentified, together with 6-benzylamino-9-β-D-glucopyranosylpurine(BAP9G),from Chenopodium rubrum cells, grown autotrophically insuspension culture. The new metabolites belong to the aromatic class ofcytokinins, in which an aromatic side chain is attached at theN6-position of the adenine species. The identification was performedby capillary-liquid chromatography/frit-fast atom bombardment - massspectrometry (LC/FAB MS) after pre-column derivatisation and the structuralelucidation was confirmed by organic synthesis. Cytokinin activity of thecompounds was tested in an Amaranthus bioassay. Theendogenous synthesis of the identified compounds was verified byin vivo deuterium labelling of the analysed cytokininspecies, thereby for the first time providing absolute evidence for theendogenous origin of these compounds.
@article{dolezal_identification_2002,
title = {Identification of aromatic cytokinins in suspension cultured photoautotrophic cells of {Chenopodium} rubrum by capillary liquid chromatography/frit – fast atom bombardment mass spectrometry},
volume = {36},
issn = {1573-5087},
url = {https://doi.org/10.1023/A:1015027906046},
doi = {10/bnfzdw},
abstract = {Two previously unrecorded endogenous cytokinin metabolites,6-[2-(β-D-glucopyranosyloxy)benzylamino]purine and6-[2-(β-D-glucopyranosyloxy)benzylamino]-2-methylthiopurine, wereidentified, together with 6-benzylamino-9-β-D-glucopyranosylpurine(BAP9G),from Chenopodium rubrum cells, grown autotrophically insuspension culture. The new metabolites belong to the aromatic class ofcytokinins, in which an aromatic side chain is attached at theN6-position of the adenine species. The identification was performedby capillary-liquid chromatography/frit-fast atom bombardment - massspectrometry (LC/FAB MS) after pre-column derivatisation and the structuralelucidation was confirmed by organic synthesis. Cytokinin activity of thecompounds was tested in an Amaranthus bioassay. Theendogenous synthesis of the identified compounds was verified byin vivo deuterium labelling of the analysed cytokininspecies, thereby for the first time providing absolute evidence for theendogenous origin of these compounds.},
language = {en},
number = {2},
urldate = {2021-10-19},
journal = {Plant Growth Regulation},
author = {Doležal, Karel and Åstot, Crister and Hanuš, Jan and Holub, Jan and Peters, Wilfried and Beck, Erwin and Strnad, Miroslav and Sandberg, Göran},
month = feb,
year = {2002},
pages = {181--189},
}
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The new metabolites belong to the aromatic class ofcytokinins, in which an aromatic side chain is attached at theN6-position of the adenine species. The identification was performedby capillary-liquid chromatography/frit-fast atom bombardment - massspectrometry (LC/FAB MS) after pre-column derivatisation and the structuralelucidation was confirmed by organic synthesis. Cytokinin activity of thecompounds was tested in an Amaranthus bioassay. Theendogenous synthesis of the identified compounds was verified byin vivo deuterium labelling of the analysed cytokininspecies, thereby for the first time providing absolute evidence for theendogenous origin of these compounds.","language":"en","number":"2","urldate":"2021-10-19","journal":"Plant Growth Regulation","author":[{"propositions":[],"lastnames":["Doležal"],"firstnames":["Karel"],"suffixes":[]},{"propositions":[],"lastnames":["Åstot"],"firstnames":["Crister"],"suffixes":[]},{"propositions":[],"lastnames":["Hanuš"],"firstnames":["Jan"],"suffixes":[]},{"propositions":[],"lastnames":["Holub"],"firstnames":["Jan"],"suffixes":[]},{"propositions":[],"lastnames":["Peters"],"firstnames":["Wilfried"],"suffixes":[]},{"propositions":[],"lastnames":["Beck"],"firstnames":["Erwin"],"suffixes":[]},{"propositions":[],"lastnames":["Strnad"],"firstnames":["Miroslav"],"suffixes":[]},{"propositions":[],"lastnames":["Sandberg"],"firstnames":["Göran"],"suffixes":[]}],"month":"February","year":"2002","pages":"181–189","bibtex":"@article{dolezal_identification_2002,\n\ttitle = {Identification of aromatic cytokinins in suspension cultured photoautotrophic cells of {Chenopodium} rubrum by capillary liquid chromatography/frit – fast atom bombardment mass spectrometry},\n\tvolume = {36},\n\tissn = {1573-5087},\n\turl = {https://doi.org/10.1023/A:1015027906046},\n\tdoi = {10/bnfzdw},\n\tabstract = {Two previously unrecorded endogenous cytokinin metabolites,6-[2-(β-D-glucopyranosyloxy)benzylamino]purine and6-[2-(β-D-glucopyranosyloxy)benzylamino]-2-methylthiopurine, wereidentified, together with 6-benzylamino-9-β-D-glucopyranosylpurine(BAP9G),from Chenopodium rubrum cells, grown autotrophically insuspension culture. The new metabolites belong to the aromatic class ofcytokinins, in which an aromatic side chain is attached at theN6-position of the adenine species. The identification was performedby capillary-liquid chromatography/frit-fast atom bombardment - massspectrometry (LC/FAB MS) after pre-column derivatisation and the structuralelucidation was confirmed by organic synthesis. Cytokinin activity of thecompounds was tested in an Amaranthus bioassay. 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