Separation of Macrophages and Fibroblasts Using Contactless Dielectrophoresis and a Novel ImageJ Macro

Separation of Macrophages and Fibroblasts Using Contactless Dielectrophoresis and a Novel ImageJ Macro. Douglas, T. A., Alinezhadbalalami, N., Balani, N., Schmelz, E. M., & Davalos, R. V. Bioelectricity, 1(1):49-55, 2019. 2576-3113 Douglas, Temple Anne Alinezhadbalalami, Nastaran Balani, Nikita Schmelz, Eva M Davalos, Rafael V R21 CA173092/CA/NCI NIH HHS/United States Journal Article United States 2020/04/16 Bioelectricity. 2019 Mar 1;1(1):49-55. doi: 10.1089/bioe.2018.0004. Epub 2019 Mar 18.
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Background: This study presents a label-free method of separating macrophages and fibroblasts, cell types critically associated with tumors. Materials and Methods: Contactless dielectrophoresis (DEP) devices were used to separate fibroblasts from macrophages by selectively trapping one population. An ImageJ macro was developed to determine the percentage of each population moving or stationary at a given point in time in a video. Results: At 350V(rms), 20 kHz, and 1.25 μL/min, more than 90% of fibroblasts were trapped while less than 20% of macrophages were trapped. Conclusions: Contactless DEP was used to study macrophage and fibroblast separation as a proof-of-concept study for separating cells in the tumor microenvironment. The associated ImageJ macro could be used in other microfluidic cell separation studies.

@article{RN134,
   author = {Douglas, T. A. and Alinezhadbalalami, N. and Balani, N. and Schmelz, E. M. and Davalos, R. V.},
   title = {Separation of Macrophages and Fibroblasts Using Contactless Dielectrophoresis and a Novel ImageJ Macro},
   journal = {Bioelectricity},
   volume = {1},
   number = {1},
   pages = {49-55},
   note = {2576-3113
Douglas, Temple Anne
Alinezhadbalalami, Nastaran
Balani, Nikita
Schmelz, Eva M
Davalos, Rafael V
R21 CA173092/CA/NCI NIH HHS/United States
Journal Article
United States
2020/04/16
Bioelectricity. 2019 Mar 1;1(1):49-55. doi: 10.1089/bioe.2018.0004. Epub 2019 Mar 18.},
   abstract = {Background: This study presents a label-free method of separating macrophages and fibroblasts, cell types critically associated with tumors. Materials and Methods: Contactless dielectrophoresis (DEP) devices were used to separate fibroblasts from macrophages by selectively trapping one population. An ImageJ macro was developed to determine the percentage of each population moving or stationary at a given point in time in a video. Results: At 350V(rms), 20 kHz, and 1.25 μL/min, more than 90% of fibroblasts were trapped while less than 20% of macrophages were trapped. Conclusions: Contactless DEP was used to study macrophage and fibroblast separation as a proof-of-concept study for separating cells in the tumor microenvironment. The associated ImageJ macro could be used in other microfluidic cell separation studies.},
   keywords = {cell separation
microenvironment
microfluidics},
   ISSN = {2576-3105 (Print)
2576-3105},
   DOI = {10.1089/bioe.2018.0004},
   year = {2019},
   type = {Journal Article}
}

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