An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana. Doyle, S. M., Haeger, A., Vain, T., Rigal, A., Viotti, C., Langowska, M., Ma, Q., Friml, J., Raikhel, N. V., Hicks, G. R., & Robert, S. Proc Natl Acad Sci U S A, 112(7):E806–15, February, 2015. Edition: 2015/02/04![An early secretory pathway mediated by GNOM-LIKE 1 and GNOM is essential for basal polarity establishment in Arabidopsis thaliana [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Spatial regulation of the plant hormone indole-3-acetic acid (IAA, or auxin) is essential for plant development. Auxin gradient establishment is mediated by polarly localized auxin transporters, including PIN-FORMED (PIN) proteins. Their localization and abundance at the plasma membrane are tightly regulated by endomembrane machinery, especially the endocytic and recycling pathways mediated by the ADP ribosylation factor guanine nucleotide exchange factor (ARF-GEF) GNOM. We assessed the role of the early secretory pathway in establishing PIN1 polarity in Arabidopsis thaliana by pharmacological and genetic approaches. We identified the compound endosidin 8 (ES8), which selectively interferes with PIN1 basal polarity without altering the polarity of apical proteins. ES8 alters the auxin distribution pattern in the root and induces a strong developmental phenotype, including reduced root length. The ARF-GEF-defective mutants gnom-like 1 (gnl1-1) and gnom (van7) are significantly resistant to ES8. The compound does not affect recycling or vacuolar trafficking of PIN1 but leads to its intracellular accumulation, resulting in loss of PIN1 basal polarity at the plasma membrane. Our data confirm a role for GNOM in endoplasmic reticulum (ER)-Golgi trafficking and reveal that a GNL1/GNOM-mediated early secretory pathway selectively regulates PIN1 basal polarity establishment in a manner essential for normal plant development.
@article{doyle_early_2015,
title = {An early secretory pathway mediated by {GNOM}-{LIKE} 1 and {GNOM} is essential for basal polarity establishment in {Arabidopsis} thaliana},
volume = {112},
issn = {1091-6490 (Electronic) 0027-8424 (Linking)},
url = {https://www.ncbi.nlm.nih.gov/pubmed/25646449},
doi = {10.1073/pnas.1424856112},
abstract = {Spatial regulation of the plant hormone indole-3-acetic acid (IAA, or auxin) is essential for plant development. Auxin gradient establishment is mediated by polarly localized auxin transporters, including PIN-FORMED (PIN) proteins. Their localization and abundance at the plasma membrane are tightly regulated by endomembrane machinery, especially the endocytic and recycling pathways mediated by the ADP ribosylation factor guanine nucleotide exchange factor (ARF-GEF) GNOM. We assessed the role of the early secretory pathway in establishing PIN1 polarity in Arabidopsis thaliana by pharmacological and genetic approaches. We identified the compound endosidin 8 (ES8), which selectively interferes with PIN1 basal polarity without altering the polarity of apical proteins. ES8 alters the auxin distribution pattern in the root and induces a strong developmental phenotype, including reduced root length. The ARF-GEF-defective mutants gnom-like 1 (gnl1-1) and gnom (van7) are significantly resistant to ES8. The compound does not affect recycling or vacuolar trafficking of PIN1 but leads to its intracellular accumulation, resulting in loss of PIN1 basal polarity at the plasma membrane. Our data confirm a role for GNOM in endoplasmic reticulum (ER)-Golgi trafficking and reveal that a GNL1/GNOM-mediated early secretory pathway selectively regulates PIN1 basal polarity establishment in a manner essential for normal plant development.},
language = {en},
number = {7},
urldate = {2021-06-07},
journal = {Proc Natl Acad Sci U S A},
author = {Doyle, S. M. and Haeger, A. and Vain, T. and Rigal, A. and Viotti, C. and Langowska, M. and Ma, Q. and Friml, J. and Raikhel, N. V. and Hicks, G. R. and Robert, S.},
month = feb,
year = {2015},
note = {Edition: 2015/02/04},
keywords = {Arabidopsis Proteins/metabolism/*physiology, Arabidopsis/growth \& development/*physiology, Endocytosis, Guanine Nucleotide Exchange Factors/*physiology, Membrane Transport Proteins/metabolism, PIN auxin transporters, Protein Transport, chemical genomics, endomembrane trafficking, plasma membrane protein polarity},
pages = {E806--15},
}
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Their localization and abundance at the plasma membrane are tightly regulated by endomembrane machinery, especially the endocytic and recycling pathways mediated by the ADP ribosylation factor guanine nucleotide exchange factor (ARF-GEF) GNOM. We assessed the role of the early secretory pathway in establishing PIN1 polarity in Arabidopsis thaliana by pharmacological and genetic approaches. We identified the compound endosidin 8 (ES8), which selectively interferes with PIN1 basal polarity without altering the polarity of apical proteins. ES8 alters the auxin distribution pattern in the root and induces a strong developmental phenotype, including reduced root length. The ARF-GEF-defective mutants gnom-like 1 (gnl1-1) and gnom (van7) are significantly resistant to ES8. The compound does not affect recycling or vacuolar trafficking of PIN1 but leads to its intracellular accumulation, resulting in loss of PIN1 basal polarity at the plasma membrane. 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