Biophysical models reveal the relative importance of transporter proteins and impermeant anions in chloride homeostasis. Düsterwald, K., M., Currin, C., B., Burman, R., J., Akerman, C., J., Kay, A., R., & Raimondo, J., V. eLife, 9, 2018.
Website doi abstract bibtex 2 downloads Fast synaptic inhibition in the nervous system depends on the transmembrane flux of Cl- ions based on the neuronal Cl- driving force. Established theories regarding the determinants of Cl- driving force have recently been questioned. Here, we present biophysical models of Cl- homeostasis using the pump-leak model. Using numerical and novel analytic solutions, we demonstrate that the Na+/K+-ATPase, ion conductances, impermeant anions, electrodiffusion, water fluxes and cation-chloride cotransporters (CCCs) play roles in setting the Cl- driving force. Our models, together with experimental validation, show that while impermeant anions can contribute to setting [Cl-]i in neurons, they have a negligible effect on the driving force for Cl- locally and cell-wide. In contrast, we demonstrate that CCCs are well-suited for modulating Cl- driving force and hence inhibitory signaling in neurons. Our findings reconcile recent experimental findings and provide a framework for understanding the interplay of different chloride regulatory processes in neurons.
@article{
title = {Biophysical models reveal the relative importance of transporter proteins and impermeant anions in chloride homeostasis},
type = {article},
year = {2018},
volume = {7},
websites = {https://elifesciences.org/articles/39575},
month = {9},
day = {27},
id = {ef06980f-e4cf-3cb9-a7e5-ce73c209b9e8},
created = {2018-10-27T20:40:55.303Z},
accessed = {2018-10-27},
file_attached = {true},
profile_id = {14badb0b-89a9-3047-a69a-683d5240354c},
last_modified = {2019-10-31T15:55:19.149Z},
read = {true},
starred = {false},
authored = {true},
confirmed = {true},
hidden = {false},
citation_key = {Dusterwald2018},
folder_uuids = {bf6cbabe-7a6a-4003-9dd0-f1c24e66ab8a},
private_publication = {false},
abstract = {Fast synaptic inhibition in the nervous system depends on the transmembrane flux of Cl- ions based on the neuronal Cl- driving force. Established theories regarding the determinants of Cl- driving force have recently been questioned. Here, we present biophysical models of Cl- homeostasis using the pump-leak model. Using numerical and novel analytic solutions, we demonstrate that the Na+/K+-ATPase, ion conductances, impermeant anions, electrodiffusion, water fluxes and cation-chloride cotransporters (CCCs) play roles in setting the Cl- driving force. Our models, together with experimental validation, show that while impermeant anions can contribute to setting [Cl-]i in neurons, they have a negligible effect on the driving force for Cl- locally and cell-wide. In contrast, we demonstrate that CCCs are well-suited for modulating Cl- driving force and hence inhibitory signaling in neurons. Our findings reconcile recent experimental findings and provide a framework for understanding the interplay of different chloride regulatory processes in neurons.},
bibtype = {article},
author = {Düsterwald, Kira M and Currin, Christopher B and Burman, Richard J and Akerman, Colin J and Kay, Alan R and Raimondo, Joseph V},
doi = {10.7554/eLife.39575},
journal = {eLife}
}
Downloads: 2
{"_id":"naXNHXge4zqprqMng","bibbaseid":"dsterwald-currin-burman-akerman-kay-raimondo-biophysicalmodelsrevealtherelativeimportanceoftransporterproteinsandimpermeantanionsinchloridehomeostasis-2018","downloads":2,"creationDate":"2019-02-13T11:06:59.741Z","title":"Biophysical models reveal the relative importance of transporter proteins and impermeant anions in chloride homeostasis","author_short":["Düsterwald, K., M.","Currin, C., B.","Burman, R., J.","Akerman, C., J.","Kay, A., R.","Raimondo, J., V."],"year":2018,"bibtype":"article","biburl":"https://bibbase.org/service/mendeley/14badb0b-89a9-3047-a69a-683d5240354c","bibdata":{"title":"Biophysical models reveal the relative importance of transporter proteins and impermeant anions in chloride homeostasis","type":"article","year":"2018","volume":"7","websites":"https://elifesciences.org/articles/39575","month":"9","day":"27","id":"ef06980f-e4cf-3cb9-a7e5-ce73c209b9e8","created":"2018-10-27T20:40:55.303Z","accessed":"2018-10-27","file_attached":"true","profile_id":"14badb0b-89a9-3047-a69a-683d5240354c","last_modified":"2019-10-31T15:55:19.149Z","read":"true","starred":false,"authored":"true","confirmed":"true","hidden":false,"citation_key":"Dusterwald2018","folder_uuids":"bf6cbabe-7a6a-4003-9dd0-f1c24e66ab8a","private_publication":false,"abstract":"Fast synaptic inhibition in the nervous system depends on the transmembrane flux of Cl- ions based on the neuronal Cl- driving force. Established theories regarding the determinants of Cl- driving force have recently been questioned. Here, we present biophysical models of Cl- homeostasis using the pump-leak model. Using numerical and novel analytic solutions, we demonstrate that the Na+/K+-ATPase, ion conductances, impermeant anions, electrodiffusion, water fluxes and cation-chloride cotransporters (CCCs) play roles in setting the Cl- driving force. Our models, together with experimental validation, show that while impermeant anions can contribute to setting [Cl-]i in neurons, they have a negligible effect on the driving force for Cl- locally and cell-wide. In contrast, we demonstrate that CCCs are well-suited for modulating Cl- driving force and hence inhibitory signaling in neurons. Our findings reconcile recent experimental findings and provide a framework for understanding the interplay of different chloride regulatory processes in neurons.","bibtype":"article","author":"Düsterwald, Kira M and Currin, Christopher B and Burman, Richard J and Akerman, Colin J and Kay, Alan R and Raimondo, Joseph V","doi":"10.7554/eLife.39575","journal":"eLife","bibtex":"@article{\n title = {Biophysical models reveal the relative importance of transporter proteins and impermeant anions in chloride homeostasis},\n type = {article},\n year = {2018},\n volume = {7},\n websites = {https://elifesciences.org/articles/39575},\n month = {9},\n day = {27},\n id = {ef06980f-e4cf-3cb9-a7e5-ce73c209b9e8},\n created = {2018-10-27T20:40:55.303Z},\n accessed = {2018-10-27},\n file_attached = {true},\n profile_id = {14badb0b-89a9-3047-a69a-683d5240354c},\n last_modified = {2019-10-31T15:55:19.149Z},\n read = {true},\n starred = {false},\n authored = {true},\n confirmed = {true},\n hidden = {false},\n citation_key = {Dusterwald2018},\n folder_uuids = {bf6cbabe-7a6a-4003-9dd0-f1c24e66ab8a},\n private_publication = {false},\n abstract = {Fast synaptic inhibition in the nervous system depends on the transmembrane flux of Cl- ions based on the neuronal Cl- driving force. Established theories regarding the determinants of Cl- driving force have recently been questioned. Here, we present biophysical models of Cl- homeostasis using the pump-leak model. Using numerical and novel analytic solutions, we demonstrate that the Na+/K+-ATPase, ion conductances, impermeant anions, electrodiffusion, water fluxes and cation-chloride cotransporters (CCCs) play roles in setting the Cl- driving force. Our models, together with experimental validation, show that while impermeant anions can contribute to setting [Cl-]i in neurons, they have a negligible effect on the driving force for Cl- locally and cell-wide. In contrast, we demonstrate that CCCs are well-suited for modulating Cl- driving force and hence inhibitory signaling in neurons. Our findings reconcile recent experimental findings and provide a framework for understanding the interplay of different chloride regulatory processes in neurons.},\n bibtype = {article},\n author = {Düsterwald, Kira M and Currin, Christopher B and Burman, Richard J and Akerman, Colin J and Kay, Alan R and Raimondo, Joseph V},\n doi = {10.7554/eLife.39575},\n journal = {eLife}\n}","author_short":["Düsterwald, K., M.","Currin, C., B.","Burman, R., J.","Akerman, C., J.","Kay, A., R.","Raimondo, J., V."],"urls":{"Website":"https://elifesciences.org/articles/39575"},"biburl":"https://bibbase.org/service/mendeley/14badb0b-89a9-3047-a69a-683d5240354c","bibbaseid":"dsterwald-currin-burman-akerman-kay-raimondo-biophysicalmodelsrevealtherelativeimportanceoftransporterproteinsandimpermeantanionsinchloridehomeostasis-2018","role":"author","metadata":{"authorlinks":{"currin, c":"https://bibbase.org/service/mendeley/14badb0b-89a9-3047-a69a-683d5240354c"}},"downloads":2},"search_terms":["biophysical","models","reveal","relative","importance","transporter","proteins","impermeant","anions","chloride","homeostasis","düsterwald","currin","burman","akerman","kay","raimondo"],"keywords":[],"authorIDs":["4GRzhHm3jkczjXnhD","5TRegbppBcHSHh95r","5d976f91fff04bdb010002dd","5de834268cf0fbde01000007","5dfa2d031c2129de010000f9","5dfa4617ed5baede010000e4","5dfa4d61ed5baede01000182","5e09c812954ff4de01000099","5e10dd0e45c12cde01000026","5e1327c656233bde01000161","5e18aec0bd0cf5de01000075","5e21eaf159a877de01000090","5e25832cd31494de01000018","5e28552fc4828cde0100015e","5e296581661211de010000b9","5e2ee11d9033bede010000e3","5e32f074c1389bde010000ca","5e32fadec1389bde010002b6","5e330734200286df01000036","5e33486ee0067bde010000ca","5e33a76a17f2c9de0100010a","5e3aca33f2a00cdf0100018b","5e3bccaf657d0bdf0100011d","5e413543b7d170df0100002d","5e44ef0b03c52dde0100000a","5e4843cc00973ede0100005d","5e4d8b92d2986bde01000142","5e56c4e696127bde010000e4","5e579668cef9b7de0100013e","5e57d0c75d5d52de010001a4","5e5cf3fdd12a0ade010000c1","5e5fbe0d6b32b0f201000037","6yQZHdKBzMSyz8kHH","6z39eNYBTRzCqguED","735RSq9stADHg6Yo9","7ECknguKPkzHZ6DQr","7NbPLvgqKB6Qa6Pve","7yTJ7cR4E6SnCEbQH","ANyA4zpAxNpFaYiss","CpifsWbnt9WaX2YuZ","HFFtNK7MEswwXEfX8","KoyNZnBBmKWvhkpDT","P6nz2L6eRTuBvtQTp","PsoaYzSzj2soLsfky","Q5igLQrML9z3jHS6v","Q9FqGc8AAJA5NQdgE","QGaYL7M3QSQ3P6v6n","QSS67B7MzeR6Rz7DG","QZmKNMArjBLQtNpbD","QgiSgCeLY8zhBssBn","S6kPEp64fbfA5F9i5","SxGonwuM235DRmewA","Z8QXFDY4uwFunJcCn","apNBnwSvjMMyv2wcL","bqqvSoDyrxswmk2JP","cZAbP2RFisLcq4myk","cqzN6JEcxGZ8DZZSw","eDQbdyYmTZdgTjKer","h2qqrhSNGrYu2W39j","jRwtWoxjiPHAW5bQt","mJogcw7pae58FkAkt","nCeTthTuMYeMkSje7","qrb5im2hcok6EWf9T","sJ6aNArixmKraYX9i","sZTywBkdmx98zk3rX","srdLNeAWWqganDDAN","u9NmA6DWpppPdMiJE"],"dataSources":["wSSwpDnZRaKr65ojE","ya2CyA73rpZseyrZ8","4JtfuoMmGzmH3sgtu","2252seNhipfTmjEBQ"]}