@article{RN83,
   author = {Duncan, J. L. and Ahmad, R. N. and Danesi, H. and Slade, D. J. and Davalos, R. V. and Verbridge, S. S.},
   title = {Electro-antibacterial therapy (EAT) to enhance intracellular bacteria clearance in pancreatic cancer cells},
   journal = {Bioelectrochemistry},
   volume = {157},
   pages = {108669},
   note = {1878-562x
Duncan, Josie L
Ahmad, Raffae N
Danesi, Hunter
Slade, Daniel J
Davalos, Rafael V
Verbridge, Scott S
Journal Article
Netherlands
2024/02/21
Bioelectrochemistry. 2024 Feb 15;157:108669. doi: 10.1016/j.bioelechem.2024.108669.},
   abstract = {Intratumoral bacteria have been implicated in driving tumor progression, yet effective treatments to modulate the tumor microbiome remain limited. In this study, we investigate the use of electroporation in combination with metronidazole to enhance the clearance of intracellular Fusobacterium nucleatum within pancreatic cancer cells. We explore various parameters, including electric field strength, pulse width, and pulse number to assess the permeability of pancreatic cancer cells infected with F. nucleatum, compared to non-infected cells of the same type. We subsequently quantify the clearance of intracellular bacteria when these pulsing schemes are applied to a suspension of infected pancreatic cancer cells in the presence of metronidazole. Our results reveal distinct differences in cell permeability between infected and non-infected cells, identifying a unique biophysical marker for host cells infected with F. nucleatum. We demonstrate that the combinatorial use of electroporation and metronidazole significantly enhances the delivery of metronidazole into host cells, leading to more effective clearance of intracellular F. nucleatum compared to independent treatments; we term this novel approach Electro-Antibacterial Therapy (EAT). EAT holds promise as an innovative strategy for addressing intratumoral bacteria in pancreatic cancer, other malignancies, and potentially treatment-resistant infections, offering new avenues for therapeutic intervention.},
   keywords = {Antibiotic
Bacteria
Electroporation
Fusobacterium
Pulsed Electric Fields
Tumor Microbiome},
   ISSN = {1567-5394},
   DOI = {10.1016/j.bioelechem.2024.108669},
   year = {2024},
   type = {Journal Article}
}

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S."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Duncan"],"firstnames":["J.","L."],"suffixes":[]},{"propositions":[],"lastnames":["Ahmad"],"firstnames":["R.","N."],"suffixes":[]},{"propositions":[],"lastnames":["Danesi"],"firstnames":["H."],"suffixes":[]},{"propositions":[],"lastnames":["Slade"],"firstnames":["D.","J."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Verbridge"],"firstnames":["S.","S."],"suffixes":[]}],"title":"Electro-antibacterial therapy (EAT) to enhance intracellular bacteria clearance in pancreatic cancer cells","journal":"Bioelectrochemistry","volume":"157","pages":"108669","note":"1878-562x Duncan, Josie L Ahmad, Raffae N Danesi, Hunter Slade, Daniel J Davalos, Rafael V Verbridge, Scott S Journal Article Netherlands 2024/02/21 Bioelectrochemistry. 2024 Feb 15;157:108669. doi: 10.1016/j.bioelechem.2024.108669.","abstract":"Intratumoral bacteria have been implicated in driving tumor progression, yet effective treatments to modulate the tumor microbiome remain limited. In this study, we investigate the use of electroporation in combination with metronidazole to enhance the clearance of intracellular Fusobacterium nucleatum within pancreatic cancer cells. We explore various parameters, including electric field strength, pulse width, and pulse number to assess the permeability of pancreatic cancer cells infected with F. nucleatum, compared to non-infected cells of the same type. We subsequently quantify the clearance of intracellular bacteria when these pulsing schemes are applied to a suspension of infected pancreatic cancer cells in the presence of metronidazole. Our results reveal distinct differences in cell permeability between infected and non-infected cells, identifying a unique biophysical marker for host cells infected with F. nucleatum. We demonstrate that the combinatorial use of electroporation and metronidazole significantly enhances the delivery of metronidazole into host cells, leading to more effective clearance of intracellular F. nucleatum compared to independent treatments; we term this novel approach Electro-Antibacterial Therapy (EAT). EAT holds promise as an innovative strategy for addressing intratumoral bacteria in pancreatic cancer, other malignancies, and potentially treatment-resistant infections, offering new avenues for therapeutic intervention.","keywords":"Antibiotic Bacteria Electroporation Fusobacterium Pulsed Electric Fields Tumor Microbiome","issn":"1567-5394","doi":"10.1016/j.bioelechem.2024.108669","year":"2024","bibtex":"@article{RN83,\n author = {Duncan, J. L. and Ahmad, R. N. and Danesi, H. and Slade, D. J. and Davalos, R. V. and Verbridge, S. S.},\n title = {Electro-antibacterial therapy (EAT) to enhance intracellular bacteria clearance in pancreatic cancer cells},\n journal = {Bioelectrochemistry},\n volume = {157},\n pages = {108669},\n note = {1878-562x\nDuncan, Josie L\nAhmad, Raffae N\nDanesi, Hunter\nSlade, Daniel J\nDavalos, Rafael V\nVerbridge, Scott S\nJournal Article\nNetherlands\n2024/02/21\nBioelectrochemistry. 2024 Feb 15;157:108669. doi: 10.1016/j.bioelechem.2024.108669.},\n abstract = {Intratumoral bacteria have been implicated in driving tumor progression, yet effective treatments to modulate the tumor microbiome remain limited. In this study, we investigate the use of electroporation in combination with metronidazole to enhance the clearance of intracellular Fusobacterium nucleatum within pancreatic cancer cells. We explore various parameters, including electric field strength, pulse width, and pulse number to assess the permeability of pancreatic cancer cells infected with F. nucleatum, compared to non-infected cells of the same type. We subsequently quantify the clearance of intracellular bacteria when these pulsing schemes are applied to a suspension of infected pancreatic cancer cells in the presence of metronidazole. Our results reveal distinct differences in cell permeability between infected and non-infected cells, identifying a unique biophysical marker for host cells infected with F. nucleatum. We demonstrate that the combinatorial use of electroporation and metronidazole significantly enhances the delivery of metronidazole into host cells, leading to more effective clearance of intracellular F. nucleatum compared to independent treatments; we term this novel approach Electro-Antibacterial Therapy (EAT). EAT holds promise as an innovative strategy for addressing intratumoral bacteria in pancreatic cancer, other malignancies, and potentially treatment-resistant infections, offering new avenues for therapeutic intervention.},\n keywords = {Antibiotic\nBacteria\nElectroporation\nFusobacterium\nPulsed Electric Fields\nTumor Microbiome},\n ISSN = {1567-5394},\n DOI = {10.1016/j.bioelechem.2024.108669},\n year = {2024},\n type = {Journal Article}\n}\n\n","author_short":["Duncan, J. L.","Ahmad, R. N.","Danesi, H.","Slade, D. J.","Davalos, R. V.","Verbridge, S. S."],"key":"RN83","id":"RN83","bibbaseid":"duncan-ahmad-danesi-slade-davalos-verbridge-electroantibacterialtherapyeattoenhanceintracellularbacteriaclearanceinpancreaticcancercells-2024","role":"author","urls":{},"keyword":["Antibiotic Bacteria Electroporation Fusobacterium Pulsed Electric Fields Tumor Microbiome"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["antibiotic bacteria electroporation fusobacterium pulsed electric fields tumor microbiome"],"search_terms":["electro","antibacterial","therapy","eat","enhance","intracellular","bacteria","clearance","pancreatic","cancer","cells","duncan","ahmad","danesi","slade","davalos","verbridge"],"title":"Electro-antibacterial therapy (EAT) to enhance intracellular bacteria clearance in pancreatic cancer cells","year":2024}