@article{RN89,
   author = {Duncan, J. L. and Bloomfield, M. and Swami, N. and Cimini, D. and Davalos, R. V.},
   title = {High-Frequency Dielectrophoresis Reveals That Distinct Bio-Electric Signatures of Colorectal Cancer Cells Depend on Ploidy and Nuclear Volume},
   journal = {Micromachines (Basel)},
   volume = {14},
   number = {9},
   note = {2072-666x
Duncan, Josie L
Orcid: 0000-0002-4743-1540
Bloomfield, Mathew
Orcid: 0000-0002-1782-081x
Swami, Nathan
Orcid: 0000-0002-0492-1160
Cimini, Daniela
Orcid: 0000-0002-4082-4894
Davalos, Rafael V
Orcid: 0000-0003-1503-9509
F31 CA271763/CA/NCI NIH HHS/United States
R01 GM140042/GM/NIGMS NIH HHS/United States
Journal Article
Switzerland
2023/09/28
Micromachines (Basel). 2023 Sep 1;14(9):1723. doi: 10.3390/mi14091723.},
   abstract = {Aneuploidy, or an incorrect chromosome number, is ubiquitous among cancers. Whole-genome duplication, resulting in tetraploidy, often occurs during the evolution of aneuploid tumors. Cancers that evolve through a tetraploid intermediate tend to be highly aneuploid and are associated with poor patient prognosis. The identification and enrichment of tetraploid cells from mixed populations is necessary to understand the role these cells play in cancer progression. Dielectrophoresis (DEP), a label-free electrokinetic technique, can distinguish cells based on their intracellular properties when stimulated above 10 MHz, but DEP has not been shown to distinguish tetraploid and/or aneuploid cancer cells from mixed tumor cell populations. Here, we used high-frequency DEP to distinguish cell subpopulations that differ in ploidy and nuclear size under flow conditions. We used impedance analysis to quantify the level of voltage decay at high frequencies and its impact on the DEP force acting on the cell. High-frequency DEP distinguished diploid cells from tetraploid clones due to their size and intracellular composition at frequencies above 40 MHz. Our findings demonstrate that high-frequency DEP can be a useful tool for identifying and distinguishing subpopulations with nuclear differences to determine their roles in disease progression.},
   keywords = {aneuploidy
electrokinetics
microfluidics},
   ISSN = {2072-666X (Print)
2072-666x},
   DOI = {10.3390/mi14091723},
   year = {2023},
   type = {Journal Article}
}

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V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Duncan"],"firstnames":["J.","L."],"suffixes":[]},{"propositions":[],"lastnames":["Bloomfield"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Swami"],"firstnames":["N."],"suffixes":[]},{"propositions":[],"lastnames":["Cimini"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"High-Frequency Dielectrophoresis Reveals That Distinct Bio-Electric Signatures of Colorectal Cancer Cells Depend on Ploidy and Nuclear Volume","journal":"Micromachines (Basel)","volume":"14","number":"9","note":"2072-666x Duncan, Josie L Orcid: 0000-0002-4743-1540 Bloomfield, Mathew Orcid: 0000-0002-1782-081x Swami, Nathan Orcid: 0000-0002-0492-1160 Cimini, Daniela Orcid: 0000-0002-4082-4894 Davalos, Rafael V Orcid: 0000-0003-1503-9509 F31 CA271763/CA/NCI NIH HHS/United States R01 GM140042/GM/NIGMS NIH HHS/United States Journal Article Switzerland 2023/09/28 Micromachines (Basel). 2023 Sep 1;14(9):1723. doi: 10.3390/mi14091723.","abstract":"Aneuploidy, or an incorrect chromosome number, is ubiquitous among cancers. Whole-genome duplication, resulting in tetraploidy, often occurs during the evolution of aneuploid tumors. Cancers that evolve through a tetraploid intermediate tend to be highly aneuploid and are associated with poor patient prognosis. The identification and enrichment of tetraploid cells from mixed populations is necessary to understand the role these cells play in cancer progression. Dielectrophoresis (DEP), a label-free electrokinetic technique, can distinguish cells based on their intracellular properties when stimulated above 10 MHz, but DEP has not been shown to distinguish tetraploid and/or aneuploid cancer cells from mixed tumor cell populations. Here, we used high-frequency DEP to distinguish cell subpopulations that differ in ploidy and nuclear size under flow conditions. We used impedance analysis to quantify the level of voltage decay at high frequencies and its impact on the DEP force acting on the cell. High-frequency DEP distinguished diploid cells from tetraploid clones due to their size and intracellular composition at frequencies above 40 MHz. Our findings demonstrate that high-frequency DEP can be a useful tool for identifying and distinguishing subpopulations with nuclear differences to determine their roles in disease progression.","keywords":"aneuploidy electrokinetics microfluidics","issn":"2072-666X (Print) 2072-666x","doi":"10.3390/mi14091723","year":"2023","bibtex":"@article{RN89,\n author = {Duncan, J. L. and Bloomfield, M. and Swami, N. and Cimini, D. and Davalos, R. V.},\n title = {High-Frequency Dielectrophoresis Reveals That Distinct Bio-Electric Signatures of Colorectal Cancer Cells Depend on Ploidy and Nuclear Volume},\n journal = {Micromachines (Basel)},\n volume = {14},\n number = {9},\n note = {2072-666x\nDuncan, Josie L\nOrcid: 0000-0002-4743-1540\nBloomfield, Mathew\nOrcid: 0000-0002-1782-081x\nSwami, Nathan\nOrcid: 0000-0002-0492-1160\nCimini, Daniela\nOrcid: 0000-0002-4082-4894\nDavalos, Rafael V\nOrcid: 0000-0003-1503-9509\nF31 CA271763/CA/NCI NIH HHS/United States\nR01 GM140042/GM/NIGMS NIH HHS/United States\nJournal Article\nSwitzerland\n2023/09/28\nMicromachines (Basel). 2023 Sep 1;14(9):1723. doi: 10.3390/mi14091723.},\n abstract = {Aneuploidy, or an incorrect chromosome number, is ubiquitous among cancers. Whole-genome duplication, resulting in tetraploidy, often occurs during the evolution of aneuploid tumors. Cancers that evolve through a tetraploid intermediate tend to be highly aneuploid and are associated with poor patient prognosis. The identification and enrichment of tetraploid cells from mixed populations is necessary to understand the role these cells play in cancer progression. Dielectrophoresis (DEP), a label-free electrokinetic technique, can distinguish cells based on their intracellular properties when stimulated above 10 MHz, but DEP has not been shown to distinguish tetraploid and/or aneuploid cancer cells from mixed tumor cell populations. Here, we used high-frequency DEP to distinguish cell subpopulations that differ in ploidy and nuclear size under flow conditions. We used impedance analysis to quantify the level of voltage decay at high frequencies and its impact on the DEP force acting on the cell. High-frequency DEP distinguished diploid cells from tetraploid clones due to their size and intracellular composition at frequencies above 40 MHz. Our findings demonstrate that high-frequency DEP can be a useful tool for identifying and distinguishing subpopulations with nuclear differences to determine their roles in disease progression.},\n keywords = {aneuploidy\nelectrokinetics\nmicrofluidics},\n ISSN = {2072-666X (Print)\n2072-666x},\n DOI = {10.3390/mi14091723},\n year = {2023},\n type = {Journal Article}\n}\n\n","author_short":["Duncan, J. L.","Bloomfield, M.","Swami, N.","Cimini, D.","Davalos, R. V."],"key":"RN89","id":"RN89","bibbaseid":"duncan-bloomfield-swami-cimini-davalos-highfrequencydielectrophoresisrevealsthatdistinctbioelectricsignaturesofcolorectalcancercellsdependonploidyandnuclearvolume-2023","role":"author","urls":{},"keyword":["aneuploidy electrokinetics microfluidics"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["aneuploidy electrokinetics microfluidics"],"search_terms":["high","frequency","dielectrophoresis","reveals","distinct","bio","electric","signatures","colorectal","cancer","cells","depend","ploidy","nuclear","volume","duncan","bloomfield","swami","cimini","davalos"],"title":"High-Frequency Dielectrophoresis Reveals That Distinct Bio-Electric Signatures of Colorectal Cancer Cells Depend on Ploidy and Nuclear Volume","year":2023}