Evaluation of Luminex xTAG Gastrointestinal Pathogen Panel Assay for Detection of Multiple Diarrheal Pathogens in Fecal Samples in Vietnam. Duong, V. T., Phat, V. V., Tuyen, H. T., Dung, T. T. N., Trung, P. D., Minh, P. V., Tu, L. T. P., Campbell, J. I., Le Phuc, H., Ha, T. T. T., Ngoc, N. M., Huong, N. T. T., Tam, P. T. T., Huong, D. T., Xang, N. V., Dong, N., Phuong, L. T., Hung, N. V., Phu, B. D., Phuc, T. M., Thwaites, G. E., Vi, L. L., Rabaa, M. A., Thompson, C. N., & Baker, S. Journal of clinical microbiology, 54(4):1094–1100, April, 2016.
doi  abstract   bibtex   
Diarrheal disease is a complex syndrome that remains a leading cause of global childhood morbidity and mortality. The diagnosis of enteric pathogens in a timely and precise manner is important for making treatment decisions and informing public health policy, but accurate diagnosis is a major challenge in industrializing countries. Multiplex molecular diagnostic techniques may represent a significant improvement over classical approaches. We evaluated the Luminex xTAG gastrointestinal pathogen panel (GPP) assay for the detection of common enteric bacterial and viral pathogens in Vietnam. Microbiological culture and real-time PCR were used as gold standards. The tests were performed on 479 stool samples collected from people admitted to the hospital for diarrheal disease throughout Vietnam. Sensitivity and specificity were calculated for the xTAG GPP for the seven principal diarrheal etiologies. The sensitivity and specificity for the xTAG GPP were \textgreater88% for Shigellaspp.,Campylobacterspp., rotavirus, norovirus genotype 1/2 (GI/GII), and adenovirus compared to those of microbiological culture and/or real-time PCR. However, the specificity was low ( approximately 60%) for Salmonella species. Additionally, a number of important pathogens that are not identified in routine hospital procedures in this setting, such as Cryptosporidiumspp. and Clostridium difficile, were detected with the GPP. The use of the Luminex xTAG GPP for the detection of enteric pathogens in settings, like Vietnam, would dramatically improve the diagnostic accuracy and capacity of hospital laboratories, allowing for timely and appropriate therapy decisions and a wider understanding of the epidemiology of pathogens associated with severe diarrheal disease in low-resource settings.
@article{duong_evaluation_2016,
	title = {Evaluation of {Luminex} {xTAG} {Gastrointestinal} {Pathogen} {Panel} {Assay} for {Detection} of {Multiple} {Diarrheal} {Pathogens} in {Fecal} {Samples} in {Vietnam}.},
	volume = {54},
	copyright = {Copyright (c) 2016 Duong et al.},
	issn = {1098-660X 0095-1137},
	doi = {10.1128/JCM.03321-15},
	abstract = {Diarrheal disease is a complex syndrome that remains a leading cause of global childhood morbidity and mortality. The diagnosis of enteric pathogens in a timely and precise manner is important for making treatment decisions and informing public health policy, but accurate diagnosis is a major challenge in industrializing countries. Multiplex molecular diagnostic techniques may represent a significant improvement over classical approaches. We evaluated the Luminex xTAG gastrointestinal pathogen panel (GPP) assay for the detection of common enteric bacterial and viral pathogens in Vietnam. Microbiological culture  and real-time PCR were used as gold standards. The tests were performed on 479 stool samples collected from people admitted to the hospital for diarrheal disease throughout Vietnam. Sensitivity and specificity were calculated for the xTAG GPP for the seven principal diarrheal etiologies. The sensitivity and specificity for the xTAG GPP were {\textgreater}88\% for Shigellaspp.,Campylobacterspp., rotavirus, norovirus genotype 1/2 (GI/GII), and adenovirus compared to those of microbiological culture and/or real-time PCR. However, the specificity was low (  approximately 60\%) for Salmonella species. Additionally, a number of important pathogens that are not identified in routine hospital procedures in this setting, such as Cryptosporidiumspp. and Clostridium difficile, were detected with the GPP. The use of the Luminex xTAG GPP for the detection of enteric pathogens in settings, like Vietnam, would dramatically improve the diagnostic accuracy and capacity of hospital laboratories, allowing for timely and appropriate therapy decisions and a wider understanding of the epidemiology of pathogens associated with severe diarrheal disease in low-resource settings.},
	language = {eng},
	number = {4},
	journal = {Journal of clinical microbiology},
	author = {Duong, Vu Thuy and Phat, Voong Vinh and Tuyen, Ha Thanh and Dung, Tran Thi Ngoc and Trung, Pham Duc and Minh, Pham Van and Tu, Le Thi Phuong and Campbell, James I. and Le Phuc, Hoang and Ha, Ton Thi Thanh and Ngoc, Nguyen Minh and Huong, Nguyen Thi Thanh and Tam, Pham Thi Thanh and Huong, Dang Thao and Xang, Nguyen Van and Dong, Nguyen and Phuong, Le Thi and Hung, Nguyen Van and Phu, Bui Duc and Phuc, Tran My and Thwaites, Guy E. and Vi, Lu Lan and Rabaa, Maia A. and Thompson, Corinne N. and Baker, Stephen},
	month = apr,
	year = {2016},
	pmid = {26865681},
	pmcid = {PMC4809950},
	keywords = {Adolescent, Adult, Aged, Aged, 80 and over, Bacteria/classification/*isolation \& purification, Child, Child, Preschool, Diarrhea/*diagnosis, Feces/*microbiology/*virology, Female, Humans, Immunoassay/*methods, Infant, Infant, Newborn, Male, Middle Aged, Sensitivity and Specificity, Vietnam, Viruses/classification/*isolation \& purification, Young Adult},
	pages = {1094--1100},
}
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