Deposition of Polymer Particles with Fibrinogen Corona at Abiotic Surfaces under Flow Conditions. Żeliszewska, P., Wasilewska, M., Cieśla, M., & Adamczyk, Z. Molecules, 26(20):6299, oct, 2021. ![Deposition of Polymer Particles with Fibrinogen Corona at Abiotic Surfaces under Flow Conditions [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The deposition kinetics of polymer particles with fibrinogen molecule coronas at bare and poly-L-lysine (PLL) modified mica was studied using the microfluid impinging-jet cell. Basic physicochemical characteristics of fibrinogen and the particles were acquired using dynamic light scattering and the electrophoretic mobility methods, whereas the zeta potential of the substrates was determined using streaming potential measurements. Subsequently, an efficient method for the preparation of the particles with coronas, characterized by a controlled fibrinogen coverage, was developed. This enabled us to carry out measurements, which confirmed that the deposition kinetics of the particles at mica vanished at pH above 5. In contrast, the particle deposition of PLL modified mica was at maximum for pH above 5. It was shown that the deposition kinetics could be adequately analyzed in terms of the mean-field approach, analogously to the ordinary colloid particle behavior. This contrasts the fibrinogen molecule behavior, which efficiently adsorbs at negatively charged substrates for the entire range pHs up to 9.7. These results have practical significance for conducting label-free immunoassays governed by the specific antigen/antibody interactions.
@article{Zeliszewska2021,
abstract = {The deposition kinetics of polymer particles with fibrinogen molecule coronas at bare and poly-L-lysine (PLL) modified mica was studied using the microfluid impinging-jet cell. Basic physicochemical characteristics of fibrinogen and the particles were acquired using dynamic light scattering and the electrophoretic mobility methods, whereas the zeta potential of the substrates was determined using streaming potential measurements. Subsequently, an efficient method for the preparation of the particles with coronas, characterized by a controlled fibrinogen coverage, was developed. This enabled us to carry out measurements, which confirmed that the deposition kinetics of the particles at mica vanished at pH above 5. In contrast, the particle deposition of PLL modified mica was at maximum for pH above 5. It was shown that the deposition kinetics could be adequately analyzed in terms of the mean-field approach, analogously to the ordinary colloid particle behavior. This contrasts the fibrinogen molecule behavior, which efficiently adsorbs at negatively charged substrates for the entire range pHs up to 9.7. These results have practical significance for conducting label-free immunoassays governed by the specific antigen/antibody interactions.},
author = {{\.{Z}}eliszewska, Paulina and Wasilewska, Monika and Cie{\'{s}}la, Micha{\l} and Adamczyk, Zbigniew},
doi = {10.3390/molecules26206299},
issn = {1420-3049},
journal = {Molecules},
keywords = {adsorption of fibrinogen,corona,coronas,deposition of particles with,fibrinogen corona at,microfluidic impinging-jet cell,particles,zeta potential of protein},
month = {oct},
number = {20},
pages = {6299},
title = {{Deposition of Polymer Particles with Fibrinogen Corona at Abiotic Surfaces under Flow Conditions}},
url = {https://www.mdpi.com/1420-3049/26/20/6299},
volume = {26},
year = {2021}
}
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This enabled us to carry out measurements, which confirmed that the deposition kinetics of the particles at mica vanished at pH above 5. In contrast, the particle deposition of PLL modified mica was at maximum for pH above 5. It was shown that the deposition kinetics could be adequately analyzed in terms of the mean-field approach, analogously to the ordinary colloid particle behavior. This contrasts the fibrinogen molecule behavior, which efficiently adsorbs at negatively charged substrates for the entire range pHs up to 9.7. 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