Comparative Study of the Cellular Uptake and Intracellular Behavior of a Library of Cyclic Peptide-Polymer Nanotubes with Different Self-Assembling Properties. Ellacott, S. H., Sanchez-Cano, C., Mansfield, E. D. H., Rho, J. Y., Song, J., Peltier, R., & Perrier, S. BIOMACROMOLECULES, 22(2):710–722, February, 2021. doi abstract bibtex Particle shape has been described as a key factor in improving cell internalization and biodistribution among the different properties investigated for drug-delivery systems. In particular, tubular structures have been identified as promising candidates for improving drug delivery. Here, we investigate the influence of different design elements of cyclic peptide-polymer nanotubes (CPNTs) on cellular uptake including the nature and length of the polymer and the cyclic peptide building block. By varying the composition of these cyclic peptide-polymer conjugates, a library of CPNTs of lengths varying from a few to over a 150 nm were synthesized and characterized using scattering techniques (small-angle neutron scattering and static light scattering). In vitro studies with fluorescently labeled CPNTs have shown that nanotubes comprised of a single polymer arm with a size between 8 and 16 nm were the most efficiently taken up by three different mammalian cell lines. A mechanistic study on multicellular tumor spheroids has confirmed the ability of these compounds to penetrate to their core. Variations in the proportion of paracellular and transcellular uptake with the self-assembling potential insights about the behavior of CPNTs in cellular systems.
@article{ellacott_comparative_2021,
title = {Comparative {Study} of the {Cellular} {Uptake} and {Intracellular} {Behavior} of a {Library} of {Cyclic} {Peptide}-{Polymer} {Nanotubes} with {Different} {Self}-{Assembling} {Properties}},
volume = {22},
issn = {1525-7797},
doi = {10.1021/acs.biomac.0c01512},
abstract = {Particle shape has been described as a key factor in improving cell internalization and biodistribution among the different properties investigated for drug-delivery systems. In particular, tubular structures have been identified as promising candidates for improving drug delivery. Here, we investigate the influence of different design elements of cyclic peptide-polymer nanotubes (CPNTs) on cellular uptake including the nature and length of the polymer and the cyclic peptide building block. By varying the composition of these cyclic peptide-polymer conjugates, a library of CPNTs of lengths varying from a few to over a 150 nm were synthesized and characterized using scattering techniques (small-angle neutron scattering and static light scattering). In vitro studies with fluorescently labeled CPNTs have shown that nanotubes comprised of a single polymer arm with a size between 8 and 16 nm were the most efficiently taken up by three different mammalian cell lines. A mechanistic study on multicellular tumor spheroids has confirmed the ability of these compounds to penetrate to their core. Variations in the proportion of paracellular and transcellular uptake with the self-assembling potential insights about the behavior of CPNTs in cellular systems.},
number = {2},
urldate = {2021-03-21},
journal = {BIOMACROMOLECULES},
author = {Ellacott, Sean H. and Sanchez-Cano, Carlos and Mansfield, Edward D. H. and Rho, Julia Y. and Song, Ji-Inn and Peltier, Raoul and Perrier, Sebastien},
month = feb,
year = {2021},
pages = {710--722},
}
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