Identifying New Components Participating in the Secondary Cell Wall Formation of Vessel Elements in Zinnia and Arabidopsis

Identifying New Components Participating in the Secondary Cell Wall Formation of Vessel Elements in Zinnia and Arabidopsis. Endo, S., Pesquet, E., Yamaguchi, M., Tashiro, G., Sato, M., Toyooka, K., Nishikubo, N., Udagawa-Motose, M., Kubo, M., Fukuda, H., & Demura, T. The Plant Cell, 21(4):1155–1165, May, 2009.

Paper doi abstract bibtex

Abstract Xylem vessel elements are hollow cellular units that assemble end-to-end to form a continuous vessel throughout the plant body; the xylem vessel is strengthened by the xylem elements' reinforced secondary cell walls (SCWs). This work aims to unravel the contribution of unknown actors in xylem vessel differentiation using the model in vitro cell culture system of Zinnia elegans differentiating cell cultures and the model in vivo system of Arabidopsis thaliana plants. Tracheary Element Differentiation-Related6 (TED6) and TED7 were selected based on an RNA interference (RNAi) screen in the Zinnia system. RNAi reduction of TED6 and 7 delayed tracheary element (TE) differentiation and co-overexpression of TED6 and 7 increased TE differentiation in cultured Zinnia cells. Arabidopsis TED6 and 7 were expressed preferentially in differentiating vessel elements in seedlings. Aberrant SCW formation of root vessel elements was induced by transient RNAi of At TED7 alone and enhanced by inhibition of both TED6 and 7. Protein–protein interactions were demonstrated between TED6 and a subunit of the SCW-related cellulose synthase complex. Our strategy has succeeded in finding two novel components in SCW formation and has opened the door for in-depth analysis of their molecular functions.

@article{endo_identifying_2009,
	title = {Identifying {New} {Components} {Participating} in the {Secondary} {Cell} {Wall} {Formation} of {Vessel} {Elements} in \textit{{Zinnia}} and \textit{{Arabidopsis}}},
	volume = {21},
	issn = {1532-298X, 1040-4651},
	url = {https://academic.oup.com/plcell/article/21/4/1155/6095257},
	doi = {10/cmhrbv},
	abstract = {Abstract
            Xylem vessel elements are hollow cellular units that assemble end-to-end to form a continuous vessel throughout the plant body; the xylem vessel is strengthened by the xylem elements' reinforced secondary cell walls (SCWs). This work aims to unravel the contribution of unknown actors in xylem vessel differentiation using the model in vitro cell culture system of Zinnia elegans differentiating cell cultures and the model in vivo system of Arabidopsis thaliana plants. Tracheary Element  Differentiation-Related6 (TED6) and TED7 were selected based on an RNA interference (RNAi) screen in the Zinnia system. RNAi reduction of TED6 and 7 delayed tracheary element (TE) differentiation and co-overexpression of TED6 and 7 increased TE differentiation in cultured Zinnia cells. Arabidopsis TED6 and 7 were expressed preferentially in differentiating vessel elements in seedlings. Aberrant SCW formation of root vessel elements was induced by transient RNAi of At TED7 alone and enhanced by inhibition of both TED6 and 7. Protein–protein interactions were demonstrated between TED6 and a subunit of the SCW-related cellulose synthase complex. Our strategy has succeeded in finding two novel components in SCW formation and has opened the door for in-depth analysis of their molecular functions.},
	language = {en},
	number = {4},
	urldate = {2021-06-08},
	journal = {The Plant Cell},
	author = {Endo, Satoshi and Pesquet, Edouard and Yamaguchi, Masatoshi and Tashiro, Gen and Sato, Mayuko and Toyooka, Kiminori and Nishikubo, Nobuyuki and Udagawa-Motose, Makiko and Kubo, Minoru and Fukuda, Hiroo and Demura, Taku},
	month = may,
	year = {2009},
	pages = {1155--1165},
}

Downloads: 0

{"_id":"SDNvG9GH2BZPuPyEx","bibbaseid":"endo-pesquet-yamaguchi-tashiro-sato-toyooka-nishikubo-udagawamotose-etal-identifyingnewcomponentsparticipatinginthesecondarycellwallformationofvesselelementsinizinniaiandiarabidopsisi-2009","author_short":["Endo, S.","Pesquet, E.","Yamaguchi, M.","Tashiro, G.","Sato, M.","Toyooka, K.","Nishikubo, N.","Udagawa-Motose, M.","Kubo, M.","Fukuda, H.","Demura, T."],"bibdata":{"bibtype":"article","type":"article","title":"Identifying New Components Participating in the Secondary Cell Wall Formation of Vessel Elements in Zinnia and Arabidopsis","volume":"21","issn":"1532-298X, 1040-4651","url":"https://academic.oup.com/plcell/article/21/4/1155/6095257","doi":"10/cmhrbv","abstract":"Abstract Xylem vessel elements are hollow cellular units that assemble end-to-end to form a continuous vessel throughout the plant body; the xylem vessel is strengthened by the xylem elements' reinforced secondary cell walls (SCWs). This work aims to unravel the contribution of unknown actors in xylem vessel differentiation using the model in vitro cell culture system of Zinnia elegans differentiating cell cultures and the model in vivo system of Arabidopsis thaliana plants. Tracheary Element Differentiation-Related6 (TED6) and TED7 were selected based on an RNA interference (RNAi) screen in the Zinnia system. RNAi reduction of TED6 and 7 delayed tracheary element (TE) differentiation and co-overexpression of TED6 and 7 increased TE differentiation in cultured Zinnia cells. Arabidopsis TED6 and 7 were expressed preferentially in differentiating vessel elements in seedlings. Aberrant SCW formation of root vessel elements was induced by transient RNAi of At TED7 alone and enhanced by inhibition of both TED6 and 7. Protein–protein interactions were demonstrated between TED6 and a subunit of the SCW-related cellulose synthase complex. Our strategy has succeeded in finding two novel components in SCW formation and has opened the door for in-depth analysis of their molecular functions.","language":"en","number":"4","urldate":"2021-06-08","journal":"The Plant Cell","author":[{"propositions":[],"lastnames":["Endo"],"firstnames":["Satoshi"],"suffixes":[]},{"propositions":[],"lastnames":["Pesquet"],"firstnames":["Edouard"],"suffixes":[]},{"propositions":[],"lastnames":["Yamaguchi"],"firstnames":["Masatoshi"],"suffixes":[]},{"propositions":[],"lastnames":["Tashiro"],"firstnames":["Gen"],"suffixes":[]},{"propositions":[],"lastnames":["Sato"],"firstnames":["Mayuko"],"suffixes":[]},{"propositions":[],"lastnames":["Toyooka"],"firstnames":["Kiminori"],"suffixes":[]},{"propositions":[],"lastnames":["Nishikubo"],"firstnames":["Nobuyuki"],"suffixes":[]},{"propositions":[],"lastnames":["Udagawa-Motose"],"firstnames":["Makiko"],"suffixes":[]},{"propositions":[],"lastnames":["Kubo"],"firstnames":["Minoru"],"suffixes":[]},{"propositions":[],"lastnames":["Fukuda"],"firstnames":["Hiroo"],"suffixes":[]},{"propositions":[],"lastnames":["Demura"],"firstnames":["Taku"],"suffixes":[]}],"month":"May","year":"2009","pages":"1155–1165","bibtex":"@article{endo_identifying_2009,\n\ttitle = {Identifying {New} {Components} {Participating} in the {Secondary} {Cell} {Wall} {Formation} of {Vessel} {Elements} in \\textit{{Zinnia}} and \\textit{{Arabidopsis}}},\n\tvolume = {21},\n\tissn = {1532-298X, 1040-4651},\n\turl = {https://academic.oup.com/plcell/article/21/4/1155/6095257},\n\tdoi = {10/cmhrbv},\n\tabstract = {Abstract\n Xylem vessel elements are hollow cellular units that assemble end-to-end to form a continuous vessel throughout the plant body; the xylem vessel is strengthened by the xylem elements' reinforced secondary cell walls (SCWs). This work aims to unravel the contribution of unknown actors in xylem vessel differentiation using the model in vitro cell culture system of Zinnia elegans differentiating cell cultures and the model in vivo system of Arabidopsis thaliana plants. Tracheary Element Differentiation-Related6 (TED6) and TED7 were selected based on an RNA interference (RNAi) screen in the Zinnia system. RNAi reduction of TED6 and 7 delayed tracheary element (TE) differentiation and co-overexpression of TED6 and 7 increased TE differentiation in cultured Zinnia cells. Arabidopsis TED6 and 7 were expressed preferentially in differentiating vessel elements in seedlings. Aberrant SCW formation of root vessel elements was induced by transient RNAi of At TED7 alone and enhanced by inhibition of both TED6 and 7. Protein–protein interactions were demonstrated between TED6 and a subunit of the SCW-related cellulose synthase complex. Our strategy has succeeded in finding two novel components in SCW formation and has opened the door for in-depth analysis of their molecular functions.},\n\tlanguage = {en},\n\tnumber = {4},\n\turldate = {2021-06-08},\n\tjournal = {The Plant Cell},\n\tauthor = {Endo, Satoshi and Pesquet, Edouard and Yamaguchi, Masatoshi and Tashiro, Gen and Sato, Mayuko and Toyooka, Kiminori and Nishikubo, Nobuyuki and Udagawa-Motose, Makiko and Kubo, Minoru and Fukuda, Hiroo and Demura, Taku},\n\tmonth = may,\n\tyear = {2009},\n\tpages = {1155--1165},\n}\n\n\n\n","author_short":["Endo, S.","Pesquet, E.","Yamaguchi, M.","Tashiro, G.","Sato, M.","Toyooka, K.","Nishikubo, N.","Udagawa-Motose, M.","Kubo, M.","Fukuda, H.","Demura, T."],"key":"endo_identifying_2009","id":"endo_identifying_2009","bibbaseid":"endo-pesquet-yamaguchi-tashiro-sato-toyooka-nishikubo-udagawamotose-etal-identifyingnewcomponentsparticipatinginthesecondarycellwallformationofvesselelementsinizinniaiandiarabidopsisi-2009","role":"author","urls":{"Paper":"https://academic.oup.com/plcell/article/21/4/1155/6095257"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["fvfkWcShg3Mybjoog","Tu3jPdZyJF3j547xT","9cGcv2t8pRzC92kzs","3zTPPmKj8BiTcpc6C"],"keywords":[],"search_terms":["identifying","new","components","participating","secondary","cell","wall","formation","vessel","elements","zinnia","arabidopsis","endo","pesquet","yamaguchi","tashiro","sato","toyooka","nishikubo","udagawa-motose","kubo","fukuda","demura"],"title":"Identifying New Components Participating in the Secondary Cell Wall Formation of Vessel Elements in Zinnia and Arabidopsis","year":2009}