High Detection Rates of Enteropathogens in Asymptomatic Children Attending Day Care. Enserink, R., Scholts, R., Bruijning-Verhagen, P., Duizer, E., Vennema, H., de Boer, R., Kortbeek, T., Roelfsema, J., Smit, H., Kooistra-Smid, M., & van Pelt, W. PLoS ONE, February, 2014. ![High Detection Rates of Enteropathogens in Asymptomatic Children Attending Day Care [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Background Gastroenteritis morbidity is high among children under the age of four, especially amongst those who attend day care. Objective To determine the prevalence of a range of enteropathogens in the intestinal flora of children attending day care and to relate their occurrence with characteristics of the sampled child and the sampling season. Methods We performed three years of enteropathogen surveillance in a network of 29 child day care centers in the Netherlands. The centers were instructed to take one fecal sample from ten randomly chosen children each month, regardless of gastrointestinal symptoms at time of sampling. All samples were analyzed for the molecular detection of 16 enteropathogenic bacteria, parasites and viruses by real-time multiplex PCR. Results Enteropathogens were detected in 78.0% of the 5197 fecal samples. Of the total, 95.4% of samples were obtained from children who had no gastroenteritis symptoms at time of sampling. Bacterial enteropathogens were detected most often (most prevalent EPEC, 19.9%), followed by parasitic enteropathogens (most prevalent: D. fragilis, 22.1%) and viral enteropathogens (most prevalent: norovirus, 9.5%). 4.6% of samples related to children that experienced symptoms of gastroenteritis at time of sampling. Only rotavirus and norovirus were significantly associated with gastroenteritis among day care attendees. Conclusions Our study indicates that asymptomatic infections with enteropathogens in day care attendees are not a rare event and that gastroenteritis caused by infections with these enteropathogens is only one expression of their presence.
@article{enserink_high_2014,
title = {High {Detection} {Rates} of {Enteropathogens} in {Asymptomatic} {Children} {Attending} {Day} {Care}},
volume = {9},
issn = {1932-6203},
url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933542/},
doi = {10.1371/journal.pone.0089496},
abstract = {Background
Gastroenteritis morbidity is high among children under the age of four, especially amongst those who attend day care.
Objective
To determine the prevalence of a range of enteropathogens in the intestinal flora of children attending day care and to relate their occurrence with characteristics of the sampled child and the sampling season.
Methods
We performed three years of enteropathogen surveillance in a network of 29 child day care centers in the Netherlands. The centers were instructed to take one fecal sample from ten randomly chosen children each month, regardless of gastrointestinal symptoms at time of sampling. All samples were analyzed for the molecular detection of 16 enteropathogenic bacteria, parasites and viruses by real-time multiplex PCR.
Results
Enteropathogens were detected in 78.0\% of the 5197 fecal samples. Of the total, 95.4\% of samples were obtained from children who had no gastroenteritis symptoms at time of sampling. Bacterial enteropathogens were detected most often (most prevalent EPEC, 19.9\%), followed by parasitic enteropathogens (most prevalent: D. fragilis, 22.1\%) and viral enteropathogens (most prevalent: norovirus, 9.5\%). 4.6\% of samples related to children that experienced symptoms of gastroenteritis at time of sampling. Only rotavirus and norovirus were significantly associated with gastroenteritis among day care attendees.
Conclusions
Our study indicates that asymptomatic infections with enteropathogens in day care attendees are not a rare event and that gastroenteritis caused by infections with these enteropathogens is only one expression of their presence.},
number = {2},
urldate = {2015-01-20},
journal = {PLoS ONE},
author = {Enserink, Remko and Scholts, Rianne and Bruijning-Verhagen, Patricia and Duizer, Erwin and Vennema, Harry and de Boer, Richard and Kortbeek, Titia and Roelfsema, Jeroen and Smit, Henriette and Kooistra-Smid, Mirjam and van Pelt, Wilfrid},
month = feb,
year = {2014},
pmid = {24586825},
pmcid = {PMC3933542},
}
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{"_id":"M9X7KgYMAPXShWXyP","bibbaseid":"enserink-scholts-bruijningverhagen-duizer-vennema-deboer-kortbeek-roelfsema-etal-highdetectionratesofenteropathogensinasymptomaticchildrenattendingdaycare-2014","author_short":["Enserink, R.","Scholts, R.","Bruijning-Verhagen, P.","Duizer, E.","Vennema, H.","de Boer, R.","Kortbeek, T.","Roelfsema, J.","Smit, H.","Kooistra-Smid, M.","van Pelt, W."],"bibdata":{"bibtype":"article","type":"article","title":"High Detection Rates of Enteropathogens in Asymptomatic Children Attending Day Care","volume":"9","issn":"1932-6203","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933542/","doi":"10.1371/journal.pone.0089496","abstract":"Background Gastroenteritis morbidity is high among children under the age of four, especially amongst those who attend day care. Objective To determine the prevalence of a range of enteropathogens in the intestinal flora of children attending day care and to relate their occurrence with characteristics of the sampled child and the sampling season. Methods We performed three years of enteropathogen surveillance in a network of 29 child day care centers in the Netherlands. The centers were instructed to take one fecal sample from ten randomly chosen children each month, regardless of gastrointestinal symptoms at time of sampling. All samples were analyzed for the molecular detection of 16 enteropathogenic bacteria, parasites and viruses by real-time multiplex PCR. Results Enteropathogens were detected in 78.0% of the 5197 fecal samples. Of the total, 95.4% of samples were obtained from children who had no gastroenteritis symptoms at time of sampling. Bacterial enteropathogens were detected most often (most prevalent EPEC, 19.9%), followed by parasitic enteropathogens (most prevalent: D. fragilis, 22.1%) and viral enteropathogens (most prevalent: norovirus, 9.5%). 4.6% of samples related to children that experienced symptoms of gastroenteritis at time of sampling. Only rotavirus and norovirus were significantly associated with gastroenteritis among day care attendees. Conclusions Our study indicates that asymptomatic infections with enteropathogens in day care attendees are not a rare event and that gastroenteritis caused by infections with these enteropathogens is only one expression of their presence.","number":"2","urldate":"2015-01-20","journal":"PLoS ONE","author":[{"propositions":[],"lastnames":["Enserink"],"firstnames":["Remko"],"suffixes":[]},{"propositions":[],"lastnames":["Scholts"],"firstnames":["Rianne"],"suffixes":[]},{"propositions":[],"lastnames":["Bruijning-Verhagen"],"firstnames":["Patricia"],"suffixes":[]},{"propositions":[],"lastnames":["Duizer"],"firstnames":["Erwin"],"suffixes":[]},{"propositions":[],"lastnames":["Vennema"],"firstnames":["Harry"],"suffixes":[]},{"propositions":["de"],"lastnames":["Boer"],"firstnames":["Richard"],"suffixes":[]},{"propositions":[],"lastnames":["Kortbeek"],"firstnames":["Titia"],"suffixes":[]},{"propositions":[],"lastnames":["Roelfsema"],"firstnames":["Jeroen"],"suffixes":[]},{"propositions":[],"lastnames":["Smit"],"firstnames":["Henriette"],"suffixes":[]},{"propositions":[],"lastnames":["Kooistra-Smid"],"firstnames":["Mirjam"],"suffixes":[]},{"propositions":["van"],"lastnames":["Pelt"],"firstnames":["Wilfrid"],"suffixes":[]}],"month":"February","year":"2014","pmid":"24586825","pmcid":"PMC3933542","bibtex":"@article{enserink_high_2014,\n\ttitle = {High {Detection} {Rates} of {Enteropathogens} in {Asymptomatic} {Children} {Attending} {Day} {Care}},\n\tvolume = {9},\n\tissn = {1932-6203},\n\turl = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933542/},\n\tdoi = {10.1371/journal.pone.0089496},\n\tabstract = {Background\nGastroenteritis morbidity is high among children under the age of four, especially amongst those who attend day care.\n\nObjective\nTo determine the prevalence of a range of enteropathogens in the intestinal flora of children attending day care and to relate their occurrence with characteristics of the sampled child and the sampling season.\n\nMethods\nWe performed three years of enteropathogen surveillance in a network of 29 child day care centers in the Netherlands. The centers were instructed to take one fecal sample from ten randomly chosen children each month, regardless of gastrointestinal symptoms at time of sampling. All samples were analyzed for the molecular detection of 16 enteropathogenic bacteria, parasites and viruses by real-time multiplex PCR.\n\nResults\nEnteropathogens were detected in 78.0\\% of the 5197 fecal samples. Of the total, 95.4\\% of samples were obtained from children who had no gastroenteritis symptoms at time of sampling. Bacterial enteropathogens were detected most often (most prevalent EPEC, 19.9\\%), followed by parasitic enteropathogens (most prevalent: D. fragilis, 22.1\\%) and viral enteropathogens (most prevalent: norovirus, 9.5\\%). 4.6\\% of samples related to children that experienced symptoms of gastroenteritis at time of sampling. Only rotavirus and norovirus were significantly associated with gastroenteritis among day care attendees.\n\nConclusions\nOur study indicates that asymptomatic infections with enteropathogens in day care attendees are not a rare event and that gastroenteritis caused by infections with these enteropathogens is only one expression of their presence.},\n\tnumber = {2},\n\turldate = {2015-01-20},\n\tjournal = {PLoS ONE},\n\tauthor = {Enserink, Remko and Scholts, Rianne and Bruijning-Verhagen, Patricia and Duizer, Erwin and Vennema, Harry and de Boer, Richard and Kortbeek, Titia and Roelfsema, Jeroen and Smit, Henriette and Kooistra-Smid, Mirjam and van Pelt, Wilfrid},\n\tmonth = feb,\n\tyear = {2014},\n\tpmid = {24586825},\n\tpmcid = {PMC3933542},\n}\n\n","author_short":["Enserink, R.","Scholts, R.","Bruijning-Verhagen, P.","Duizer, E.","Vennema, H.","de Boer, R.","Kortbeek, T.","Roelfsema, J.","Smit, H.","Kooistra-Smid, M.","van Pelt, W."],"key":"enserink_high_2014","id":"enserink_high_2014","bibbaseid":"enserink-scholts-bruijningverhagen-duizer-vennema-deboer-kortbeek-roelfsema-etal-highdetectionratesofenteropathogensinasymptomaticchildrenattendingdaycare-2014","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3933542/"},"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/robin.marlow","dataSources":["ix72eqAAMGCuupBaz"],"keywords":[],"search_terms":["high","detection","rates","enteropathogens","asymptomatic","children","attending","day","care","enserink","scholts","bruijning-verhagen","duizer","vennema","de boer","kortbeek","roelfsema","smit","kooistra-smid","van pelt"],"title":"High Detection Rates of Enteropathogens in Asymptomatic Children Attending Day Care","year":2014}